Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MU539_RS10665 Genome accession   NZ_CP095384
Coordinates   2281066..2282430 (-) Length   454 a.a.
NCBI ID   WP_005686111.1    Uniprot ID   A0A2A5L5Z5
Organism   Lacticaseibacillus rhamnosus strain VHProbi M14     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2276066..2287430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MU539_RS10640 (MU539_10615) - 2276322..2276708 (-) 387 WP_033571722.1 GntR family transcriptional regulator -
  MU539_RS10645 (MU539_10620) - 2276900..2278216 (-) 1317 WP_257585642.1 MucBP domain-containing protein -
  MU539_RS10650 (MU539_10625) - 2278500..2278916 (-) 417 WP_080738571.1 ABC transporter permease -
  MU539_RS10655 (MU539_10630) - 2278942..2279841 (+) 900 WP_033571724.1 helix-turn-helix domain-containing protein -
  MU539_RS10660 (MU539_10635) - 2279930..2281045 (-) 1116 WP_005686113.1 PIN/TRAM domain-containing protein -
  MU539_RS10665 (MU539_10640) radA 2281066..2282430 (-) 1365 WP_005686111.1 DNA repair protein RadA Machinery gene
  MU539_RS10670 (MU539_10645) - 2282450..2282989 (-) 540 WP_005686110.1 dUTP diphosphatase -
  MU539_RS10675 (MU539_10650) - 2283507..2283797 (+) 291 WP_005686109.1 GNAT family N-acetyltransferase -
  MU539_RS10680 (MU539_10655) - 2283967..2285286 (+) 1320 WP_014571562.1 aminopeptidase C -
  MU539_RS10685 (MU539_10660) - 2285431..2286777 (+) 1347 WP_005714538.1 C1 family peptidase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49157.58 Da        Isoelectric Point: 8.3128

>NTDB_id=676636 MU539_RS10665 WP_005686111.1 2281066..2282430(-) (radA) [Lacticaseibacillus rhamnosus strain VHProbi M14]
MAKAKTQYVCQNCGYISATYLGRCPNCGGWNTLVEETVSSTKSAPRQTRTGSKVKPTRMDEVTVTKETRVKTGLAELNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSGQLASTGGTVLYVSGEESASQIKMRAGRLGVANSGMYLYPETDMPSIENVI
DQMQPDYVVIDSVQTMNVPDMNSAVGSVAQIREVTAELMRIAKSKGVTIFIVGHVTKEGAIAGPKILEHMVDTVLYFEGD
MHHTYRILRSVKNRFGSTNEIGIFEMHQNGLQEVANPSEIFLEERLAGATGSAVVVSMEGTRPILVEIQALISPTMYGNA
KRTSSGLDHNRVSLIMAVLEKRANLMLQNQDAYLKATGGVKLDEPAIDLAMAVAIASSYRDKEIPPTDCFVGEIGLTGEV
RRVNRIEDRVKEAAKLGFKRIFVPRNNLQGWRAPKDIQVIGVTSIAEALHKVFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=676636 MU539_RS10665 WP_005686111.1 2281066..2282430(-) (radA) [Lacticaseibacillus rhamnosus strain VHProbi M14]
ATGGCGAAAGCAAAAACACAATATGTATGTCAAAATTGCGGTTATATTTCGGCGACTTATTTAGGCCGTTGTCCAAACTG
CGGCGGCTGGAATACGCTCGTCGAAGAAACCGTTAGTTCAACTAAATCGGCGCCGCGGCAAACGCGCACCGGGAGCAAGG
TTAAGCCCACACGCATGGATGAGGTCACGGTGACCAAAGAAACCCGGGTTAAAACAGGCTTGGCCGAGTTGAATCGCGTG
CTTGGCGGCGGTGTGGTTCCGGGCTCGTTAGTTCTGATTGGCGGTGATCCCGGCATTGGCAAGTCAACCTTGCTGCTGCA
GGTATCTGGCCAACTAGCTAGCACAGGTGGCACGGTGTTGTACGTTTCAGGTGAAGAAAGTGCCAGCCAGATTAAAATGC
GGGCAGGGCGGCTAGGGGTTGCCAACTCCGGCATGTATTTGTATCCCGAAACCGACATGCCAAGCATCGAAAACGTGATT
GACCAGATGCAACCGGATTATGTGGTGATTGACTCGGTTCAAACCATGAATGTGCCGGACATGAATTCGGCAGTCGGATC
GGTTGCCCAAATTCGGGAAGTTACCGCCGAATTGATGCGCATTGCCAAGTCAAAAGGTGTGACGATTTTTATCGTTGGCC
ACGTGACGAAGGAAGGCGCCATTGCCGGGCCCAAGATTCTTGAGCATATGGTGGACACCGTGCTTTATTTTGAAGGCGAT
ATGCATCATACCTATCGCATTTTAAGGTCGGTCAAGAATCGGTTCGGCTCTACTAACGAAATCGGCATTTTTGAAATGCA
TCAAAATGGCTTACAGGAAGTCGCCAACCCCTCGGAAATTTTCTTAGAAGAGCGGCTGGCCGGTGCAACTGGCTCGGCGG
TGGTGGTTTCGATGGAAGGCACCCGTCCGATTCTAGTTGAAATTCAGGCACTTATCAGCCCGACCATGTATGGCAATGCC
AAACGTACGAGCAGCGGCTTGGATCACAACCGCGTCAGCCTGATTATGGCCGTGCTGGAAAAACGTGCCAATTTGATGTT
GCAAAATCAGGACGCTTACTTGAAAGCAACTGGTGGCGTTAAGCTGGATGAGCCGGCGATTGACTTGGCAATGGCAGTGG
CAATTGCCTCGTCATACCGCGACAAGGAAATCCCGCCGACTGATTGTTTTGTCGGGGAAATTGGCTTAACCGGTGAAGTG
CGGCGGGTGAATCGCATTGAAGACCGGGTGAAAGAGGCGGCTAAGTTAGGGTTTAAACGAATTTTTGTGCCGCGAAATAA
CCTGCAAGGCTGGCGTGCGCCTAAAGATATTCAGGTTATTGGCGTCACAAGCATTGCCGAAGCGCTGCATAAGGTTTTTA
ATTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-427)

AAA domain (AAA_25)

(67-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A5L5Z5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

68.433

99.78

0.683
  radA Streptococcus pneumoniae Rx1

68.433

99.78

0.683
  radA Streptococcus pneumoniae D39

68.433

99.78

0.683
  radA Streptococcus pneumoniae R6

68.433

99.78

0.683
  radA Streptococcus pneumoniae TIGR4

68.433

99.78

0.683
  radA Streptococcus mitis SK321

68.212

99.78

0.681
  radA Bacillus subtilis subsp. subtilis str. 168

64.912

100

0.652