Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   MUA81_RS00170 Genome accession   NZ_CP094697
Coordinates   36152..37501 (-) Length   449 a.a.
NCBI ID   WP_262900862.1    Uniprot ID   -
Organism   Staphylococcus simulans strain IVB6244     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 25366..89431 36152..37501 within 0
IScluster/Tn 30004..35486 36152..37501 flank 666


Gene organization within MGE regions


Location: 25366..89431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUA81_RS00110 (MUA81_00110) - 25366..26700 (+) 1335 WP_262900854.1 YycH family regulatory protein -
  MUA81_RS00115 (MUA81_00115) - 26697..27485 (+) 789 WP_262900857.1 two-component system regulatory protein YycI -
  MUA81_RS00120 (MUA81_00120) - 27781..28581 (+) 801 WP_031262349.1 MBL fold metallo-hydrolase -
  MUA81_RS00125 (MUA81_00125) rlmH 29040..29519 (+) 480 WP_107547207.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -
  MUA81_RS00130 (MUA81_00130) - 29593..29979 (+) 387 WP_262559937.1 hypothetical protein -
  MUA81_RS00135 (MUA81_00135) - 30004..30678 (+) 675 WP_262604229.1 IS6 family transposase -
  MUA81_RS00140 (MUA81_00140) - 30720..31634 (+) 915 WP_262900858.1 protein rep -
  MUA81_RS00145 (MUA81_00145) - 31780..31980 (-) 201 WP_262611019.1 hypothetical protein -
  MUA81_RS00150 (MUA81_00150) - 32770..33066 (-) 297 WP_096608588.1 hypothetical protein -
  MUA81_RS00155 (MUA81_00155) - 33760..34674 (-) 915 WP_316960054.1 ISL3 family transposase -
  MUA81_RS00160 (MUA81_00160) - 34812..35486 (+) 675 WP_262604229.1 IS6 family transposase -
  MUA81_RS00165 (MUA81_00165) - 35531..36130 (-) 600 Protein_30 recombinase family protein -
  MUA81_RS00170 (MUA81_00170) ccrA 36152..37501 (-) 1350 WP_262900862.1 cassette chromosome recombinase CcrA Machinery gene
  MUA81_RS00175 (MUA81_00175) cch1 37680..39470 (-) 1791 WP_262900864.1 cassette chromosome replicative helicase -
  MUA81_RS00180 (MUA81_00180) - 39595..41025 (-) 1431 WP_262900866.1 DUF6038 family protein -
  MUA81_RS00185 (MUA81_00185) - 41454..41831 (+) 378 WP_262900868.1 PD-(D/E)XK nuclease family protein -
  MUA81_RS00190 (MUA81_00190) - 41987..43840 (+) 1854 WP_262900869.1 B domain-containing protein -
  MUA81_RS00195 (MUA81_00195) - 44200..45513 (-) 1314 WP_262900870.1 ISL3 family transposase -
  MUA81_RS00200 (MUA81_00200) - 45725..45946 (-) 222 WP_262900871.1 hypothetical protein -
  MUA81_RS00205 (MUA81_00205) - 45961..46461 (-) 501 WP_262900872.1 DUF1643 domain-containing protein -
  MUA81_RS00210 (MUA81_00210) - 46475..46786 (-) 312 WP_011302036.1 DUF960 family protein -
  MUA81_RS00215 (MUA81_00215) - 46882..47220 (-) 339 WP_262900874.1 SAUGI family uracil-DNA glycosylase inhibitor -
  MUA81_RS00220 (MUA81_00220) ccrB 47694..49322 (-) 1629 Protein_41 cassette chromosome recombinase CcrB -
  MUA81_RS00225 (MUA81_00225) ccrA 49343..50692 (-) 1350 WP_262900875.1 cassette chromosome recombinase CcrA Machinery gene
  MUA81_RS00230 (MUA81_00230) - 50884..51162 (-) 279 WP_069854625.1 hypothetical protein -
  MUA81_RS00235 (MUA81_00235) cch1 51232..53031 (-) 1800 WP_262900876.1 cassette chromosome replicative helicase -
  MUA81_RS00240 (MUA81_00240) - 53158..54030 (-) 873 WP_262901383.1 DUF6038 family protein -
  MUA81_RS00245 (MUA81_00245) - 54148..60849 (+) 6702 WP_262900877.1 YSIRK-type signal peptide-containing protein -
  MUA81_RS00250 (MUA81_00250) - 61850..63913 (+) 2064 WP_262900878.1 heavy metal translocating P-type ATPase -
  MUA81_RS00255 (MUA81_00255) - 64041..64463 (+) 423 WP_316960040.1 YdhK family protein -
  MUA81_RS00260 (MUA81_00260) - 64760..65146 (+) 387 WP_262559937.1 hypothetical protein -
  MUA81_RS00265 (MUA81_00265) - 65171..65845 (+) 675 WP_262604229.1 IS6 family transposase -
  MUA81_RS00270 (MUA81_00270) - 66078..66743 (-) 666 WP_262900882.1 Cthe_2314 family HEPN domain-containing protein -
  MUA81_RS00275 (MUA81_00275) - 66979..67940 (+) 962 Protein_52 M23 family metallopeptidase -
  MUA81_RS00280 (MUA81_00280) - 68161..68487 (+) 327 WP_316960041.1 Rrf2 family transcriptional regulator -
  MUA81_RS00285 (MUA81_00285) merA 68474..69796 (+) 1323 WP_262900884.1 hypothiocyanous acid reductase MerA -
  MUA81_RS00290 (MUA81_00290) - 69976..70290 (+) 315 WP_037576813.1 metalloregulator ArsR/SmtB family transcription factor -
  MUA81_RS00295 (MUA81_00295) arsB 70293..71584 (+) 1292 Protein_56 arsenite efflux transporter membrane subunit ArsB -
  MUA81_RS00300 (MUA81_00300) arsC 71602..72022 (+) 421 Protein_57 arsenate reductase (thioredoxin) -
  MUA81_RS00305 (MUA81_00305) cch1 72121..73889 (-) 1769 Protein_58 cassette chromosome replicative helicase -
  MUA81_RS13340 ssb 73911..74198 (-) 288 Protein_59 cassette chromosome ssDNA-binding protein -
  MUA81_RS00310 (MUA81_00310) - 74462..75472 (-) 1011 WP_262900886.1 hypothetical protein -
  MUA81_RS00315 (MUA81_00315) - 75650..76009 (-) 360 WP_262900888.1 hypothetical protein -
  MUA81_RS00320 (MUA81_00320) - 76481..77173 (+) 693 WP_037576810.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  MUA81_RS00325 (MUA81_00325) - 77170..78723 (+) 1554 WP_250396297.1 ABC transporter substrate-binding protein -
  MUA81_RS00330 (MUA81_00330) - 78726..79682 (+) 957 WP_250396298.1 ABC transporter permease -
  MUA81_RS00335 (MUA81_00335) - 79682..80449 (+) 768 WP_262900890.1 ABC transporter permease -
  MUA81_RS00340 (MUA81_00340) - 80416..81183 (+) 768 WP_262900891.1 ATP-binding cassette domain-containing protein -
  MUA81_RS00345 (MUA81_00345) - 81176..81811 (+) 636 WP_262559965.1 ATP-binding cassette domain-containing protein -
  MUA81_RS00350 (MUA81_00350) - 82392..82961 (-) 570 Protein_68 IS30 family transposase -
  MUA81_RS00355 (MUA81_00355) - 82934..83272 (-) 339 WP_316960042.1 helix-turn-helix domain-containing protein -
  MUA81_RS00360 (MUA81_00360) cstB 83383..84717 (+) 1335 Protein_70 persulfide dioxygenase-sulfurtransferase CstB -
  MUA81_RS00370 (MUA81_00370) - 85481..86794 (-) 1314 WP_262901385.1 ISL3 family transposase -
  MUA81_RS00375 (MUA81_00375) - 86840..87070 (-) 231 Protein_72 hypothetical protein -
  MUA81_RS00380 (MUA81_00380) - 87081..87494 (-) 414 WP_252559391.1 universal stress protein -
  MUA81_RS00385 (MUA81_00385) - 87507..88958 (-) 1452 WP_252559394.1 SulP family inorganic anion transporter -
  MUA81_RS00390 (MUA81_00390) - 89063..89338 (+) 276 Protein_75 recombinase family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52306.05 Da        Isoelectric Point: 10.1337

>NTDB_id=670714 MUA81_RS00170 WP_262900862.1 36152..37501(-) (ccrA) [Staphylococcus simulans strain IVB6244]
MKQAIGYLRQSTTKQQSLGAQKQTIQTLAKKYNVQHITFYSDKQSGRTDNRTGYQQVIESIQQRQCDVLCCYRLNRLHRN
LKNALKLMKLCQKYHVHILSIHDGYFDMDKAFDRLKLNIFISLAELESDNIGEQVKNGLKEKARQGKLITTHAPFGYTYR
NGTFTINQDKAPTVKTIFHYYLQGYGYKKIAQYLEEDTKLINRKPYQVRAIIMNPNYCGRVINQYGQYDNMFPSIVSTNI
YEQAQEIRSQKHTKRTPSDNQLQKKIKCPYCGSTLTNMTIRKKHHTLRYYVCPKNMNASRFVCAFKGINAQVLETSVLAT
CQNFFQNQQLYSKINQTIQQRFKRQRDIEAKHTLTQEQLIDKLAKGMIDAETFRNQSQSVLQKRKTISSISDTQLQTSLQ
KVIQKSFTLNMLYPYIDEIHITKNKELSGIYLKDEPLNIVNQAVQSSTV

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=670714 MUA81_RS00170 WP_262900862.1 36152..37501(-) (ccrA) [Staphylococcus simulans strain IVB6244]
ATGAAACAAGCTATTGGTTATTTACGCCAAAGTACAACTAAGCAACAATCCCTAGGAGCACAGAAACAAACTATCCAAAC
ACTCGCTAAAAAATACAATGTCCAACACATCACCTTTTATAGTGATAAGCAATCAGGCAGAACGGATAATCGAACTGGTT
ATCAACAAGTCATCGAAAGCATTCAACAAAGACAATGTGACGTATTATGTTGTTATCGCTTGAATCGACTGCATCGTAAT
TTGAAAAATGCATTAAAACTTATGAAACTGTGTCAAAAGTATCATGTTCATATTCTAAGTATTCATGATGGCTATTTTGA
TATGGATAAAGCGTTTGATCGCCTAAAACTTAATATATTCATAAGTCTCGCAGAATTAGAGTCCGATAATATTGGTGAAC
AAGTCAAGAATGGTCTTAAAGAAAAAGCTAGACAAGGTAAACTCATCACGACACATGCACCATTTGGTTATACCTATCGC
AATGGCACATTCACAATTAATCAAGATAAAGCACCAACGGTTAAGACAATATTCCATTACTACCTACAAGGCTATGGTTA
TAAGAAAATTGCGCAATACTTAGAAGAAGATACTAAGCTAATCAACCGAAAACCCTATCAAGTACGCGCTATTATCATGA
ATCCTAATTATTGTGGTCGCGTTATCAATCAATATGGACAATATGATAACATGTTCCCATCTATTGTTTCGACCAATATA
TACGAACAAGCTCAAGAAATACGGTCACAAAAACATACAAAACGGACTCCCTCAGATAATCAACTTCAGAAGAAAATAAA
ATGTCCATATTGTGGATCAACCCTGACTAATATGACGATTAGAAAAAAGCACCATACCTTACGTTATTATGTCTGTCCTA
AAAACATGAATGCATCACGTTTTGTCTGTGCATTCAAAGGAATCAATGCGCAAGTATTAGAAACAAGTGTCTTAGCGACT
TGTCAGAACTTCTTTCAGAACCAACAGCTCTATTCAAAAATCAACCAAACAATTCAACAACGATTCAAAAGACAAAGGGA
CATAGAAGCTAAACATACACTCACGCAAGAACAACTGATAGATAAACTTGCCAAAGGTATGATTGATGCTGAAACATTCA
GGAATCAGTCTCAGTCAGTGCTTCAAAAGCGCAAAACCATATCTTCAATAAGTGATACTCAGTTACAAACATCACTACAA
AAGGTTATACAGAAAAGTTTCACGTTAAACATGCTGTATCCCTATATTGATGAAATTCATATTACTAAAAATAAAGAACT
AAGCGGCATCTATTTAAAAGATGAGCCACTAAACATTGTTAATCAAGCGGTACAATCATCAACTGTCTAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(169-250)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-322)

Resolvase, N terminal domain (Resolvase)

(4-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

80.624

100

0.806
  ccrA Staphylococcus aureus N315

73.154

99.555

0.728