Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MUO14_RS12385 Genome accession   NZ_CP095074
Coordinates   2509041..2510417 (+) Length   458 a.a.
NCBI ID   WP_244751011.1    Uniprot ID   -
Organism   Halobacillus shinanisalinarum strain SSTM10-2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2504041..2515417
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUO14_RS12365 (MUO14_12365) - 2504287..2504751 (+) 465 WP_244751007.1 CtsR family transcriptional regulator -
  MUO14_RS12370 (MUO14_12370) - 2504772..2505320 (+) 549 WP_244751008.1 UvrB/UvrC motif-containing protein -
  MUO14_RS12375 (MUO14_12375) - 2505320..2506390 (+) 1071 WP_244751009.1 protein arginine kinase -
  MUO14_RS12380 (MUO14_12380) clpC 2506410..2508866 (+) 2457 WP_244751010.1 ATP-dependent protease ATP-binding subunit ClpC -
  MUO14_RS12385 (MUO14_12385) radA 2509041..2510417 (+) 1377 WP_244751011.1 DNA repair protein RadA Machinery gene
  MUO14_RS12390 (MUO14_12390) disA 2510418..2511491 (+) 1074 WP_244751012.1 DNA integrity scanning diadenylate cyclase DisA -
  MUO14_RS12395 (MUO14_12395) - 2511646..2512755 (+) 1110 WP_244751013.1 PIN/TRAM domain-containing protein -
  MUO14_RS12400 (MUO14_12400) - 2512914..2514104 (+) 1191 WP_255822111.1 bifunctional 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50010.51 Da        Isoelectric Point: 7.4569

>NTDB_id=675016 MUO14_RS12385 WP_244751011.1 2509041..2510417(+) (radA) [Halobacillus shinanisalinarum strain SSTM10-2]
MAKRKTKFVCQECGYETPKWMGKCPGCHQWNTLVEEVSATASNSRHVFQTNSSSSTKPEKITAIKSEKEPRLATDMPELN
RVLGGGIVAGSLVLIGGDPGIGKSTLLLQVSAQIANKQLPVLYISGEESSRQTKLRAERLGIKSEELYVLPETNLHDVVS
QIEAIDPKFVVIDSIQTIFKEDVTSAPGSVSQVRECTSQLMRIAKSKGIPIFIVGHVTKEGSIAGPRLLEHMVDAVLYFE
GERHHTFRILRSVKNRFGSTHEMGIFEMKEKGLEEVLNPSEIFLEERSQGAAGSIVVASMEGTRPVLVEIQALISPTSYG
NPRRMATGLDTNRVPLLMAVLEKRAGLLLQNQDAYVKVAGGVKLDEPAIDLGVALSIASSFRDQASNGDDVVVGEVGLTG
EIRRVARVEQRVQEANKLGFKRVIIPKKNLDGWTPPHGIEVIGVNTVQEAMQVTLGER

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=675016 MUO14_RS12385 WP_244751011.1 2509041..2510417(+) (radA) [Halobacillus shinanisalinarum strain SSTM10-2]
TTGGCAAAGAGAAAGACAAAATTTGTTTGTCAGGAATGTGGTTATGAAACGCCAAAATGGATGGGGAAATGTCCGGGCTG
CCACCAGTGGAATACACTAGTGGAGGAGGTATCAGCTACGGCCTCAAACTCTAGACACGTTTTTCAAACCAATTCTTCGT
CATCGACTAAGCCTGAGAAAATTACAGCGATTAAATCAGAGAAAGAACCACGTCTTGCTACAGATATGCCTGAATTAAAC
CGAGTGCTTGGTGGAGGTATTGTTGCTGGATCTTTGGTTTTGATTGGTGGCGATCCGGGTATCGGGAAATCAACATTACT
ACTTCAAGTTTCCGCACAAATTGCTAATAAACAACTCCCGGTTTTGTATATTTCAGGTGAGGAATCCTCTCGTCAAACGA
AGCTGCGTGCTGAACGCCTCGGGATCAAGTCTGAAGAGCTCTATGTACTACCAGAAACTAATTTACATGATGTGGTAAGT
CAAATAGAGGCGATTGATCCTAAGTTTGTAGTGATTGACTCCATTCAAACCATATTTAAGGAGGATGTTACTTCAGCGCC
AGGGAGTGTGTCGCAAGTTCGTGAATGCACAAGCCAGCTGATGAGAATAGCTAAGAGTAAAGGTATTCCAATTTTTATTG
TCGGCCATGTGACAAAAGAGGGCTCAATTGCAGGACCGCGTTTACTAGAACATATGGTCGATGCCGTCCTTTATTTTGAG
GGAGAAAGACATCATACCTTTCGGATTTTACGTAGTGTCAAGAACCGTTTTGGCAGTACACATGAGATGGGAATTTTTGA
AATGAAGGAGAAGGGCCTGGAAGAGGTTCTGAATCCATCGGAGATTTTTCTTGAGGAGAGGTCTCAGGGAGCTGCAGGAT
CGATTGTTGTAGCCTCTATGGAAGGTACAAGGCCTGTATTAGTTGAGATCCAGGCGTTAATTTCCCCGACATCTTATGGA
AACCCTAGAAGGATGGCTACAGGACTTGATACGAACCGCGTGCCTTTATTAATGGCTGTATTAGAAAAGCGGGCAGGTTT
GCTGCTTCAGAATCAGGATGCCTATGTAAAGGTGGCAGGTGGGGTCAAGCTGGATGAACCTGCGATTGATTTGGGAGTAG
CCTTAAGCATTGCTTCAAGCTTCCGTGATCAGGCCTCGAATGGTGATGATGTAGTCGTAGGTGAAGTCGGGTTAACGGGT
GAGATAAGAAGAGTAGCACGTGTTGAACAACGGGTTCAGGAGGCAAACAAGCTCGGTTTTAAACGCGTCATTATTCCAAA
GAAGAACTTAGATGGCTGGACACCGCCACATGGGATTGAGGTAATCGGTGTCAACACTGTCCAAGAAGCTATGCAGGTCA
CATTGGGGGAGCGGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(68-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

72.648

99.782

0.725
  radA Streptococcus mitis SK321

62.168

98.69

0.614
  radA Streptococcus mitis NCTC 12261

62.168

98.69

0.614
  radA Streptococcus pneumoniae Rx1

61.947

98.69

0.611
  radA Streptococcus pneumoniae D39

61.947

98.69

0.611
  radA Streptococcus pneumoniae R6

61.947

98.69

0.611
  radA Streptococcus pneumoniae TIGR4

61.947

98.69

0.611