Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MRK30_RS17245 Genome accession   NZ_CP095357
Coordinates   3503352..3504152 (-) Length   266 a.a.
NCBI ID   WP_002114699.1    Uniprot ID   -
Organism   Acinetobacter pittii strain 19MO01SH04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3498352..3509152
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRK30_RS17225 (MRK30_17255) - 3499417..3500115 (-) 699 WP_005066180.1 tetratricopeptide repeat protein -
  MRK30_RS17230 (MRK30_17260) hisS 3500144..3501436 (-) 1293 WP_017482039.1 histidine--tRNA ligase -
  MRK30_RS17235 (MRK30_17265) ispG 3501433..3502548 (-) 1116 WP_002115078.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  MRK30_RS17240 (MRK30_17270) - 3502570..3503361 (-) 792 WP_017402542.1 helix-turn-helix domain-containing protein -
  MRK30_RS17245 (MRK30_17275) pilF 3503352..3504152 (-) 801 WP_002114699.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  MRK30_RS17250 (MRK30_17280) rlmN 3504167..3505402 (-) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  MRK30_RS17255 (MRK30_17285) ndk 3505526..3505957 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  MRK30_RS17260 (MRK30_17290) iscX 3506071..3506271 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  MRK30_RS17265 (MRK30_17295) - 3506371..3506922 (-) 552 WP_017482038.1 phosphatase PAP2 family protein -
  MRK30_RS17270 (MRK30_17300) - 3506947..3508236 (-) 1290 WP_080777103.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30114.38 Da        Isoelectric Point: 9.0417

>NTDB_id=676492 MRK30_RS17245 WP_002114699.1 3503352..3504152(-) (pilF) [Acinetobacter pittii strain 19MO01SH04]
MSIPKLKTVLCMGIAVAFLVSGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=676492 MRK30_RS17245 WP_002114699.1 3503352..3504152(-) (pilF) [Acinetobacter pittii strain 19MO01SH04]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCAGTTGCATTTCTGGTAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCAGAGAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAAAGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATCCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCTTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.985

100

0.94
  pilF Acinetobacter baylyi ADP1

76.154

97.744

0.744