Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MUA60_RS14425 Genome accession   NZ_CP094824
Coordinates   2733444..2734814 (-) Length   456 a.a.
NCBI ID   WP_262648868.1    Uniprot ID   -
Organism   Mammaliicoccus sciuri strain IVB6216     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2728444..2739814
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUA60_RS14400 (MUA60_14360) cysE 2728836..2729477 (-) 642 WP_262648862.1 serine O-acetyltransferase -
  MUA60_RS14405 (MUA60_14365) gltX 2729733..2731187 (-) 1455 WP_262648864.1 glutamate--tRNA ligase -
  MUA60_RS14410 (MUA60_14370) ispF 2731203..2731682 (-) 480 WP_262648866.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  MUA60_RS14415 (MUA60_14375) ispD 2731675..2732361 (-) 687 WP_262648867.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MUA60_RS14420 (MUA60_14380) - 2732363..2733421 (-) 1059 WP_037589912.1 PIN/TRAM domain-containing protein -
  MUA60_RS14425 (MUA60_14385) radA 2733444..2734814 (-) 1371 WP_262648868.1 DNA repair protein RadA Machinery gene
  MUA60_RS14430 (MUA60_14390) - 2734970..2737423 (-) 2454 WP_262648869.1 ATP-dependent Clp protease ATP-binding subunit -
  MUA60_RS14435 (MUA60_14395) - 2737440..2738447 (-) 1008 WP_262648871.1 protein arginine kinase -
  MUA60_RS14440 (MUA60_14400) - 2738448..2738984 (-) 537 WP_262648872.1 excinuclease ABC subunit B -
  MUA60_RS14445 (MUA60_14405) - 2738999..2739460 (-) 462 WP_025904841.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50491.27 Da        Isoelectric Point: 7.7103

>NTDB_id=673457 MUA60_RS14425 WP_262648868.1 2733444..2734814(-) (radA) [Mammaliicoccus sciuri strain IVB6216]
MAKKKVIFECLACGYQSPKWMGKCPNCGAWNQMEESIEHKETKGPKNTFKSDVPAQKIEKLKDVKEEDTPRIKTKINEFN
RVLGGGVVEGSLVLIGGDPGIGKSTLLLQICAALSQQKNILYVSGEESVRQTKLRAERLVEDSGELNVYAETNLEIIHSM
VQKEKPDVLVIDSIQTVFHPEVTSAPGSVSQVRECTQNLMHIAKQMNIATFIVGHVTKDGQIAGPRLLEHMVDTVLYFEG
DTHHAYRILRAVKNRFGSTNEMGIFEMKQTGLNEVKNPSEMFLEERTKHVSGSTIVATMEGTRPILVEVQALVTPTTFHN
PRRMATGIDHNRLSLLMAVLEKKENFLLQQQDAYIKIAGGVKLSEPAVDLSIVIAIASSFQDKPTKGDDCFVGEVGLTGE
VRRVARIDQRVQEAAKLGFKRIIIPKNNIKGLEFPEGTEVIGVSTTREALKIAFNA

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=673457 MUA60_RS14425 WP_262648868.1 2733444..2734814(-) (radA) [Mammaliicoccus sciuri strain IVB6216]
ATGGCCAAAAAGAAAGTAATATTTGAGTGTTTAGCCTGCGGATATCAATCTCCAAAATGGATGGGGAAATGCCCTAATTG
TGGTGCATGGAACCAAATGGAAGAATCAATAGAACATAAAGAAACTAAAGGTCCTAAAAATACATTCAAGTCAGATGTAC
CCGCACAAAAAATAGAAAAATTAAAAGACGTTAAAGAAGAAGATACACCTAGAATTAAGACTAAAATCAATGAATTTAAT
CGCGTATTAGGTGGCGGTGTTGTAGAAGGTTCGCTTGTACTCATTGGTGGGGACCCTGGTATTGGTAAATCCACATTATT
ATTACAAATTTGTGCGGCTTTATCTCAACAAAAAAATATCTTATATGTTTCAGGTGAAGAATCTGTAAGACAAACAAAAT
TAAGAGCTGAAAGATTAGTTGAAGATTCAGGAGAATTAAATGTTTATGCTGAAACGAATTTAGAGATTATACATAGTATG
GTTCAAAAAGAAAAACCAGATGTATTAGTAATAGATTCCATTCAAACTGTCTTCCACCCTGAAGTCACTTCTGCGCCAGG
TTCTGTTTCTCAAGTTAGAGAATGTACACAAAATTTAATGCATATCGCAAAACAAATGAATATTGCTACATTTATTGTTG
GACACGTAACGAAAGATGGACAAATTGCTGGTCCACGTTTGTTAGAACATATGGTAGATACAGTATTATATTTTGAAGGC
GATACACATCATGCATACAGAATATTAAGAGCAGTCAAAAACCGTTTTGGTTCGACAAATGAAATGGGTATTTTTGAAAT
GAAGCAAACGGGTCTTAACGAAGTTAAAAATCCGTCTGAAATGTTCTTAGAAGAAAGAACGAAACATGTATCGGGTTCGA
CTATTGTGGCAACTATGGAAGGTACGAGACCTATTTTAGTTGAAGTACAAGCTTTAGTAACGCCAACGACTTTTCATAAT
CCACGAAGAATGGCAACAGGTATCGATCACAATAGATTAAGCTTATTGATGGCTGTATTAGAGAAGAAAGAGAATTTCTT
ATTACAACAACAAGACGCATATATCAAAATTGCCGGTGGTGTAAAATTAAGTGAACCCGCAGTAGATTTAAGTATTGTTA
TCGCGATAGCTTCAAGTTTCCAAGATAAACCTACTAAAGGTGATGATTGTTTCGTAGGAGAGGTTGGCTTAACAGGTGAA
GTAAGAAGGGTTGCTAGAATTGATCAACGTGTACAAGAAGCGGCTAAATTAGGATTTAAACGTATTATCATACCTAAAAA
TAATATTAAAGGTTTAGAATTCCCTGAAGGTACAGAAGTTATTGGCGTATCAACAACAAGAGAAGCATTGAAAATTGCTT
TTAATGCATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(349-428)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.502

100

0.658
  radA Streptococcus pneumoniae Rx1

61.674

99.561

0.614
  radA Streptococcus pneumoniae D39

61.674

99.561

0.614
  radA Streptococcus pneumoniae R6

61.674

99.561

0.614
  radA Streptococcus pneumoniae TIGR4

61.674

99.561

0.614
  radA Streptococcus mitis NCTC 12261

61.674

99.561

0.614
  radA Streptococcus mitis SK321

61.674

99.561

0.614