Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MST18_RS12970 Genome accession   NZ_CP094555
Coordinates   2714803..2715552 (-) Length   249 a.a.
NCBI ID   WP_373691629.1    Uniprot ID   -
Organism   Acinetobacter sp. YH12068_T     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2709803..2720552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MST18_RS12950 (MST18_12935) - 2710845..2711543 (-) 699 WP_078192660.1 tetratricopeptide repeat protein -
  MST18_RS12955 (MST18_12940) hisS 2711574..2712869 (-) 1296 WP_180005380.1 histidine--tRNA ligase -
  MST18_RS12960 (MST18_12945) ispG 2712873..2713994 (-) 1122 WP_078192661.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  MST18_RS12965 (MST18_12950) - 2714018..2714812 (-) 795 WP_180124989.1 RodZ domain-containing protein -
  MST18_RS12970 (MST18_12955) pilF 2714803..2715552 (-) 750 WP_373691629.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  MST18_RS12975 (MST18_12960) rlmN 2715622..2716857 (-) 1236 WP_180034883.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  MST18_RS12980 (MST18_12965) ndk 2716981..2717412 (-) 432 WP_004697669.1 nucleoside-diphosphate kinase -
  MST18_RS12985 (MST18_12970) iscX 2717522..2717722 (-) 201 WP_078189615.1 Fe-S cluster assembly protein IscX -
  MST18_RS12990 (MST18_12975) - 2717844..2718395 (-) 552 WP_180132660.1 phosphatase PAP2 family protein -
  MST18_RS12995 (MST18_12980) - 2718589..2719866 (-) 1278 WP_373686661.1 glycosyltransferase family 4 protein -
  MST18_RS13000 (MST18_12985) - 2720080..2720283 (-) 204 WP_180132662.1 ATP-binding protein -

Sequence

Protein


Download         Length: 249 a.a.        Molecular weight: 28099.74 Da        Isoelectric Point: 8.6162

>NTDB_id=670182 MST18_RS12970 WP_373691629.1 2714803..2715552(-) (pilF) [Acinetobacter sp. YH12068_T]
MALTACQTPEIGKKDPEKAVKIRTQLAVEHLKAGDLDSAKRALDQALEVDSRDSQANMMMGVLLQREGSPANLEKADSYF
KRAISADSKNSQARNNYGTYLYQVERYNDAIEQFSIAGSTLGYDQRFKALENLGRIYLKVGDVANAEKSFKQALQANRDS
YVSMLELSEIFYLNQQFPAATQMYEQFVRAVGQKNQGARALWIGIRTARANGDNLGMQVLVNQLRALFPESPEYQRYLQL
QYSTEAVWK

Nucleotide


Download         Length: 750 bp        

>NTDB_id=670182 MST18_RS12970 WP_373691629.1 2714803..2715552(-) (pilF) [Acinetobacter sp. YH12068_T]
TTGGCATTAACAGCATGTCAAACGCCTGAGATTGGGAAAAAAGACCCAGAGAAAGCCGTAAAAATCAGAACGCAATTGGC
TGTTGAACACCTGAAAGCAGGGGATTTGGACTCTGCTAAGCGCGCATTGGATCAGGCTTTAGAAGTGGATTCTCGCGATT
CCCAAGCCAATATGATGATGGGCGTCTTACTACAACGAGAAGGCAGTCCAGCCAACTTAGAAAAAGCAGATAGTTATTTT
AAGCGTGCAATTTCAGCAGACTCAAAAAATTCTCAAGCACGTAACAACTACGGTACGTATTTATACCAAGTTGAGCGTTA
TAATGATGCGATTGAACAGTTCAGCATCGCAGGTTCAACTTTAGGTTATGATCAACGTTTCAAAGCATTGGAAAATTTAG
GTCGAATTTACCTGAAAGTGGGTGATGTGGCGAATGCTGAAAAATCATTCAAACAAGCTTTGCAGGCTAATCGTGATTCC
TATGTTTCAATGTTAGAGTTGTCTGAAATCTTTTATTTGAATCAGCAGTTCCCTGCTGCTACACAAATGTATGAACAATT
TGTACGTGCAGTAGGACAAAAAAATCAGGGTGCGCGTGCACTCTGGATTGGCATTCGAACAGCGCGTGCCAATGGCGACA
ATTTGGGTATGCAAGTACTGGTCAATCAGTTACGTGCTTTGTTCCCAGAAAGTCCTGAATACCAACGCTATTTGCAATTA
CAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(128-159)

Tetratricopeptide repeat (TPR_16)

(25-64)

Tetratricopeptide repeat (TPR_16)

(72-115)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

80.488

98.795

0.795
  pilF Acinetobacter baylyi ADP1

73.279

99.197

0.727