Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MTX53_RS00975 Genome accession   NZ_CP094971
Coordinates   202446..203813 (+) Length   455 a.a.
NCBI ID   WP_244834328.1    Uniprot ID   -
Organism   Clostridium sp. BJN0001     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 197446..208813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTX53_RS00950 - 197559..198014 (+) 456 WP_244834323.1 CtsR family transcriptional regulator -
  MTX53_RS00955 - 198028..198603 (+) 576 WP_244834324.1 UvrB/UvrC motif-containing protein -
  MTX53_RS00960 - 198587..199603 (+) 1017 WP_244834325.1 ATP--guanido phosphotransferase -
  MTX53_RS00965 - 199629..202067 (+) 2439 WP_244834326.1 ATP-dependent Clp protease ATP-binding subunit -
  MTX53_RS00970 - 202117..202275 (-) 159 WP_244834327.1 hypothetical protein -
  MTX53_RS00975 radA 202446..203813 (+) 1368 WP_244834328.1 DNA repair protein RadA Machinery gene
  MTX53_RS00980 disA 203830..204894 (+) 1065 WP_244834329.1 DNA integrity scanning diadenylate cyclase DisA -
  MTX53_RS00985 - 204954..206072 (+) 1119 WP_244834330.1 PIN/TRAM domain-containing protein -
  MTX53_RS00990 ispD 206087..206803 (+) 717 WP_244834331.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MTX53_RS00995 - 207083..208792 (+) 1710 WP_244834332.1 proline--tRNA ligase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 50457.08 Da        Isoelectric Point: 6.6435

>NTDB_id=674602 MTX53_RS00975 WP_244834328.1 202446..203813(+) (radA) [Clostridium sp. BJN0001]
MAKKSTLFVCQNCGYESIKWLGRCPECKEWNTFNEEAIQDNKQDKKLNYDTISNINKPKSIVDIESGDKQRYDTGIEELN
RVLGGGLVRGSLTLISGEPGIGKSTILLQTADNIASKYGTVLYVSGEESEEQIKIRGDRLKTMSSELFIVSETNLDIVAS
HISNINPKFVIVDSVQTMYKETLTSAPGSVSQVKECVNTIMRICKRNNIPIFLVAHVTKNGELAGPRVLEHMVDTVLYFE
GERSGEYRILRTMKNRFGTTSEIGVFEMKENGLNEVKDPSAYFIEDVECNKDGSAVIGIMEGTRPILIELQALVTETNMA
IPRRTSIGVDNHRLSLILAVLEKKLRIHFFNKDVYVNAVGGMNIDGTAADLGIAVSLISSARNKLFSIDKTIFIGEIGLT
GEIRPVPFSERLIKEAVKMGFENVVISKRNAAKLKCSDINIIGVSDIREAVNKIF

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=674602 MTX53_RS00975 WP_244834328.1 202446..203813(+) (radA) [Clostridium sp. BJN0001]
ATGGCAAAAAAAAGTACGTTATTTGTATGCCAAAATTGTGGATATGAATCAATAAAATGGCTCGGAAGATGCCCTGAATG
CAAAGAGTGGAATACGTTTAACGAAGAAGCAATACAGGATAATAAACAAGATAAAAAACTTAATTATGATACTATTTCAA
ACATTAATAAACCAAAAAGTATCGTAGATATAGAATCTGGAGATAAACAAAGATATGATACTGGGATAGAGGAACTTAAC
AGGGTACTTGGAGGAGGACTTGTCAGGGGGTCTCTTACTCTTATATCGGGAGAACCAGGTATCGGAAAATCAACTATTTT
GCTTCAGACTGCAGATAATATAGCTTCAAAATATGGCACAGTATTATATGTATCTGGAGAGGAATCAGAGGAACAGATAA
AAATTAGAGGCGACAGATTAAAGACGATGAGCAGTGAACTGTTTATTGTATCTGAAACAAACCTTGATATAGTTGCATCT
CATATAAGTAATATAAATCCAAAGTTTGTAATTGTAGATTCTGTTCAGACAATGTATAAAGAAACGCTTACTTCTGCACC
AGGCAGTGTTTCACAGGTAAAAGAATGTGTTAACACGATAATGCGAATATGTAAAAGAAATAATATCCCAATTTTTCTTG
TTGCACACGTTACTAAAAATGGAGAGCTTGCAGGTCCAAGAGTTCTTGAACATATGGTAGATACAGTTCTTTATTTCGAA
GGAGAAAGAAGTGGAGAATATAGAATATTAAGAACTATGAAAAACAGATTTGGAACTACGAGTGAAATAGGTGTTTTTGA
GATGAAGGAAAATGGACTTAATGAAGTAAAAGATCCATCTGCTTATTTTATTGAAGATGTAGAGTGTAATAAGGATGGTT
CAGCGGTTATTGGAATTATGGAAGGAACAAGGCCTATTTTAATAGAACTTCAGGCACTTGTTACTGAAACAAATATGGCT
ATTCCAAGAAGAACTTCAATAGGTGTAGATAATCACAGATTAAGTCTTATTTTAGCTGTACTTGAGAAAAAATTAAGAAT
TCACTTCTTTAACAAGGATGTTTATGTAAATGCTGTAGGTGGAATGAATATAGACGGAACAGCAGCAGACCTTGGTATAG
CGGTTTCTCTTATTTCGAGTGCAAGAAATAAACTTTTTTCTATTGATAAAACTATATTTATAGGTGAAATAGGTCTTACA
GGTGAAATACGTCCTGTACCGTTTTCGGAAAGGCTTATTAAAGAAGCTGTAAAGATGGGTTTTGAAAATGTAGTTATTTC
TAAAAGAAATGCTGCTAAATTGAAATGCTCAGATATAAATATCATAGGAGTATCTGATATAAGAGAAGCTGTAAATAAAA
TTTTTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(345-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(68-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.007

99.56

0.488
  radA Streptococcus pneumoniae Rx1

48.684

100

0.488
  radA Streptococcus pneumoniae D39

48.684

100

0.488
  radA Streptococcus pneumoniae R6

48.684

100

0.488
  radA Streptococcus pneumoniae TIGR4

48.684

100

0.488
  radA Streptococcus mitis SK321

48.684

100

0.488
  radA Streptococcus mitis NCTC 12261

48.684

100

0.488