Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   H7R25_RS09970 Genome accession   NZ_AP022261
Coordinates   2095146..2096156 (+) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli strain WP8-S18-ESBL-07     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2090146..2101156
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R25_RS09940 (WP8S18E07_19380) nudB 2091870..2092322 (+) 453 WP_076611058.1 dihydroneopterin triphosphate diphosphatase -
  H7R25_RS09945 (WP8S18E07_19390) yebC 2092351..2093091 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  H7R25_RS09950 (WP8S18E07_19400) ruvC 2093126..2093647 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  H7R25_RS09955 (WP8S18E07_19410) yebB 2093649..2094251 (-) 603 WP_001024936.1 YebB family permuted papain-like enzyme -
  H7R25_RS09960 yobI 2094322..2094387 (+) 66 WP_072123646.1 stress response small protein YobI -
  H7R25_RS09965 (WP8S18E07_19420) ruvA 2094526..2095137 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  H7R25_RS09970 (WP8S18E07_19430) ruvB 2095146..2096156 (+) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  H7R25_RS09975 (WP8S18E07_19440) znuB 2096308..2097093 (-) 786 WP_000571478.1 zinc ABC transporter permease subunit ZnuB -
  H7R25_RS09980 (WP8S18E07_19450) znuC 2097090..2097845 (-) 756 WP_000202988.1 zinc ABC transporter ATP-binding protein ZnuC -
  H7R25_RS09985 (WP8S18E07_19460) znuA 2097924..2098856 (+) 933 WP_001376899.1 zinc ABC transporter substrate-binding protein ZnuA -
  H7R25_RS09990 (WP8S18E07_19470) mepM 2098872..2100194 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=67191 H7R25_RS09970 WP_000568522.1 2095146..2096156(+) (ruvB) [Escherichia coli strain WP8-S18-ESBL-07]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=67191 H7R25_RS09970 WP_000568522.1 2095146..2096156(+) (ruvB) [Escherichia coli strain WP8-S18-ESBL-07]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCCGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCTGATATCGCTGCTCAGGCGCTGGATATGCTGAATGTCGATGCCGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGCCTGGATAACCTGGCGGCTGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494

Multiple sequence alignment