Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MP622_RS16580 Genome accession   NZ_CP094643
Coordinates   3490594..3491358 (+) Length   254 a.a.
NCBI ID   WP_016166210.1    Uniprot ID   -
Organism   Acinetobacter sp. PK01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3485594..3496358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MP622_RS16550 (MP622_16550) - 3485904..3486314 (+) 411 WP_016166216.1 hypothetical protein -
  MP622_RS16555 (MP622_16555) - 3486467..3487750 (+) 1284 WP_243791864.1 glycosyltransferase family 1 protein -
  MP622_RS16560 (MP622_16560) - 3487779..3488330 (+) 552 WP_044739035.1 phosphatase PAP2 family protein -
  MP622_RS16565 (MP622_16565) iscX 3488410..3488610 (+) 201 WP_016166213.1 Fe-S cluster assembly protein IscX -
  MP622_RS16570 (MP622_16570) ndk 3488737..3489168 (+) 432 WP_016166212.1 nucleoside-diphosphate kinase -
  MP622_RS16575 (MP622_16575) rlmN 3489292..3490527 (+) 1236 WP_243791866.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  MP622_RS16580 (MP622_16580) pilF 3490594..3491358 (+) 765 WP_016166210.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  MP622_RS16585 (MP622_16585) - 3491349..3492143 (+) 795 WP_016166209.1 helix-turn-helix domain-containing protein -
  MP622_RS16590 (MP622_16590) ispG 3492164..3493279 (+) 1116 WP_044739032.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  MP622_RS16595 (MP622_16595) hisS 3493276..3494571 (+) 1296 WP_243791867.1 histidine--tRNA ligase -
  MP622_RS16600 (MP622_16600) - 3494605..3495309 (+) 705 WP_243791869.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 28684.58 Da        Isoelectric Point: 6.9887

>NTDB_id=670346 MP622_RS16580 WP_016166210.1 3490594..3491358(+) (pilF) [Acinetobacter sp. PK01]
MSLVGCITTTTSSAPGFKKDPEKAVEVRTQLAAEYIRKGDLDAAKRALDQALEMEPRDSTANMMMGVLLQQEGSPANMEK
AETHFKRAISADPNNAQARNNYGTYLYVVERYNEAIEQLAIAGSTLGYDQRFKALENLGRIYLKLDDTANAEKSFKQALQ
VNPNSYVSMLELSEIFYLRQQIPAATQLYEQFVRLVGQKNQSARALWIGIRIARANGDQMGMQVLVNQMRALFPDSQEYT
RYLQLQYSTEAVWK

Nucleotide


Download         Length: 765 bp        

>NTDB_id=670346 MP622_RS16580 WP_016166210.1 3490594..3491358(+) (pilF) [Acinetobacter sp. PK01]
ATGTCGTTGGTGGGTTGTATTACCACAACCACCTCATCGGCACCGGGGTTTAAAAAAGACCCCGAGAAAGCAGTAGAAGT
GCGGACTCAATTGGCAGCGGAATATATTCGTAAGGGCGACCTAGATGCGGCAAAACGCGCACTTGATCAAGCATTAGAAA
TGGAACCACGAGATTCCACGGCGAATATGATGATGGGCGTGTTGCTACAGCAAGAAGGTAGCCCTGCCAATATGGAAAAA
GCGGAGACACACTTTAAACGTGCAATTTCTGCCGATCCAAATAATGCGCAAGCTCGTAACAACTATGGTACGTATTTATA
CGTCGTAGAACGTTATAATGAGGCGATTGAACAATTAGCAATTGCTGGTTCGACCTTGGGTTATGATCAGCGATTCAAGG
CATTGGAAAATTTAGGACGTATTTACTTAAAATTGGATGATACAGCAAATGCTGAAAAATCATTCAAGCAAGCATTGCAA
GTAAATCCAAATTCTTATGTTTCAATGTTAGAGTTGTCAGAAATATTTTATTTAAGACAACAAATTCCTGCTGCTACACA
GCTATATGAACAATTTGTTCGTTTGGTTGGGCAGAAAAATCAGAGTGCGCGTGCATTATGGATTGGTATTCGTATTGCAC
GAGCCAATGGCGATCAGATGGGGATGCAAGTGTTAGTCAATCAAATGCGAGCATTGTTCCCCGATAGCCAAGAATACACA
CGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_1)

(133-164)

Tetratricopeptide repeat (TPR_8)

(26-57)

Tetratricopeptide repeat (TPR_17)

(85-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

76.8

98.425

0.756
  pilF Acinetobacter baylyi ADP1

69.841

99.213

0.693