Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MWG54_RS20900 Genome accession   NZ_CP095377
Coordinates   4086169..4087212 (-) Length   347 a.a.
NCBI ID   WP_246961224.1    Uniprot ID   -
Organism   Bacillus cereus strain SEM-15     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4081169..4092212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWG54_RS20855 (MWG54_20855) - 4081599..4081799 (-) 201 WP_246961220.1 YqzE family protein -
  MWG54_RS20860 (MWG54_20860) - 4081838..4082335 (-) 498 WP_000836650.1 shikimate kinase -
  MWG54_RS20865 (MWG54_20865) - 4082452..4083102 (-) 651 WP_000183860.1 2OG-Fe(II) oxygenase -
  MWG54_RS20870 (MWG54_20870) comGG 4083279..4083650 (-) 372 WP_246961222.1 competence type IV pilus minor pilin ComGG -
  MWG54_RS20875 (MWG54_20875) comGF 4083647..4084117 (-) 471 WP_000923099.1 competence type IV pilus minor pilin ComGF -
  MWG54_RS20880 (MWG54_20880) - 4084087..4084389 (-) 303 WP_000829473.1 hypothetical protein -
  MWG54_RS20885 (MWG54_20885) comGD 4084382..4084837 (-) 456 WP_000813142.1 competence type IV pilus minor pilin ComGD -
  MWG54_RS20890 (MWG54_20890) comGC 4084834..4085133 (-) 300 WP_001178686.1 competence type IV pilus major pilin ComGC -
  MWG54_RS20895 (MWG54_20895) comGB 4085145..4086182 (-) 1038 WP_002101326.1 competence type IV pilus assembly protein ComGB -
  MWG54_RS20900 (MWG54_20900) comGA 4086169..4087212 (-) 1044 WP_246961224.1 competence type IV pilus ATPase ComGA Machinery gene
  MWG54_RS20905 (MWG54_20905) - 4087418..4088113 (+) 696 WP_000434124.1 helix-turn-helix domain-containing protein -
  MWG54_RS20910 (MWG54_20910) - 4088239..4088481 (+) 243 WP_000440721.1 DUF2626 domain-containing protein -
  MWG54_RS20915 (MWG54_20915) - 4088588..4089982 (+) 1395 WP_001094330.1 L-cystine transporter -
  MWG54_RS20920 (MWG54_20920) - 4090062..4090454 (-) 393 WP_000875390.1 hypothetical protein -
  MWG54_RS20925 (MWG54_20925) - 4090542..4090757 (-) 216 WP_001008326.1 DUF3912 family protein -
  MWG54_RS20930 (MWG54_20930) - 4091010..4091321 (+) 312 WP_001093237.1 hypothetical protein -
  MWG54_RS20935 (MWG54_20935) - 4091356..4091844 (-) 489 WP_246961226.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39062.33 Da        Isoelectric Point: 8.3597

>NTDB_id=676594 MWG54_RS20900 WP_246961224.1 4086169..4087212(-) (comGA) [Bacillus cereus strain SEM-15]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTSQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSSFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGHELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=676594 MWG54_RS20900 WP_246961224.1 4086169..4087212(-) (comGA) [Bacillus cereus strain SEM-15]
ATGAATGGGATTGAAATTTTCGCGAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGCCAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGCTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTCATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGATCTGGGAAAACGACGACAATGTATGCATTATTAGAAGTA
ATTAGAAAGAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGGGATGAGGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTACTATATGGACATGAGTTAAAACAAGCACTTAAAGAAGCAGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.62

100

0.556