Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   MWG54_RS25485 Genome accession   NZ_CP095377
Coordinates   4938242..4939591 (-) Length   449 a.a.
NCBI ID   WP_246961607.1    Uniprot ID   -
Organism   Bacillus cereus strain SEM-15     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4933242..4944591
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWG54_RS25450 (MWG54_25450) - 4933294..4933989 (-) 696 WP_000466510.1 hypothetical protein -
  MWG54_RS25455 (MWG54_25455) - 4933976..4934764 (-) 789 WP_000120044.1 hypothetical protein -
  MWG54_RS25460 (MWG54_25460) - 4934792..4935353 (-) 562 Protein_4957 hypothetical protein -
  MWG54_RS25465 (MWG54_25465) - 4935471..4935944 (-) 474 WP_098581101.1 hypothetical protein -
  MWG54_RS25470 (MWG54_25470) raiA 4936349..4936891 (-) 543 WP_000671186.1 ribosome-associated translation inhibitor RaiA -
  MWG54_RS25475 (MWG54_25475) cspC 4937214..4937411 (-) 198 WP_001990088.1 cold shock protein CspC -
  MWG54_RS25480 (MWG54_25480) - 4937538..4938242 (-) 705 WP_246961605.1 ComF family protein -
  MWG54_RS25485 (MWG54_25485) comFA 4938242..4939591 (-) 1350 WP_246961607.1 ATP-dependent helicase ComFA Machinery gene
  MWG54_RS25490 (MWG54_25490) - 4939719..4941149 (-) 1431 WP_141538448.1 C40 family peptidase -
  MWG54_RS25495 (MWG54_25495) - 4941298..4941612 (-) 315 WP_000400857.1 helix-turn-helix domain-containing protein -
  MWG54_RS25500 (MWG54_25500) - 4941785..4942627 (-) 843 WP_000684725.1 DegV family protein -
  MWG54_RS25505 (MWG54_25505) - 4942864..4943499 (+) 636 WP_000926665.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 51066.01 Da        Isoelectric Point: 9.9772

>NTDB_id=676605 MWG54_RS25485 WP_246961607.1 4938242..4939591(-) (comFA) [Bacillus cereus strain SEM-15]
MLAGKQLLLEELSSDLQRELNDLKKKGEIVCVQGVKKKNSKYMCQRCGNVDRRLFASFLCKRCSKVCAYCRKCITMGRVS
ECAVLVRGIAERKREKNLNLLQWNGTLSTGQNLAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIQIAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADRMLQYAVKQAM
KEKAARIYLTATPDETWKRKLKQGKQKGVIVSGRYHRHPLPVPLFCWCGNWKKTLIHKRIPRVLLQWLQTYLNKKFPIFL
FVPHVRYIEEISLLLKPLNNRIEGVHAEDPGRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=676605 MWG54_RS25485 WP_246961607.1 4938242..4939591(-) (comFA) [Bacillus cereus strain SEM-15]
ATGCTAGCGGGGAAACAGTTGCTATTAGAAGAACTTTCTTCAGATTTACAGAGGGAATTAAACGATTTGAAAAAGAAGGG
AGAGATCGTATGTGTACAAGGTGTAAAAAAAAAGAATTCTAAATATATGTGCCAACGCTGCGGAAATGTAGATCGGCGGC
TATTTGCGTCGTTTTTATGTAAAAGGTGCAGTAAAGTGTGCGCATATTGCCGGAAGTGTATAACGATGGGGAGGGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAATTTAAACTTGTTACAGTGGAACGGGACGTT
GTCTACTGGCCAGAATTTGGCGGCGCAAGGAGTTATAGAGGCTATTAAGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGAATTAACGAAGCGCTTCAAAAGGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCACCGAGATTGCAAGAAGTATTTCCATATATACAGATAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTCGTTGCGACTACGCATCAATTATTACGTTATTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCTTTTCCATATTGTGCAGATCGAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAAAGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAACTTAAACAAGGTAAACAAAA
AGGTGTTATTGTTTCTGGACGATATCATCGTCATCCTTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
CCCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACATACTTAAATAAAAAATTCCCTATTTTTTTA
TTCGTCCCCCATGTGCGATATATAGAAGAAATAAGCTTGTTGTTAAAACCATTAAACAATCGAATTGAAGGTGTACATGC
AGAAGATCCAGGGAGAAAAGAAAAAGTAGCGGCTTTCAGAAAGGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTTGCAGTTTTAGGGGCGGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAGGTCATTTATTTTCACTATGGCAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(313-408)

Type III restriction enzyme, res subunit (ResIII)

(107-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

51.131

98.441

0.503
  comFA Latilactobacillus sakei subsp. sakei 23K

41.687

89.755

0.374