Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NOS3756_RS22545 Genome accession   NZ_AP017295
Coordinates   5435776..5436882 (+) Length   368 a.a.
NCBI ID   WP_067776100.1    Uniprot ID   -
Organism   Nostoc sp. NIES-3756     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5430776..5441882
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NOS3756_RS22530 (NOS3756_46480) - 5431197..5433299 (-) 2103 WP_067772931.1 S8 family serine peptidase -
  NOS3756_RS22535 (NOS3756_46490) - 5433476..5434051 (+) 576 WP_067772934.1 hypothetical protein -
  NOS3756_RS22540 (NOS3756_46500) - 5434256..5435554 (-) 1299 WP_067776097.1 sugar ABC transporter substrate-binding protein -
  NOS3756_RS22545 (NOS3756_46510) pilM 5435776..5436882 (+) 1107 WP_067776100.1 type IV pilus biogenesis protein PilM Machinery gene
  NOS3756_RS22550 (NOS3756_46520) - 5437005..5437772 (+) 768 WP_067772937.1 PilN domain-containing protein -
  NOS3756_RS22555 (NOS3756_46530) - 5437769..5438536 (+) 768 WP_067772940.1 pilus assembly protein PilO -
  NOS3756_RS22560 (NOS3756_46540) - 5438832..5441348 (+) 2517 WP_067772943.1 type IV pilus secretin family protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40521.41 Da        Isoelectric Point: 4.4320

>NTDB_id=67594 NOS3756_RS22545 WP_067776100.1 5435776..5436882(+) (pilM) [Nostoc sp. NIES-3756]
MVKSLSSLFGKSHRGVGVEIAPERVNVVQLRKQRQGLKLESLTSVPVPEGIVSDGQITDPPAMAQLIQQALAESKIKVSS
VATGVPGRDAIVRIIPVPAELDDKELRDMVLNHEAGLYLPYPREEADVDYQKLGYFVDEDGIEKVQVLLVATRKEVTDTY
IRTFEQAGLQISVLEINSFALIRTIRDQLRQFGPQEATVLVDIEFDSTEIAIIINGVPQFSRTVPIGTYQMQMAYARAMS
LPTSRDMEILQEMVIPVNSGDGDRTGMTETDPAMAGVLRILGELTDELRRSIDFYLNQSENLEIVQILLGGPGGGLQQLN
EFFTQRLSIPTNQIDPIGSLSLQVDEAKYPSTQRSGLGIVLGLGMREV

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=67594 NOS3756_RS22545 WP_067776100.1 5435776..5436882(+) (pilM) [Nostoc sp. NIES-3756]
GTGGTGAAAAGTCTCAGTAGTCTGTTTGGAAAATCTCATAGAGGAGTGGGTGTAGAAATTGCACCAGAACGGGTGAATGT
AGTTCAACTACGCAAGCAGCGCCAAGGCTTGAAACTCGAATCTTTAACATCGGTTCCAGTTCCCGAAGGAATTGTCAGTG
ATGGTCAAATCACTGATCCCCCAGCAATGGCGCAATTAATTCAGCAGGCGCTGGCTGAAAGCAAAATCAAAGTATCTAGT
GTAGCAACTGGTGTTCCTGGAAGGGATGCGATCGTCCGAATTATCCCTGTACCGGCAGAGTTGGATGATAAAGAATTACG
GGATATGGTTTTAAACCACGAAGCAGGCTTATATTTGCCTTATCCCCGTGAGGAAGCTGATGTAGATTATCAAAAACTTG
GGTACTTTGTAGATGAGGACGGCATTGAGAAGGTACAAGTACTTTTAGTTGCTACTCGCAAGGAGGTGACAGATACATAT
ATTAGAACTTTTGAGCAAGCAGGTTTACAAATTAGCGTTTTGGAAATCAACAGTTTTGCTTTAATTCGGACAATTCGTGA
TCAACTACGACAATTTGGGCCACAAGAAGCAACCGTACTAGTTGACATAGAGTTCGACAGTACAGAAATTGCCATCATTA
TTAACGGAGTTCCCCAATTTTCCCGTACTGTCCCCATTGGCACATATCAAATGCAAATGGCTTATGCCAGAGCAATGAGT
TTGCCTACATCACGAGACATGGAAATTTTACAGGAAATGGTAATTCCTGTAAATTCTGGTGATGGTGATAGAACTGGGAT
GACAGAAACTGACCCGGCTATGGCTGGTGTGTTGCGTATCTTGGGAGAACTAACAGATGAGTTACGCCGTTCTATAGATT
TTTACTTAAATCAAAGTGAAAACTTAGAAATCGTCCAGATTCTATTAGGTGGGCCTGGTGGTGGATTACAGCAACTTAAT
GAGTTTTTTACTCAACGCTTAAGTATTCCTACTAATCAAATAGATCCTATCGGGTCTTTGTCCTTGCAAGTTGATGAAGC
AAAGTATCCCTCTACACAACGTTCTGGGTTAGGAATAGTCCTTGGTTTAGGGATGCGGGAGGTATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-234)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

65.668

99.728

0.655

Multiple sequence alignment