Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MUA12_RS01470 Genome accession   NZ_CP095104
Coordinates   294425..295795 (+) Length   456 a.a.
NCBI ID   WP_002481315.1    Uniprot ID   -
Organism   Staphylococcus simulans strain IVB6179     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 289425..300795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUA12_RS01450 (MUA12_01425) - 289667..290128 (+) 462 WP_262577848.1 CtsR family transcriptional regulator -
  MUA12_RS01455 (MUA12_01430) - 290248..290652 (+) 405 Protein_270 UvrB/UvrC motif-containing protein -
  MUA12_RS01460 (MUA12_01435) - 290656..291660 (+) 1005 WP_262577849.1 protein arginine kinase -
  MUA12_RS01465 (MUA12_01440) - 291672..294137 (+) 2466 WP_262577850.1 ATP-dependent Clp protease ATP-binding subunit -
  MUA12_RS01470 (MUA12_01445) radA 294425..295795 (+) 1371 WP_002481315.1 DNA repair protein RadA Machinery gene
  MUA12_RS01475 (MUA12_01450) - 295823..296887 (+) 1065 WP_262577851.1 PIN/TRAM domain-containing protein -
  MUA12_RS01480 (MUA12_01455) gltX 297174..298628 (+) 1455 WP_262577852.1 glutamate--tRNA ligase -
  MUA12_RS01485 (MUA12_01460) cysE 298928..299557 (+) 630 WP_172954255.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50252.78 Da        Isoelectric Point: 7.4601

>NTDB_id=675452 MUA12_RS01470 WP_002481315.1 294425..295795(+) (radA) [Staphylococcus simulans strain IVB6179]
MAKKKVTFECMACGYQSPKWMGKCPNCGAWNQMEEVMTQKEAPSGRGMRTREQTAKVTKLNDVQHESTPRIHTSSREFDR
VLGGGIVQGSLVLIGGDPGIGKSTLLLQICSALSQNKKVLYITGEESLNQTKLRADRLEEDSSQLNVFAETDLEVIKEAV
KQTQPDLVVVDSIQTVYHPEISSAPGSVSQVRESTQILMGIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
EHHAYRILRAVKNRFGSTNEMGIFEMKHSGLKGVKNPSEMFLEERSTNVAGSTIVPTMEGTRPLLIEVQALVTPTTFNNP
RRMATGIDHNRLNLLMAVLEKKESYLLQQQDAYVKVAGGVRLTEPAVDLAIIAAVASSFKDQPINGMDCFVGEVGLTGEV
RRVSRIEQRVQEAAKLGFKRVIIPKTNIGGWDFPDNIQVIGVTSVHEALNFALMKS

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=675452 MUA12_RS01470 WP_002481315.1 294425..295795(+) (radA) [Staphylococcus simulans strain IVB6179]
TTGGCAAAAAAGAAAGTGACATTTGAATGTATGGCATGTGGTTATCAATCACCGAAATGGATGGGGAAATGTCCGAATTG
TGGTGCTTGGAACCAAATGGAAGAAGTTATGACGCAAAAAGAAGCTCCAAGTGGTAGAGGGATGCGTACAAGAGAACAAA
CAGCTAAAGTAACAAAGTTAAATGATGTCCAACATGAATCTACACCGCGTATCCATACAAGTTCGAGAGAATTTGATCGT
GTCTTAGGTGGCGGTATTGTCCAAGGTTCGCTCGTGTTAATCGGTGGGGACCCTGGTATTGGTAAATCGACATTATTACT
TCAAATTTGTTCTGCGTTATCTCAAAATAAAAAAGTACTTTATATTACAGGTGAGGAATCACTGAATCAAACGAAATTGC
GAGCAGATCGTTTAGAAGAAGATTCAAGTCAATTAAACGTCTTTGCGGAAACAGATTTAGAAGTTATTAAAGAAGCTGTT
AAACAAACACAACCAGACTTAGTGGTAGTAGACTCGATTCAAACAGTGTATCATCCGGAAATCAGTTCAGCGCCTGGTTC
TGTCTCACAAGTACGAGAAAGTACGCAAATTCTGATGGGGATTGCGAAACAAATGAATATCGCAACCTTTATCGTCGGTC
ATGTGACGAAAGAAGGACAAATTGCAGGTCCACGTTTATTAGAACATATGGTAGATACGGTACTATATTTCGAAGGGGAC
GAACATCATGCATATAGAATTCTGCGTGCAGTGAAGAACCGTTTCGGTTCAACGAATGAAATGGGAATCTTCGAAATGAA
ACATAGCGGTTTGAAAGGAGTTAAGAACCCTTCAGAGATGTTCTTAGAAGAACGTTCAACGAATGTAGCTGGCTCTACAA
TTGTTCCGACAATGGAAGGTACACGTCCTTTATTAATTGAAGTGCAAGCATTAGTGACACCGACAACATTTAATAACCCA
AGACGTATGGCGACTGGTATCGATCATAACCGTTTAAATTTATTAATGGCAGTGTTAGAGAAGAAAGAAAGTTACTTGCT
GCAACAACAAGATGCTTATGTCAAAGTTGCAGGCGGCGTGCGTTTAACAGAACCAGCTGTTGATTTAGCAATTATCGCAG
CGGTTGCTTCTAGTTTTAAAGACCAACCTATCAACGGCATGGATTGTTTCGTCGGTGAAGTAGGTCTGACAGGAGAAGTG
CGACGTGTCTCACGTATTGAACAACGTGTTCAAGAAGCAGCGAAATTAGGTTTCAAACGTGTGATTATTCCTAAAACCAA
TATAGGAGGTTGGGACTTCCCAGATAATATTCAAGTTATCGGTGTAACCAGTGTGCATGAAGCACTGAACTTTGCATTGA
TGAAAAGCTGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-427)

AAA domain (AAA_25)

(69-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.864

100

0.66
  radA Streptococcus pneumoniae Rx1

60.802

98.465

0.599
  radA Streptococcus pneumoniae D39

60.802

98.465

0.599
  radA Streptococcus pneumoniae R6

60.802

98.465

0.599
  radA Streptococcus pneumoniae TIGR4

60.802

98.465

0.599
  radA Streptococcus mitis NCTC 12261

60.802

98.465

0.599
  radA Streptococcus mitis SK321

60.802

98.465

0.599