Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilW   Type   Machinery gene
Locus tag   MRK30_RS01705 Genome accession   NZ_CP095357
Coordinates   352027..353028 (+) Length   333 a.a.
NCBI ID   WP_032057268.1    Uniprot ID   A0AAE9M9R4
Organism   Acinetobacter pittii strain 19MO01SH04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 347027..358028
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRK30_RS01675 (MRK30_01680) - 348583..348738 (-) 156 Protein_321 hypothetical protein -
  MRK30_RS01680 (MRK30_01685) rpoZ 348777..349055 (-) 279 WP_002116990.1 DNA-directed RNA polymerase subunit omega -
  MRK30_RS01685 (MRK30_01690) gmk 349128..349757 (-) 630 WP_005804544.1 guanylate kinase -
  MRK30_RS01690 (MRK30_01695) ispH 349880..350830 (+) 951 WP_002116992.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  MRK30_RS01695 (MRK30_01700) fimU 351000..351473 (+) 474 WP_032057265.1 GspH/FimT family pseudopilin Machinery gene
  MRK30_RS01700 (MRK30_01705) pilV 351467..352027 (+) 561 WP_080777128.1 type IV pilus modification protein PilV Machinery gene
  MRK30_RS01705 (MRK30_01710) pilW 352027..353028 (+) 1002 WP_032057268.1 PilW family protein Machinery gene
  MRK30_RS01710 (MRK30_01715) pilX 353025..353834 (+) 810 WP_005067126.1 PilX N-terminal domain-containing pilus assembly protein Machinery gene
  MRK30_RS01715 (MRK30_01720) pilY1 353846..357700 (+) 3855 WP_032057269.1 PilC/PilY family type IV pilus protein Machinery gene

Sequence

Protein


Download         Length: 333 a.a.        Molecular weight: 35786.44 Da        Isoelectric Point: 6.6395

>NTDB_id=676469 MRK30_RS01705 WP_032057268.1 352027..353028(+) (pilW) [Acinetobacter pittii strain 19MO01SH04]
MAHSFVSWKQRGFTLIELMVALALGLILVAAATQLFIGGLLSSRLQKANAEIQDSGIFGLEYIARDIRLLNYGNVTNPIL
TDTTPWGGVVLTGSTATNNNNVNFVPNVGTNTYIADTLLSRGAGDTVSTVSNHWKGLSNIQNSSNAVVQSDQLTIQFIAP
TNMTNCEGVNVLAGDLIVERYFLRLDSNGSSQQDYALACDANTPSAVATAQPTTIAGLGDAGQIVLPRIDHFHVLFGAKN
AAGNFAYYTIPQYRAAAQAARDATPSVMPPRILSIQISVLARSTNNAQSKAIDPEQSFFMLDQTVHASDNTTRFLRRTYN
LTIALRNAMGESL

Nucleotide


Download         Length: 1002 bp        

>NTDB_id=676469 MRK30_RS01705 WP_032057268.1 352027..353028(+) (pilW) [Acinetobacter pittii strain 19MO01SH04]
ATGGCTCACTCATTTGTATCGTGGAAACAACGAGGCTTTACATTAATTGAGCTGATGGTGGCATTGGCTTTAGGTCTAAT
TTTGGTGGCCGCGGCGACTCAATTATTTATTGGTGGACTTCTATCAAGTCGTCTACAAAAAGCAAATGCTGAAATACAAG
ATAGCGGTATTTTTGGATTGGAATATATTGCCCGAGATATACGCCTTCTAAATTATGGGAATGTAACCAATCCAATATTA
ACTGATACAACCCCTTGGGGTGGTGTTGTATTAACTGGCTCAACTGCAACCAATAATAACAATGTTAATTTTGTCCCTAA
CGTTGGAACAAATACCTATATTGCTGACACATTACTCAGTCGTGGTGCCGGCGATACGGTGAGTACCGTGAGTAATCATT
GGAAAGGTCTTTCAAATATACAGAACAGTTCAAATGCAGTTGTGCAAAGTGATCAATTGACTATCCAATTTATTGCGCCT
ACGAATATGACCAATTGTGAAGGTGTGAATGTACTTGCAGGTGATTTAATTGTAGAGCGTTATTTTTTACGTCTGGATAG
TAATGGTAGTTCCCAGCAAGATTATGCTCTTGCATGTGATGCAAATACTCCCTCAGCTGTAGCCACAGCTCAACCGACTA
CTATTGCTGGGTTGGGCGACGCTGGTCAAATTGTATTGCCGCGTATTGATCATTTCCATGTGCTGTTTGGTGCAAAAAAT
GCGGCTGGCAATTTTGCTTATTATACCATTCCCCAATATCGCGCTGCTGCACAGGCTGCTCGAGATGCTACACCATCTGT
GATGCCGCCACGAATTCTTTCAATACAAATATCTGTATTAGCGCGCTCTACTAATAATGCCCAAAGCAAAGCAATAGATC
CTGAGCAATCTTTTTTTATGTTAGATCAAACCGTGCATGCTTCTGATAATACAACTCGATTCTTACGCCGCACTTATAAC
TTAACAATTGCTCTACGCAACGCGATGGGGGAAAGTCTATGA

Domains

Predicted by InterproScan.

Type IV Pilus-assembly protein W (PilW)

(181-327)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilW Acinetobacter baumannii D1279779

85.586

100

0.856