Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MWG54_RS19720 Genome accession   NZ_CP095377
Coordinates   3883637..3884071 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus cereus strain SEM-15     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3878637..3889071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWG54_RS19705 (MWG54_19705) - 3879815..3880372 (+) 558 WP_000062063.1 PadR family transcriptional regulator -
  MWG54_RS19710 (MWG54_19710) metE 3880884..3883172 (+) 2289 WP_246961068.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  MWG54_RS19715 (MWG54_19715) comJ 3883211..3883600 (-) 390 WP_000424030.1 competence protein ComJ -
  MWG54_RS19720 (MWG54_19720) nucA/comI 3883637..3884071 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  MWG54_RS19725 (MWG54_19725) - 3884298..3885047 (+) 750 WP_000388471.1 DUF3967 domain-containing protein -
  MWG54_RS19730 (MWG54_19730) kinB 3885086..3886360 (-) 1275 WP_000420369.1 sporulation sensor histidine kinase KinB -
  MWG54_RS19735 (MWG54_19735) - 3886671..3886871 (+) 201 WP_000929277.1 hypothetical protein -
  MWG54_RS19740 (MWG54_19740) - 3886994..3888157 (+) 1164 WP_000434578.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=676591 MWG54_RS19720 WP_000811494.1 3883637..3884071(-) (nucA/comI) [Bacillus cereus strain SEM-15]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=676591 MWG54_RS19720 WP_000811494.1 3883637..3884071(-) (nucA/comI) [Bacillus cereus strain SEM-15]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGTGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549