Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   KNN_RS29850 Genome accession   NZ_AP014864
Coordinates   5797751..5799127 (-) Length   458 a.a.
NCBI ID   WP_001085202.1    Uniprot ID   -
Organism   Bacillus thuringiensis serovar tolworthi strain Pasteur Institute Standard strain     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5792751..5804127
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KNN_RS29830 (KNN_06162) ispF 5794113..5794589 (-) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  KNN_RS29835 (KNN_06163) ispD 5794706..5795386 (-) 681 WP_000288283.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  KNN_RS29840 (KNN_06164) - 5795403..5796512 (-) 1110 WP_000919677.1 PIN/TRAM domain-containing protein -
  KNN_RS29845 (KNN_06165) disA 5796674..5797747 (-) 1074 WP_000392158.1 DNA integrity scanning diadenylate cyclase DisA -
  KNN_RS29850 (KNN_06166) radA 5797751..5799127 (-) 1377 WP_001085202.1 DNA repair protein RadA Machinery gene
  KNN_RS29855 (KNN_06167) clpC 5799223..5801658 (-) 2436 WP_000971184.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  KNN_RS29860 (KNN_06168) - 5801681..5802745 (-) 1065 WP_060852504.1 protein arginine kinase -
  KNN_RS29865 (KNN_06169) - 5802750..5803298 (-) 549 WP_000128399.1 UvrB/UvrC motif-containing protein -
  KNN_RS29870 (KNN_06171) ctsR 5803469..5803930 (-) 462 WP_001244558.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49844.48 Da        Isoelectric Point: 7.1422

>NTDB_id=67204 KNN_RS29850 WP_001085202.1 5797751..5799127(-) (radA) [Bacillus thuringiensis serovar tolworthi strain Pasteur Institute Standard strain]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNSLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADASYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPEGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=67204 KNN_RS29850 WP_001085202.1 5797751..5799127(-) (radA) [Bacillus thuringiensis serovar tolworthi strain Pasteur Institute Standard strain]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGTTATCAATCACCAAAATATATGGGTAAATGTCCAGGGTG
TGGTCAATGGAATTCGCTTGTTGAAGAGATGGAACCCGTTGTATCATCCAGACGCCTAAATTATGCAAATGCAATTCAAA
CAGAAGTAACAAAACCAAGACGTCTTACAGAAGTAGAAACAAAGTCTGAGGCACGTATTGAAACAAAATTTCAGGAGTTT
AACCGTGTACTAGGTGGCGGAATTGTAGATGGATCTTTAGTACTTATTGGTGGGGACCCTGGGATTGGAAAATCAACGTT
ATTATTACAGATTTCCTCGCAATTAGCAGATGCTTCATATGATGTATTGTATATATCAGGTGAGGAGTCAGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTACATGTAAATGGTAGTAATCTATTTGTTGTATCAGAGACAGATTTACAGCGTATTGCA
GCACATATTGAAGAGATGAATCCTGCTTTTGTTGTTATTGATTCTATTCAAACGATACATTTACCTGAAGTGACATCAGC
TCCTGGTAGTGTGGCGCAAGTACGTGAATGTACAGCAGAATTAATGAAACTTGCAAAAACAAAGGGAATCCCAATTTTTA
TCGTAGGGCATGTGACAAAAGAAGGTGCAATTGCGGGACCGCGTATGCTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGATCGACACCATACATATCGTATTTTACGAGCTGTAAAGAATCGTTTTGGTTCTACGAATGAAATGGGTATCTT
TGAAATGAAAGAACTTGGCCTTGCAGAAGTCTTAAATCCTTCTGAAATTTTCCTTGAGGAAAGACCAGTTGGAGTTGCAG
GTTCAACAGTAGTTGCCTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAGGCATTAATTTCCCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCGACAGGAATTGATCATAATCGTGTCTCGCTTATTATGGCGGTACTAGAGAAAAGAACAGG
TTTACTATTACAAAATCAAGATGCGTATTTAAAAGTAGCTGGTGGTTTGAAATTAGATGAACCGGCAATTGATTTAGCTG
TTGCCTTAAGTATAGCTTCAAGTTTTAGAGATAAATCTACGGCACCAACTGATGCGGTAATAGGAGAAGTTGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATCGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGTGCTATCATTCC
TAGAAAAAATTTAGGGGGGTGGACAATTCCAGAGGGGATTGAGGTTGTAGGTGTTTCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

AAA domain (AAA_25)

(60-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.728

100

0.749
  radA Streptococcus pneumoniae Rx1

64.018

98.908

0.633
  radA Streptococcus pneumoniae D39

64.018

98.908

0.633
  radA Streptococcus pneumoniae R6

64.018

98.908

0.633
  radA Streptococcus pneumoniae TIGR4

64.018

98.908

0.633
  radA Streptococcus mitis SK321

64.018

98.908

0.633
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631

Multiple sequence alignment