Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MRL80_RS00170 Genome accession   NZ_CP095333
Coordinates   40471..41841 (+) Length   456 a.a.
NCBI ID   WP_131639199.1    Uniprot ID   -
Organism   Enterococcus faecalis strain 19CA02SA02     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 35471..46841
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRL80_RS00145 (MRL80_00145) - 35961..36428 (-) 468 WP_002397711.1 VOC family protein -
  MRL80_RS00150 (MRL80_00150) - 36493..37440 (+) 948 WP_002389777.1 YafY family protein -
  MRL80_RS00155 (MRL80_00155) - 37532..38785 (-) 1254 WP_002404712.1 glutamate-5-semialdehyde dehydrogenase -
  MRL80_RS00160 (MRL80_00160) proB 38772..39587 (-) 816 WP_002389550.1 glutamate 5-kinase -
  MRL80_RS00165 (MRL80_00165) - 39907..40392 (+) 486 WP_002361472.1 dUTP diphosphatase -
  MRL80_RS00170 (MRL80_00170) radA 40471..41841 (+) 1371 WP_131639199.1 DNA repair protein RadA Machinery gene
  MRL80_RS00175 (MRL80_00175) - 41942..43087 (+) 1146 WP_002368622.1 PIN/TRAM domain-containing protein -
  MRL80_RS00180 (MRL80_00180) ispF 43106..43579 (+) 474 WP_002367570.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  MRL80_RS00185 (MRL80_00185) gltX 43632..45089 (+) 1458 WP_131639200.1 glutamate--tRNA ligase -
  MRL80_RS00190 (MRL80_00190) epsC 45359..45898 (+) 540 WP_002356054.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49784.35 Da        Isoelectric Point: 7.7030

>NTDB_id=676379 MRL80_RS00170 WP_131639199.1 40471..41841(+) (radA) [Enterococcus faecalis strain 19CA02SA02]
MAKKAKVQFECQSCGYVSPKYLGRCPNCGQWNSMVEEVIQDTSDRRARVSLTGKKTQPQRLSEVIPKKEPRVKTELVELN
RVLGGGVVPGSLILIGGDPGIGKSTLLLQVSQQLAATGGTVLYVSGEESAEQIKLRAERLGTVNETFYLYAETDMHEISR
AIEKLEPDYVIIDSIQTMTQPDVTSVAGSVSQVRETTAELLKLAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GDKHHTFRILRAVKNRFGSTNEIGIFEMQTHGLVEVMNPSQVFLEERLEGATGSSIVVAMEGSRPILVEIQALVTPTMFG
NAKRTTTGLNFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKINEPAIDLALAISIASSYKEKGTSSSECFIGEIGLTG
EIRRVNSIEQRVREAQKLGFTKVYVPKNNLGGWEAPEGIEIIGVSTIGETLRKVFK

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=676379 MRL80_RS00170 WP_131639199.1 40471..41841(+) (radA) [Enterococcus faecalis strain 19CA02SA02]
ATGGCAAAAAAAGCAAAAGTTCAATTTGAATGTCAAAGCTGTGGGTATGTGTCTCCAAAATATCTCGGCCGCTGTCCTAA
TTGTGGACAATGGAATTCGATGGTGGAAGAGGTTATTCAAGATACTTCTGATCGTCGTGCTAGAGTAAGTTTGACTGGTA
AAAAGACACAACCACAACGGCTATCTGAAGTCATACCTAAAAAAGAGCCTCGTGTAAAAACTGAATTAGTGGAGCTGAAT
CGGGTTTTAGGTGGTGGCGTTGTTCCAGGTTCGTTAATTTTAATCGGTGGCGATCCCGGAATTGGTAAGTCAACATTGCT
CTTACAAGTATCGCAACAATTAGCAGCCACAGGTGGTACGGTTTTATATGTTTCTGGTGAAGAAAGTGCTGAGCAAATTA
AATTGCGAGCGGAGCGTTTAGGCACCGTCAACGAGACATTTTACTTATACGCAGAAACAGATATGCACGAAATTTCGCGA
GCCATTGAAAAATTAGAACCAGACTATGTCATTATTGACTCGATTCAAACAATGACGCAGCCAGATGTTACCAGCGTTGC
TGGTAGTGTCAGTCAAGTCCGAGAAACAACTGCTGAATTATTGAAACTGGCAAAAACGAATGGGATTGCCATTTTTATTG
TCGGACACGTAACGAAGGAAGGGTCTATCGCAGGGCCACGGATGTTGGAACATATGGTAGACACGGTTCTTTATTTTGAA
GGAGATAAGCATCACACTTTTAGAATCTTACGGGCTGTCAAAAATCGGTTTGGCTCAACGAATGAGATTGGTATTTTTGA
AATGCAGACACATGGATTGGTTGAAGTTATGAATCCTTCTCAGGTCTTTTTAGAAGAACGTTTAGAAGGAGCCACCGGAT
CTTCAATTGTGGTAGCAATGGAAGGTTCACGCCCCATTTTGGTGGAAATCCAAGCGTTAGTTACCCCAACGATGTTTGGC
AATGCCAAGCGAACGACCACCGGGTTGAACTTTAATCGCGTTTCTTTAATCATGGCGGTTTTAGAAAAACGGGCAGGCCT
TTTATTACAAAACCAGGATGCGTATCTAAAAGCGGCTGGTGGAGTCAAAATTAATGAACCAGCTATTGATTTAGCGTTAG
CAATCAGTATTGCTTCTAGCTATAAAGAAAAAGGAACCTCATCTTCGGAATGTTTCATTGGTGAGATTGGTTTAACTGGT
GAAATTCGCCGCGTGAATAGTATTGAACAACGAGTGCGTGAAGCGCAAAAACTAGGCTTTACAAAAGTCTATGTCCCTAA
AAATAACCTTGGTGGCTGGGAAGCGCCTGAAGGGATTGAAATTATTGGCGTTTCAACAATTGGTGAAACGTTAAGAAAAG
TTTTTAAATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(71-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.945

99.781

0.748
  radA Streptococcus pneumoniae Rx1

74.945

99.781

0.748
  radA Streptococcus pneumoniae D39

74.945

99.781

0.748
  radA Streptococcus pneumoniae R6

74.945

99.781

0.748
  radA Streptococcus pneumoniae TIGR4

74.945

99.781

0.748
  radA Streptococcus mitis SK321

74.945

99.781

0.748
  radA Bacillus subtilis subsp. subtilis str. 168

65.854

98.904

0.651