Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MUA64_RS08255 Genome accession   NZ_CP094745
Coordinates   1671604..1672578 (-) Length   324 a.a.
NCBI ID   WP_061824406.1    Uniprot ID   -
Organism   Staphylococcus aureus strain IVB6250     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1666604..1677578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUA64_RS08215 (MUA64_08215) gcvT 1667105..1668196 (-) 1092 WP_000093347.1 glycine cleavage system aminomethyltransferase GcvT -
  MUA64_RS08220 (MUA64_08220) - 1668355..1668879 (-) 525 WP_001015125.1 shikimate kinase -
  MUA64_RS08225 (MUA64_08225) - 1668869..1669015 (-) 147 WP_001789879.1 hypothetical protein -
  MUA64_RS08230 (MUA64_08230) comGF 1669112..1669609 (-) 498 WP_001794328.1 competence type IV pilus minor pilin ComGF Machinery gene
  MUA64_RS08235 (MUA64_08235) comGE 1669527..1669826 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  MUA64_RS08240 (MUA64_08240) comGD 1669813..1670259 (-) 447 WP_001791207.1 competence type IV pilus minor pilin ComGD Machinery gene
  MUA64_RS08245 (MUA64_08245) comGC 1670237..1670548 (-) 312 WP_000472257.1 competence type IV pilus major pilin ComGC Machinery gene
  MUA64_RS08250 (MUA64_08250) comGB 1670562..1671632 (-) 1071 WP_000776412.1 competence type IV pilus assembly protein ComGB Machinery gene
  MUA64_RS08255 (MUA64_08255) comGA 1671604..1672578 (-) 975 WP_061824406.1 competence type IV pilus ATPase ComGA Machinery gene
  MUA64_RS08260 (MUA64_08260) - 1672631..1673254 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  MUA64_RS08265 (MUA64_08265) - 1673251..1673580 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  MUA64_RS08270 (MUA64_08270) - 1673580..1674566 (-) 987 WP_000161314.1 ROK family glucokinase -
  MUA64_RS08275 (MUA64_08275) - 1674563..1674766 (-) 204 WP_000087561.1 YqgQ family protein -
  MUA64_RS08280 (MUA64_08280) - 1674747..1676210 (-) 1464 WP_045179195.1 rhomboid family intramembrane serine protease -
  MUA64_RS08285 (MUA64_08285) - 1676222..1676761 (-) 540 WP_000164553.1 5-formyltetrahydrofolate cyclo-ligase -
  MUA64_RS08290 (MUA64_08290) rpmG 1676970..1677119 (-) 150 WP_153081322.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36926.01 Da        Isoelectric Point: 9.0398

>NTDB_id=671838 MUA64_RS08255 WP_061824406.1 1671604..1672578(-) (comGA) [Staphylococcus aureus strain IVB6250]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKRGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=671838 MUA64_RS08255 WP_061824406.1 1671604..1672578(-) (comGA) [Staphylococcus aureus strain IVB6250]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACGAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTGCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.691

100

0.997
  comGA Staphylococcus aureus N315

99.691

100

0.997