Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   GR930_RS21010 Genome accession   NZ_AP022351
Coordinates   4329463..4330140 (+) Length   225 a.a.
NCBI ID   WP_000178170.1    Uniprot ID   -
Organism   Escherichia coli strain E138     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4324463..4335140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GR930_RS20975 (RIMDOsaka138_40460) gspG 4324943..4325380 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  GR930_RS20980 (RIMDOsaka138_40470) gspH 4325388..4325897 (+) 510 WP_001076039.1 type II secretion system minor pseudopilin GspH -
  GR930_RS20985 (RIMDOsaka138_40480) gspI 4325894..4326271 (+) 378 WP_001041749.1 type II secretion system minor pseudopilin GspI -
  GR930_RS20990 (RIMDOsaka138_40490) gspJ 4326264..4326851 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  GR930_RS20995 (RIMDOsaka138_40500) gspK 4326844..4327827 (+) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  GR930_RS21000 (RIMDOsaka138_40510) gspL 4327842..4329005 (+) 1164 WP_001296464.1 type II secretion system protein GspL -
  GR930_RS21005 (RIMDOsaka138_40520) gspM 4329002..4329463 (+) 462 WP_001296465.1 type II secretion system protein GspM -
  GR930_RS21010 (RIMDOsaka138_40530) pilD 4329463..4330140 (+) 678 WP_000178170.1 prepilin peptidase GspO Machinery gene
  GR930_RS21015 (RIMDOsaka138_40540) bfr 4330169..4330645 (-) 477 WP_000675504.1 bacterioferritin -
  GR930_RS21020 (RIMDOsaka138_40550) bfd 4330717..4330911 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  GR930_RS21025 (RIMDOsaka138_40560) chiA 4331080..4333782 (-) 2703 WP_000773180.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24842.69 Da        Isoelectric Point: 7.6364

>NTDB_id=67630 GR930_RS21010 WP_000178170.1 4329463..4330140(+) (pilD) [Escherichia coli strain E138]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=67630 GR930_RS21010 WP_000178170.1 4329463..4330140(+) (pilD) [Escherichia coli strain E138]
ATGACAATGTTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.95

100

0.382

Multiple sequence alignment