Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KNN_RS08530 Genome accession   NZ_AP014864
Coordinates   1593219..1593653 (+) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus thuringiensis serovar tolworthi strain Pasteur Institute Standard strain     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1588219..1598653
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KNN_RS08510 (KNN_01762) - 1589133..1590296 (-) 1164 WP_000434582.1 aminotransferase A -
  KNN_RS08515 (KNN_01763) - 1590419..1590619 (-) 201 WP_000929277.1 hypothetical protein -
  KNN_RS08520 (KNN_01764) kinB 1590930..1592204 (+) 1275 WP_000420370.1 sporulation sensor histidine kinase KinB -
  KNN_RS08525 (KNN_01765) - 1592243..1592992 (-) 750 WP_000388471.1 DUF3967 domain-containing protein -
  KNN_RS08530 (KNN_01766) nucA/comI 1593219..1593653 (+) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  KNN_RS08535 (KNN_01767) comJ 1593690..1594079 (+) 390 WP_000424030.1 competence protein ComJ -
  KNN_RS08540 (KNN_01768) metE 1594118..1596406 (-) 2289 WP_001007607.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  KNN_RS08545 (KNN_01769) - 1596920..1597477 (-) 558 WP_000062061.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=67178 KNN_RS08530 WP_000811494.1 1593219..1593653(+) (nucA/comI) [Bacillus thuringiensis serovar tolworthi strain Pasteur Institute Standard strain]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=67178 KNN_RS08530 WP_000811494.1 1593219..1593653(+) (nucA/comI) [Bacillus thuringiensis serovar tolworthi strain Pasteur Institute Standard strain]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGTTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment