Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MMY79_RS16805 Genome accession   NZ_CP094657
Coordinates   3625571..3626371 (-) Length   266 a.a.
NCBI ID   WP_252610406.1    Uniprot ID   -
Organism   Acinetobacter sp. XS-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3620571..3631371
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMY79_RS16785 (MMY79_16780) - 3621636..3622334 (-) 699 WP_004641116.1 tetratricopeptide repeat protein -
  MMY79_RS16790 (MMY79_16785) hisS 3622363..3623655 (-) 1293 WP_252610402.1 histidine--tRNA ligase -
  MMY79_RS16795 (MMY79_16790) ispG 3623652..3624767 (-) 1116 WP_206236569.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  MMY79_RS16800 (MMY79_16795) - 3624789..3625580 (-) 792 WP_252610404.1 helix-turn-helix domain-containing protein -
  MMY79_RS16805 (MMY79_16800) pilF 3625571..3626371 (-) 801 WP_252610406.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  MMY79_RS16810 (MMY79_16805) rlmN 3626386..3627621 (-) 1236 WP_252610408.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  MMY79_RS16815 (MMY79_16810) ndk 3627745..3628176 (-) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  MMY79_RS16820 (MMY79_16815) iscX 3628290..3628490 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  MMY79_RS16825 (MMY79_16820) - 3628590..3629141 (-) 552 WP_289781517.1 phosphatase PAP2 family protein -
  MMY79_RS16830 (MMY79_16825) - 3629166..3630455 (-) 1290 WP_252613555.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30035.31 Da        Isoelectric Point: 8.5713

>NTDB_id=670436 MMY79_RS16805 WP_252610406.1 3625571..3626371(-) (pilF) [Acinetobacter sp. XS-4]
MSTPKLKTVLCMGVAAALLVSGCQTTHMQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGTTLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQSLLANRDSCISMLELAEIFYLQQQIPAATQMYEQYVHTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSIEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=670436 MMY79_RS16805 WP_252610406.1 3625571..3626371(-) (pilF) [Acinetobacter sp. XS-4]
TTGAGTACTCCAAAGTTGAAAACGGTATTATGTATGGGGGTTGCAGCTGCACTTCTGGTAAGTGGTTGCCAGACAACGCA
TATGCAAAAAAAAGACCCAGAAAAAGCGGTAAAGGTACGTACACAACTTGCTGCTGAACATATCCGTTCTGGTGACTTGG
ATTCGGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
CTACAGCAAGAGGGTAGTAAACCTAACTTAGAAAAAGCAGAACATTATTTTAAACGGGCAATTTCATCTGAACCAGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATACCAAATGGAACGCTATAATGATGCGGTCGAGCAATTTCGCATTG
CTGGCACAACATTAGGTTATGACCAACGCTATCAGGCATTAGAGAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAATCACTCCTTGCTAACCGCGACTCTTGTATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCATACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTCTTAGTAAACCAG
TTACGTGCTTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTATTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-133)

Tetratricopeptide repeat (TPR_8)

(145-176)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

92.481

100

0.925
  pilF Acinetobacter baylyi ADP1

73.846

97.744

0.722