Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   SCL_RS10890 Genome accession   NZ_AP014879
Coordinates   2272727..2274226 (+) Length   499 a.a.
NCBI ID   WP_096361237.1    Uniprot ID   A0A1B4XI70
Organism   Sulfuricaulis limicola strain HA5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2267727..2279226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SCL_RS10865 (SCL_2209) - 2269112..2270086 (+) 975 WP_096361233.1 KpsF/GutQ family sugar-phosphate isomerase -
  SCL_RS10870 (SCL_2210) kdsC 2270196..2270777 (+) 582 WP_096361234.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  SCL_RS10875 (SCL_2211) lptC 2270774..2271208 (+) 435 WP_096361235.1 LPS export ABC transporter periplasmic protein LptC -
  SCL_RS10880 (SCL_2212) lptA 2271214..2271750 (+) 537 WP_096361236.1 lipopolysaccharide transport periplasmic protein LptA -
  SCL_RS10885 (SCL_2213) lptB 2271832..2272557 (+) 726 WP_096361952.1 LPS export ABC transporter ATP-binding protein -
  SCL_RS10890 (SCL_2214) rpoN/rpoN1 2272727..2274226 (+) 1500 WP_096361237.1 RNA polymerase factor sigma-54 Machinery gene
  SCL_RS10895 (SCL_2215) hprK 2274448..2275530 (+) 1083 WP_197702612.1 HPr(Ser) kinase/phosphatase -
  SCL_RS10900 (SCL_2216) rapZ 2275531..2276385 (+) 855 WP_096361238.1 RNase adapter RapZ -
  SCL_RS10905 (SCL_2217) - 2276391..2276801 (+) 411 WP_096361239.1 PTS sugar transporter subunit IIA -
  SCL_RS10910 (SCL_2218) - 2276801..2277070 (+) 270 WP_096361240.1 HPr family phosphocarrier protein -
  SCL_RS10915 (SCL_2219) ptsP 2277115..2278842 (+) 1728 WP_096361241.1 phosphoenolpyruvate--protein phosphotransferase -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55678.48 Da        Isoelectric Point: 4.5694

>NTDB_id=67222 SCL_RS10890 WP_096361237.1 2272727..2274226(+) (rpoN/rpoN1) [Sulfuricaulis limicola strain HA5]
MKQSLDLKLGQHLTITPQLQQAIRLLQLSSIELQQEIQEALESNPLLEENEREDETPEAKNETELPASDTADANGEDSGE
SSYDEAEALPATDSDTGPDADSDADWDETFEPMTAPTASRSASDDDYPDIDARNSPPQTLRDHLLWQMQMTPFSDTDKQI
ALALIDAVNEDGYLSCKLEEIQQALGKNHAIEMDEIEAVLHQIQNFDPLGVGARDLAECLRLQMKVLPPDLPDLKAATEL
ATPVNLALLASRDYTQLRRNLKLTAEELQQAISLIQGLNPRPGNSVTSSQAGYVVPDIVVRKFRGVWRAELNPDVSPRLR
INRQYEKMIHRGDSSADNRYLQDQLQQARWFIKSLASRNDTLLKVARTIVDRQRAFFEHGAEAMKPLVLHDVAETVSMHE
STISRVTTNKYMLTPRGIFELKYFFSSHVTTADGGTCSATAIRSIIKKLVECETSTKPISDSKIAEILAEQGINVARRTV
AKYRESMSIPPSNQRKSLV

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=67222 SCL_RS10890 WP_096361237.1 2272727..2274226(+) (rpoN/rpoN1) [Sulfuricaulis limicola strain HA5]
ATGAAGCAGTCACTTGACCTTAAACTGGGTCAACATTTAACCATCACCCCCCAACTGCAGCAGGCCATCCGGCTGCTGCA
GCTTTCTTCCATCGAACTGCAACAGGAAATCCAGGAAGCGCTCGAAAGCAACCCGTTGCTCGAGGAGAACGAGCGCGAAG
ACGAAACGCCGGAAGCCAAGAACGAGACCGAGCTTCCCGCCTCCGATACCGCCGACGCCAATGGCGAGGACAGCGGCGAG
TCCTCCTACGATGAGGCCGAGGCGCTGCCCGCCACCGACTCCGACACCGGCCCCGATGCGGACAGCGACGCCGACTGGGA
CGAGACTTTCGAACCCATGACGGCGCCCACCGCCAGCCGCTCCGCCTCCGACGACGACTACCCCGACATCGACGCGCGCA
ACAGTCCGCCGCAGACGCTGCGTGACCATCTGTTGTGGCAGATGCAGATGACGCCGTTCTCGGACACCGACAAGCAGATC
GCACTGGCCCTGATCGACGCCGTGAACGAGGACGGCTACCTCAGCTGCAAGCTCGAGGAAATCCAGCAGGCGCTGGGAAA
AAATCATGCCATCGAGATGGATGAAATCGAGGCGGTATTGCACCAGATCCAGAACTTCGACCCGCTGGGCGTCGGCGCGC
GCGACCTGGCCGAGTGCCTGCGCCTGCAGATGAAAGTGCTGCCGCCCGACCTGCCCGACCTGAAGGCGGCGACCGAACTT
GCCACGCCGGTCAACCTCGCCCTGCTGGCAAGCCGGGATTACACCCAGCTGCGGCGCAATCTCAAGCTCACGGCGGAGGA
ATTGCAACAGGCGATAAGCCTCATCCAGGGCCTCAATCCGCGACCGGGCAATTCCGTCACCTCGTCACAGGCCGGTTACG
TGGTGCCCGACATCGTCGTGCGGAAATTCCGCGGCGTGTGGCGCGCGGAACTGAATCCGGACGTGTCGCCGCGTCTGCGC
ATCAACCGCCAGTACGAAAAAATGATTCACCGCGGCGATTCCAGCGCCGACAACCGCTACCTGCAGGACCAGCTGCAACA
GGCGCGCTGGTTCATCAAGAGCCTCGCTTCCCGCAACGACACGCTGCTCAAGGTCGCGCGCACCATCGTGGACCGTCAGC
GCGCCTTCTTCGAGCACGGCGCGGAGGCGATGAAACCGCTGGTGCTGCACGACGTCGCCGAGACCGTGAGCATGCACGAG
TCCACCATCTCGCGCGTCACCACCAACAAGTACATGCTGACACCGCGCGGGATCTTCGAACTCAAGTATTTCTTTTCCAG
CCATGTCACCACCGCGGATGGCGGCACCTGTTCGGCGACCGCGATCCGCTCGATCATCAAGAAACTGGTGGAGTGCGAAA
CGTCGACCAAGCCCATCAGCGACAGCAAGATCGCCGAGATCCTGGCCGAGCAGGGCATCAACGTCGCCCGCCGCACCGTG
GCCAAATACCGCGAGTCCATGAGCATCCCGCCTTCCAACCAGCGCAAGTCGCTGGTGTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(132-325)

Sigma-54, DNA binding domain (Sigma54_DBD)

(339-496)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XI70

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

48.462

100

0.505

Multiple sequence alignment