Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MRK42_RS19710 Genome accession   NZ_CP095328
Coordinates   4276020..4277459 (-) Length   479 a.a.
NCBI ID   WP_042070883.1    Uniprot ID   A0AAU6T7T1
Organism   Aeromonas sp. 19NY04SH05-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4271020..4282459
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRK42_RS19680 (MRK42_19625) - 4271380..4272741 (+) 1362 WP_026456105.1 Do family serine endopeptidase -
  MRK42_RS19685 (MRK42_19630) degS 4272832..4273935 (+) 1104 WP_354688568.1 outer membrane-stress sensor serine endopeptidase DegS -
  MRK42_RS19690 (MRK42_19635) npr 4274057..4274329 (-) 273 WP_026456107.1 PTS phosphocarrier protein NPr -
  MRK42_RS19695 (MRK42_19640) rapZ 4274350..4275216 (-) 867 WP_042070881.1 RNase adapter RapZ -
  MRK42_RS19700 (MRK42_19645) ptsN 4275262..4275708 (-) 447 WP_005336322.1 PTS IIA-like nitrogen regulatory protein PtsN -
  MRK42_RS19705 (MRK42_19650) hpf 4275711..4275998 (-) 288 WP_005336323.1 ribosome hibernation promoting factor -
  MRK42_RS19710 (MRK42_19655) rpoN/rpoN1 4276020..4277459 (-) 1440 WP_042070883.1 RNA polymerase factor sigma-54 Machinery gene
  MRK42_RS19715 (MRK42_19660) lptB 4277525..4278250 (-) 726 WP_026456111.1 LPS export ABC transporter ATP-binding protein -
  MRK42_RS19720 (MRK42_19665) lptA 4278254..4278781 (-) 528 WP_026456112.1 lipopolysaccharide transport periplasmic protein LptA -
  MRK42_RS19725 (MRK42_19670) lptC 4278768..4279328 (-) 561 WP_026456113.1 LPS export ABC transporter periplasmic protein LptC -
  MRK42_RS19730 (MRK42_19675) kdsC 4279325..4279879 (-) 555 WP_124239993.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  MRK42_RS19735 (MRK42_19680) - 4279879..4280874 (-) 996 WP_216993544.1 KpsF/GutQ family sugar-phosphate isomerase -
  MRK42_RS19740 (MRK42_19685) mlaF 4281082..4281882 (+) 801 WP_354688569.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 54020.83 Da        Isoelectric Point: 4.3230

>NTDB_id=676372 MRK42_RS19710 WP_042070883.1 4276020..4277459(-) (rpoN/rpoN1) [Aeromonas sp. 19NY04SH05-1]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSSLELQQEIQQALENNPLLEQEESEVQETSPQDQVDASQLDSSDAMAQDQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGDDTVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVSLED
IQAAVSSDEVEVELDEIEVVLKRVQHFDPVGIAARSVQECLLIQLEQYDPETPWISEAKEVIQEHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKRQNRWVVELNPDSSPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=676372 MRK42_RS19710 WP_042070883.1 4276020..4277459(-) (rpoN/rpoN1) [Aeromonas sp. 19NY04SH05-1]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAATCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCTCTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAACAATCCCCTGCTGGAGCAGGAAGAGAGTGAGG
TGCAGGAGACATCCCCGCAAGATCAGGTTGATGCCAGCCAGCTGGATTCCAGCGATGCCATGGCCCAGGATCAGATGCCG
GACGAGTTGCCGGTGGATACGACCTGGGACGAGGTCTATTCCGCGGGGACGGCCCAGAGTGCCGGACCCATCCATGATGG
GGATGACACCGTCTACCAGGGGGAGACCACCGAGAACCTGCAGGACTACCTGTTGTGGCAGATGCGCCTCACCCCGTTCA
GCGATGTGGATGCCGCCATCGCGCTTGCCATCATAGATGCCATCGACGAATCCGGTTATCTGACAGTGTCGCTGGAAGAT
ATCCAAGCTGCGGTGAGCAGCGACGAAGTGGAGGTCGAACTCGATGAAATCGAGGTCGTGCTCAAGCGGGTGCAACACTT
CGATCCGGTGGGAATTGCCGCCCGTTCGGTGCAGGAGTGCCTGCTGATCCAGCTGGAACAGTACGACCCCGAGACTCCCT
GGATCAGTGAGGCCAAGGAGGTGATCCAGGAGCACATGGATCTGCTTGGCAATCGCGATTACCGCACCCTGGCCCGCAAG
ACCCGGCTCAAGGAGAGCGACCTCAAGGAGGTGCTGGATCTGATCCAGCTGTTGGAGCCACGGCCGGGCAACGGCATCAT
CCAGAGCGACTCCCAGTACGTGATCCCGGACGTCTCCGTAGTCAAACGGCAGAATCGCTGGGTGGTGGAACTCAATCCCG
ATTCATCCCCGCGCATCCGGGTCAACGAGACCTATGCCGCTATGGCACGTAACGCCCGTAGCAGCACGGATACCCAGTTT
ATCCGCTCCCACTTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTTGAGAGCCGCAACGACACTCTGTTGAAAGTGGCCAG
CTGCATCGTCGAGCAGCAACAGGGCTTTTTCGAATATGGTGAAGAGGCGATGAAGCCGATGGTGCTCAACGATATCGCCG
AAGCGGTGGAGATGCACGAGTCGACCATCTCCCGTGTAACAACCCAGAAATACATGCATACCCCCCGTGGTATTTTCGAA
CTGAAGTTTTTTTTCTCCAGCCATGTGAATACAGAAGGAGGAGGAGAGTGCTCATCGACCGCAATCCGTGCGCTCATCAA
GAAGCTGGTGACGGCGGAGAACCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCTAGACGCACGATCGCTAAGTACCGCGAATCCCTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.882

100

0.489