Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MW307_RS38290 Genome accession   NZ_CP095498
Coordinates   3380958..3382463 (+) Length   501 a.a.
NCBI ID   WP_059710755.1    Uniprot ID   -
Organism   Burkholderia cepacia strain BRDJ     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3375958..3387463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW307_RS38265 kdsD 3377190..3378173 (+) 984 WP_027789106.1 arabinose 5-phosphate isomerase KdsD -
  MW307_RS38270 - 3378173..3378709 (+) 537 WP_021162596.1 HAD family hydrolase -
  MW307_RS38275 lptC 3378713..3379327 (+) 615 WP_027789105.1 LPS export ABC transporter periplasmic protein LptC -
  MW307_RS38280 lptA 3379348..3380013 (+) 666 WP_021162598.1 lipopolysaccharide transport periplasmic protein LptA -
  MW307_RS38285 lptB 3380010..3380786 (+) 777 WP_021162599.1 LPS export ABC transporter ATP-binding protein -
  MW307_RS38290 rpoN/rpoN1 3380958..3382463 (+) 1506 WP_059710755.1 RNA polymerase factor sigma-54 Machinery gene
  MW307_RS38295 raiA 3382622..3382981 (+) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  MW307_RS38300 - 3383150..3383689 (+) 540 WP_034195946.1 PTS sugar transporter subunit IIA -
  MW307_RS38305 hprK 3383821..3384789 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  MW307_RS38310 rapZ 3384865..3385773 (+) 909 WP_021162602.1 RNase adapter RapZ -
  MW307_RS38315 - 3385801..3386436 (+) 636 WP_027789102.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54857.99 Da        Isoelectric Point: 4.8767

>NTDB_id=677220 MW307_RS38290 WP_059710755.1 3380958..3382463(+) (rpoN/rpoN1) [Burkholderia cepacia strain BRDJ]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPTA
TNSSSSSSERAERDEPAGADEYDGYASGDGNDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHTLIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=677220 MW307_RS38290 WP_059710755.1 3380958..3382463(+) (rpoN/rpoN1) [Burkholderia cepacia strain BRDJ]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TAGCGAGCCCGCTGCGTGTCGCAGCGGACGGATCGTTGATCGCGCAGACGCCTCCCGCCCAGAACACCGAGCCCACTGCG
ACAAACAGCAGCAGTTCGTCGAGCGAGCGCGCCGAGCGCGACGAACCGGCCGGCGCGGACGAATACGACGGTTACGCGTC
CGGCGACGGCAACGACGGCCCGCAGTGGAATCTCGACGAATTCGGCCGCGCGAGCGGCGCATCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAAATCGACTGCGACGAACTGAATGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGCGCCCGCTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACACGCACGCTC
GCACTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACACGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAAGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGCTCGCTCAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCGATTGTCG
AGCGTCAAAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCGTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGGTTCGTGGTCGCGCGC
CGCACGGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.182

100

0.689