Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MTR78_RS00435 Genome accession   NZ_CP094933
Coordinates   50692..52071 (+) Length   459 a.a.
NCBI ID   WP_025162202.1    Uniprot ID   A0A1X2JEA2
Organism   Paraclostridium bifermentans strain HD0315_2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 45692..57071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTR78_RS00425 (MTR78_00425) - 46092..48026 (+) 1935 WP_247012420.1 elongation factor G -
  MTR78_RS00430 (MTR78_00430) - 48155..50455 (+) 2301 WP_247012422.1 ATP-dependent Clp protease ATP-binding subunit -
  MTR78_RS00435 (MTR78_00435) radA 50692..52071 (+) 1380 WP_025162202.1 DNA repair protein RadA Machinery gene
  MTR78_RS00440 (MTR78_00440) disA 52073..53143 (+) 1071 WP_021428177.1 DNA integrity scanning diadenylate cyclase DisA -
  MTR78_RS00445 (MTR78_00445) - 53242..54330 (+) 1089 WP_247012424.1 PIN/TRAM domain-containing protein -
  MTR78_RS00450 (MTR78_00450) ispD 54466..55146 (+) 681 WP_247012426.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MTR78_RS00455 (MTR78_00455) ispF 55163..55642 (+) 480 WP_195337002.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50153.14 Da        Isoelectric Point: 8.5410

>NTDB_id=674309 MTR78_RS00435 WP_025162202.1 50692..52071(+) (radA) [Paraclostridium bifermentans strain HD0315_2]
MAKIKTKYVCQECGYENSKWLGKCPECSKWNTFVEEIEEKTSKSNKEVFVIDKSSSRPLNINSIETIKEQRFSTCINELD
RVLGGGVVKGSLVLVGGDPGIGKSTLLIQVSSNVANSGKKVLYISGEESASQIKMRAQRLGIKSDNLYIFAENNLSIIEA
HLESVNPDLIILDSIQTVFSPEITSAPGTVSQIKEGTSRFMKISKKMGISTFVVGHVTKEGALAGPKVLEHMVDTVLYFE
GERFNTYRLIRAVKNRFGSTNELGVFEMREVGLVELENPSKILIAEKPKDVAGSIIISTVEGTRPMLLELQALASPTSFG
IPKRTATGVDYNRVSLLMAVLEKRVGMQIQNQDIYINVVGGIKINEPSIDLGIVMAIASSFRNIPIDGNVAITGEVGLTG
EVRAVSFIEKRIAECKKLGFTKIVIPKSNYEAVKDVKGIDICPVDSVRQAINMVLGGNR

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=674309 MTR78_RS00435 WP_025162202.1 50692..52071(+) (radA) [Paraclostridium bifermentans strain HD0315_2]
ATGGCTAAAATAAAAACTAAATATGTGTGTCAAGAATGTGGGTATGAGAATAGCAAATGGCTAGGAAAATGTCCAGAATG
TAGCAAATGGAATACATTTGTCGAAGAAATAGAAGAAAAAACTTCAAAATCAAATAAAGAAGTTTTTGTTATAGATAAAT
CATCATCTAGACCTTTAAACATAAACTCGATAGAAACTATAAAAGAGCAAAGATTTTCAACTTGCATAAACGAGTTAGAT
AGAGTTTTAGGTGGAGGAGTAGTTAAAGGATCATTAGTTTTAGTCGGAGGGGATCCAGGTATTGGTAAGTCAACATTGCT
TATACAAGTTTCAAGTAATGTTGCTAATAGCGGTAAAAAAGTTTTATACATATCAGGAGAAGAATCTGCATCTCAAATAA
AAATGAGGGCTCAAAGATTAGGAATAAAGTCCGATAATTTATATATATTTGCTGAAAATAACTTAAGTATAATAGAAGCT
CACTTAGAAAGTGTAAATCCAGACTTAATTATATTAGACTCTATACAAACTGTTTTTAGTCCTGAAATTACATCTGCTCC
AGGTACTGTAAGCCAGATAAAGGAAGGTACTTCGAGATTTATGAAAATTTCTAAAAAAATGGGTATATCAACCTTTGTAG
TTGGACATGTAACTAAAGAAGGTGCACTAGCAGGACCTAAGGTATTAGAACATATGGTAGACACTGTATTATATTTTGAA
GGAGAACGATTCAATACGTATAGATTGATAAGAGCTGTAAAAAATAGATTTGGGTCTACAAATGAATTAGGTGTATTTGA
GATGAGAGAAGTAGGATTAGTTGAGTTAGAAAATCCATCTAAAATACTTATAGCCGAAAAACCAAAAGATGTAGCAGGGT
CTATAATTATATCTACAGTAGAAGGTACTAGACCAATGCTTTTAGAATTGCAAGCACTAGCATCACCTACTAGCTTTGGT
ATTCCTAAAAGAACGGCTACAGGAGTTGATTATAATAGAGTATCATTACTTATGGCTGTTCTTGAAAAACGTGTTGGTAT
GCAAATACAAAACCAGGATATATATATAAATGTTGTGGGAGGAATAAAAATTAATGAGCCGTCTATAGATCTAGGCATAG
TTATGGCTATAGCTTCTAGTTTTAGAAATATACCCATAGATGGGAATGTAGCAATTACAGGAGAAGTTGGATTAACAGGA
GAAGTAAGAGCGGTGAGCTTTATAGAAAAGAGAATCGCAGAATGTAAGAAGCTAGGTTTCACTAAAATCGTTATTCCAAA
GAGCAATTATGAGGCTGTTAAAGATGTAAAAGGGATAGATATATGTCCAGTGGATAGTGTAAGACAAGCTATAAATATGG
TATTAGGAGGAAATAGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(68-221)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(349-452)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1X2JEA2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

57.205

99.782

0.571
  radA Streptococcus pneumoniae Rx1

52.193

99.346

0.519
  radA Streptococcus pneumoniae D39

52.193

99.346

0.519
  radA Streptococcus pneumoniae R6

52.193

99.346

0.519
  radA Streptococcus pneumoniae TIGR4

52.193

99.346

0.519
  radA Streptococcus mitis NCTC 12261

51.974

99.346

0.516
  radA Streptococcus mitis SK321

51.974

99.346

0.516