Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   SVA_RS03045 Genome accession   NZ_AP014936
Coordinates   617133..618608 (-) Length   491 a.a.
NCBI ID   WP_096458636.1    Uniprot ID   A0A1B4V3V6
Organism   Sulfurifustis variabilis strain skN76     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 612133..623608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SVA_RS03015 (SVA_0617) - 613622..613891 (-) 270 WP_096458618.1 HPr family phosphocarrier protein -
  SVA_RS03020 (SVA_0618) - 613888..614313 (-) 426 WP_096458621.1 PTS sugar transporter subunit IIA -
  SVA_RS03025 (SVA_0619) rapZ 614450..615304 (-) 855 WP_096458624.1 RNase adapter RapZ -
  SVA_RS03030 (SVA_0620) hprK 615301..616260 (-) 960 WP_096458627.1 HPr(Ser) kinase/phosphatase -
  SVA_RS03035 (SVA_0621) - 616235..616720 (-) 486 WP_096458630.1 PTS sugar transporter subunit IIA -
  SVA_RS03040 (SVA_0622) hpf 616789..617112 (-) 324 WP_096458633.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  SVA_RS03045 (SVA_0623) rpoN/rpoN1 617133..618608 (-) 1476 WP_096458636.1 RNA polymerase factor sigma-54 Machinery gene
  SVA_RS03050 (SVA_0624) lptB 618727..619452 (-) 726 WP_096458639.1 LPS export ABC transporter ATP-binding protein -
  SVA_RS03055 (SVA_0625) lptA 619449..620054 (-) 606 WP_096458642.1 lipopolysaccharide transport periplasmic protein LptA -
  SVA_RS03060 (SVA_0626) lptC 620071..620505 (-) 435 WP_169923944.1 LPS export ABC transporter periplasmic protein LptC -
  SVA_RS03065 (SVA_0627) - 620502..621098 (-) 597 WP_096458648.1 KdsC family phosphatase -
  SVA_RS03070 (SVA_0628) - 621201..622202 (-) 1002 WP_096458651.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 491 a.a.        Molecular weight: 55371.43 Da        Isoelectric Point: 5.0082

>NTDB_id=67419 SVA_RS03045 WP_096458636.1 617133..618608(-) (rpoN/rpoN1) [Sulfurifustis variabilis strain skN76]
MKQSLDLRLGQHLTITPQLQQAIRLLQLSTVELQQEIQEALETNPLLEEGEESENRDANGEGEAGESETEYEAEASEPAD
LDMEQADLGETSAESDWDEAFDLPLASGSRNEGDERPDIDARNSRPQTLREHLLWQMHMTSFSVTDRRVAEALIDAINED
GYLTCKLEEVQQILAKESPPTQAELDEIEAVLHQVQNFDPIGVGARDLAECLLLQLKALDPETPYLREALRLATPEHLAL
LGGRDYNQLRRNLKVTPETLHHAVQLIQRLNPRPGGTVQSVAANYIVPDIIVRKIRGIWRADLNQEVAPRLRINRMYERL
IHRGDGSRDNRYLQDQLQQARWFIKSLNSRNETLLKVARTIVDRQRAFFDHGPEAMKPLVLHDIAEAVSMHESTISRVTT
NKYMLTPRGIYELKYFFSSHVGTADGGACSATAIRSMIKKLVENEPPTKPISDSKIAEILAGQGIHVARRTVAKYRESIS
IPPSNQRKSII

Nucleotide


Download         Length: 1476 bp        

>NTDB_id=67419 SVA_RS03045 WP_096458636.1 617133..618608(-) (rpoN/rpoN1) [Sulfurifustis variabilis strain skN76]
ATGAAGCAGTCACTCGACCTTCGGCTAGGTCAGCATCTCACGATTACCCCCCAGTTGCAGCAGGCCATCCGCCTGCTGCA
ACTGTCCACCGTGGAGCTTCAGCAGGAGATCCAGGAAGCGCTCGAAACGAATCCGCTGCTCGAGGAAGGCGAGGAATCGG
AGAACCGCGACGCCAACGGCGAGGGCGAGGCGGGGGAGTCGGAGACCGAGTACGAGGCCGAGGCGTCCGAGCCGGCGGAC
CTCGACATGGAACAGGCGGACCTCGGCGAAACCTCGGCGGAAAGCGACTGGGACGAGGCCTTCGACCTCCCCCTCGCTTC
GGGCAGCCGGAACGAGGGCGACGAGCGACCCGATATCGACGCCCGCAACAGCCGTCCGCAAACGCTCCGCGAGCATCTCC
TCTGGCAGATGCACATGACGTCCTTCTCGGTGACGGACCGGCGGGTCGCCGAGGCCCTGATCGACGCGATCAACGAGGAC
GGCTACCTCACCTGCAAGCTCGAGGAGGTCCAGCAGATCCTCGCGAAAGAGTCGCCGCCGACCCAGGCGGAGCTGGACGA
AATCGAGGCCGTGCTGCACCAGGTCCAGAACTTCGACCCGATCGGCGTCGGCGCCCGCGACCTGGCGGAATGCCTGCTGC
TCCAGCTCAAGGCGCTCGATCCCGAAACGCCCTACCTCCGTGAGGCCCTGCGGCTCGCGACGCCCGAGCACCTGGCGCTC
CTCGGCGGGCGGGACTACAACCAGCTGCGGCGCAATCTGAAGGTGACGCCCGAAACCCTGCACCACGCCGTGCAGCTCAT
CCAGCGGCTCAACCCGCGGCCGGGCGGGACGGTCCAGTCGGTCGCGGCCAACTACATCGTCCCGGATATCATCGTGCGCA
AGATCAGGGGCATCTGGCGCGCCGACCTGAACCAGGAAGTCGCGCCGCGGCTGCGCATCAACCGGATGTACGAGCGCCTG
ATCCACCGCGGCGACGGCAGCCGCGACAACCGCTATCTGCAGGACCAGCTCCAGCAGGCGCGCTGGTTCATCAAGAGCCT
GAACAGCCGCAACGAGACCCTGCTCAAGGTTGCCCGCACCATCGTCGACCGCCAGCGCGCCTTTTTCGACCACGGTCCGG
AGGCGATGAAGCCGCTCGTGCTGCACGACATCGCCGAGGCGGTCTCGATGCACGAGTCGACGATCTCGCGCGTGACCACC
AACAAGTACATGCTCACGCCGCGCGGAATCTACGAGCTCAAGTACTTTTTCTCGAGCCACGTGGGAACCGCCGACGGGGG
CGCGTGCTCCGCGACGGCCATCCGCTCCATGATCAAGAAGCTCGTGGAAAACGAACCGCCGACGAAGCCGATCAGCGACA
GCAAGATCGCCGAGATCCTCGCCGGGCAGGGCATTCATGTGGCGCGCCGCACCGTCGCCAAGTACCGGGAATCGATCAGC
ATCCCGCCGTCCAACCAGCGCAAGTCCATCATCTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(120-317)

Sigma-54, DNA binding domain (Sigma54_DBD)

(331-488)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4V3V6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

47.929

100

0.495

Multiple sequence alignment