Detailed information NTG0067499

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: ECO25NV_RS05475
• Genome accession: NZ_AP022326
• Coordinates: 1154253..1155245 (+)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli O25:H4 strain 18044
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 1149253..1160245

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ECO25NV_RS05445 (ECO25NV_10700)	ispD	1149314..1150024 (+)	711	WP_000246149.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
ECO25NV_RS05450 (ECO25NV_10710)	ispF	1150024..1150503 (+)	480	WP_001219237.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
ECO25NV_RS05455 (ECO25NV_10720)	truD	1150500..1151549 (+)	1050	WP_000568920.1	tRNA pseudouridine(13) synthase TruD	-
ECO25NV_RS05460 (ECO25NV_10730)	surE	1151530..1152291 (+)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
ECO25NV_RS05465 (ECO25NV_10740)	pcm	1152285..1152911 (+)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
ECO25NV_RS05470 (ECO25NV_10750)	nlpD	1153096..1154190 (+)	1095	WP_306278776.1	murein hydrolase activator NlpD	-
ECO25NV_RS05475 (ECO25NV_10760)	rpoS	1154253..1155245 (+)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
ECO25NV_RS05480 (ECO25NV_10770)	-	1155416..1155967 (-)	552	WP_001179444.1	flavodoxin family protein	-
ECO25NV_RS05485 (ECO25NV_10780)	-	1156192..1157235 (+)	1044	WP_001161583.1	MBL fold metallo-hydrolase	-
ECO25NV_RS05490 (ECO25NV_10790)	ygbN	1157378..1158742 (-)	1365	WP_000104433.1	GntP family transporter	-
ECO25NV_RS05495 (ECO25NV_10800)	ygbM	1158831..1159607 (-)	777	WP_001136914.1	HPr family phosphocarrier protein	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=67499 ECO25NV_RS05475 WP_000081550.1 1154253..1155245(+) (rpoS) [Escherichia coli O25:H4 strain 18044]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=67499 ECO25NV_RS05475 WP_000081550.1 1154253..1155245(+) (rpoS) [Escherichia coli O25:H4 strain 18044]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGCTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTGATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATCGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCACGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCATTGCTGGACATCCTGGCCGAT
GAAAAAGAGAATGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAATAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715