Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MRN59_RS00570 Genome accession   NZ_CP095314
Coordinates   97828..99189 (+) Length   453 a.a.
NCBI ID   WP_015912383.1    Uniprot ID   B9E8S3
Organism   Macrococcoides caseolyticum strain 19MO06SA05     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 92828..104189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRN59_RS00550 (MRN59_00550) - 93317..93778 (+) 462 WP_101141334.1 CtsR family transcriptional regulator -
  MRN59_RS00555 (MRN59_00555) - 93789..94175 (+) 387 WP_354680366.1 UvrB/UvrC motif-containing protein -
  MRN59_RS00560 (MRN59_00560) - 94165..95169 (+) 1005 WP_133446051.1 protein arginine kinase -
  MRN59_RS00565 (MRN59_00565) - 95179..97605 (+) 2427 WP_101141329.1 ATP-dependent Clp protease ATP-binding subunit -
  MRN59_RS00570 (MRN59_00570) radA 97828..99189 (+) 1362 WP_015912383.1 DNA repair protein RadA Machinery gene
  MRN59_RS00575 (MRN59_00575) - 99202..100251 (+) 1050 WP_015912382.1 PIN/TRAM domain-containing protein -
  MRN59_RS00580 (MRN59_00580) ispD 100295..100924 (+) 630 WP_354680367.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MRN59_RS00585 (MRN59_00585) ispF 100921..101400 (+) 480 WP_354680368.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  MRN59_RS00590 (MRN59_00590) gltX 101418..102872 (+) 1455 WP_133446054.1 glutamate--tRNA ligase -
  MRN59_RS00595 (MRN59_00595) cysE 103109..103762 (+) 654 WP_101038220.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 50240.85 Da        Isoelectric Point: 7.2379

>NTDB_id=676318 MRN59_RS00570 WP_015912383.1 97828..99189(+) (radA) [Macrococcoides caseolyticum strain 19MO06SA05]
MAKVKSTFECMACGYQSPKWMGKCPNCGAWNQMEEVIEHKQKGPKNAISESQSDNKVEKLKDITKESVPRDHTQMKELDR
VLGGGIVPGSLILIGGDPGIGKSTLLLQVCAMLSQNHPVLYISGEESVRQTKLRADRLLEDAGELDVYAETNLQIIHETV
KRSKPKFLVIDSIQTIFHPEVTSAPGSVSQVRECTQELMRIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
THHSYRILRAVKNRFGSTNEMGIFEMKNTGLKEVLNPSEMFLEERTKNVAGSTIVATMEGTRPLLVEVQSLVTPTSFHNP
RRMASGVDHNRLNLLMAVLEKKQGYLLQQQDAYVKVAGGVKLDEPAVDLSVIVSIASSYNDKPTRGDDCFIGEVGLTGEV
RRVARIEQRVQEAEKLGFKRVIIPKNNIGGWDFPGNIEVIGVTNINEALKMAF

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=676318 MRN59_RS00570 WP_015912383.1 97828..99189(+) (radA) [Macrococcoides caseolyticum strain 19MO06SA05]
TTGGCAAAGGTGAAAAGTACATTTGAATGTATGGCCTGTGGTTATCAGTCACCGAAATGGATGGGAAAATGTCCGAATTG
TGGTGCATGGAATCAAATGGAAGAAGTTATTGAACATAAACAGAAGGGTCCTAAAAATGCGATTTCTGAATCACAAAGTG
ATAATAAAGTTGAAAAACTAAAAGATATAACGAAAGAAAGTGTTCCTCGTGATCATACGCAGATGAAAGAACTAGATCGT
GTACTTGGAGGAGGTATCGTTCCCGGTTCATTGATACTTATCGGTGGAGACCCAGGGATTGGGAAATCCACATTACTACT
ACAAGTTTGTGCAATGCTTTCACAAAACCATCCCGTACTTTATATTTCTGGAGAGGAATCGGTAAGACAAACGAAACTAC
GTGCCGATCGTCTATTAGAGGATGCAGGGGAGCTTGATGTCTATGCAGAAACCAACCTTCAGATTATTCACGAAACGGTA
AAACGATCGAAACCGAAATTTTTAGTTATCGATTCAATTCAAACAATATTTCATCCAGAGGTTACAAGTGCACCTGGTTC
TGTATCGCAAGTTAGAGAATGTACACAAGAGTTAATGCGTATTGCAAAGCAGATGAACATTGCAACATTTATCGTAGGGC
ACGTAACAAAAGAAGGACAAATTGCAGGTCCAAGGCTACTAGAACATATGGTTGATACAGTTCTTTACTTTGAAGGAGAC
ACTCATCACAGCTACAGAATATTACGCGCGGTGAAGAATCGTTTTGGTTCGACAAATGAAATGGGTATATTTGAAATGAA
GAATACAGGCCTTAAAGAAGTGCTGAACCCTTCAGAAATGTTCCTTGAAGAACGTACAAAAAATGTAGCGGGTTCAACTA
TCGTTGCAACGATGGAAGGCACGCGTCCATTACTAGTCGAAGTTCAGTCACTTGTAACGCCGACATCCTTTCATAATCCA
AGAAGAATGGCATCAGGTGTGGATCATAATCGTCTGAATTTACTGATGGCTGTACTAGAGAAAAAACAAGGATATTTATT
ACAGCAGCAGGATGCATATGTGAAGGTTGCGGGCGGTGTCAAACTTGATGAACCTGCTGTTGACTTAAGTGTAATCGTAA
GCATTGCTTCAAGCTATAACGATAAGCCGACACGAGGAGATGACTGTTTTATCGGAGAAGTCGGTCTGACTGGTGAAGTG
AGACGTGTTGCGCGTATTGAACAACGTGTACAAGAAGCTGAAAAACTCGGTTTTAAACGTGTTATCATCCCCAAAAATAA
TATTGGCGGCTGGGATTTCCCTGGAAATATAGAAGTAATCGGTGTAACTAATATTAACGAAGCATTAAAAATGGCATTTT
AA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(343-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(66-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B9E8S3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.447

100

0.669
  radA Streptococcus pneumoniae Rx1

62.996

100

0.631
  radA Streptococcus pneumoniae D39

62.996

100

0.631
  radA Streptococcus pneumoniae R6

62.996

100

0.631
  radA Streptococcus pneumoniae TIGR4

62.996

100

0.631
  radA Streptococcus mitis NCTC 12261

62.996

100

0.631
  radA Streptococcus mitis SK321

62.996

100

0.631