Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   TAO_RS08885 Genome accession   NZ_AP014836
Coordinates   1889875..1891593 (+) Length   572 a.a.
NCBI ID   WP_096527569.1    Uniprot ID   A0A1Q2SPN0
Organism   Candidatus Nitrosoglobus terrae strain TAO100     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1884875..1896593
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TAO_RS08865 (TAO_1720) trxA 1885997..1886323 (+) 327 WP_096527565.1 thioredoxin TrxA -
  TAO_RS08870 (TAO_1721) - 1886682..1887416 (-) 735 WP_096527566.1 DUF484 family protein -
  TAO_RS08875 (TAO_1722) dapF 1887413..1888243 (-) 831 WP_096527567.1 diaminopimelate epimerase -
  TAO_RS08880 (TAO_1723) lysA 1888327..1889577 (-) 1251 WP_096527568.1 diaminopimelate decarboxylase -
  TAO_RS10010 lptM 1889581..1889706 (-) 126 WP_231910644.1 LPS translocon maturation chaperone LptM -
  TAO_RS08885 (TAO_1724) pilB 1889875..1891593 (+) 1719 WP_096527569.1 type IV-A pilus assembly ATPase PilB Machinery gene
  TAO_RS08890 (TAO_1725) pilC 1891598..1892818 (+) 1221 WP_096527570.1 type II secretion system F family protein Machinery gene
  TAO_RS08895 (TAO_1726) pilD 1892849..1893718 (+) 870 WP_096527571.1 A24 family peptidase Machinery gene
  TAO_RS08900 (TAO_1727) coaE 1893743..1894348 (+) 606 Protein_1760 dephospho-CoA kinase -
  TAO_RS09690 yacG 1894370..1894561 (+) 192 WP_172419115.1 DNA gyrase inhibitor YacG -
  TAO_RS08905 (TAO_1728) - 1894567..1895535 (-) 969 WP_096527573.1 Nudix family hydrolase -
  TAO_RS08910 (TAO_1729) - 1895569..1895769 (+) 201 WP_096527817.1 DUF2905 domain-containing protein -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 63358.82 Da        Isoelectric Point: 6.3829

>NTDB_id=67016 TAO_RS08885 WP_096527569.1 1889875..1891593(+) (pilB) [Candidatus Nitrosoglobus terrae strain TAO100]
MLTPQTQTITPGLVRLLVKEGLLTQEAAPQHYEQSRKAGTSFVSYLIQQKLVESRNISQVISREFGIPLFDLDTLDLNYL
PKGLIEEKLLIQHRALPIFKRGNRLFVALADPTNLQALSEIKFHTGINTEAIIVEEDKLTRTIEQITKKQDTSLAELHDI
VLDSLETPSSEDESSGITIDPSVDDTPIVRFINQALLDAINRGASDIHFEPYEKSYRIRYRQDGILHEVSSPPIALATRI
AARIKVMARLDISERRIPQDGRMKMKISKQKAIDFRVSTCPTLFGEKIVLRILDPTSAQLGIEALGYEENQKNIFLETIH
RPYGMVLVTGPTGSGKTVSLYTALNILNTGDRNISTAEDPAEINLPGINQVNIYPKVGLTFAGTLRAFLRQDPDVIMVGE
IRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTLTRLSNMGVASYNIASAVSLIIAQRLIRRLCVHCKTPHEVPDETLLEE
GFTEAQINASPMVYKASSDCQHCSGGYKGRIGIYQVMPVSEKMGQMIMAGGNAIELAQQAKKEGITDLRQSGIKKVIEGI
TSLDEINRVTKE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=67016 TAO_RS08885 WP_096527569.1 1889875..1891593(+) (pilB) [Candidatus Nitrosoglobus terrae strain TAO100]
ATGTTAACTCCACAAACACAAACGATTACCCCCGGTCTCGTCCGTCTATTAGTCAAAGAAGGACTATTGACTCAGGAAGC
CGCACCCCAGCATTACGAGCAATCACGCAAAGCAGGCACTTCTTTTGTTTCCTATCTCATACAACAAAAATTAGTTGAAA
GCCGAAATATTAGCCAAGTGATCTCTCGAGAATTTGGTATTCCTTTATTTGATCTTGACACCTTAGACTTAAATTATCTT
CCTAAAGGACTCATAGAAGAAAAGCTCCTGATCCAACATCGGGCACTACCTATATTTAAGCGGGGGAATCGCCTATTTGT
GGCGTTAGCCGATCCTACCAATTTACAAGCACTAAGCGAGATTAAATTTCATACCGGTATTAATACCGAGGCTATTATCG
TTGAAGAAGATAAGCTTACTCGAACTATTGAACAGATTACAAAAAAACAAGATACCTCATTAGCGGAGCTGCACGATATC
GTCCTAGATAGCCTAGAGACTCCCTCAAGTGAGGATGAGTCATCTGGAATTACCATTGATCCCAGTGTTGATGATACCCC
CATAGTGCGCTTTATTAATCAAGCCCTGTTAGATGCTATTAACCGAGGGGCTTCAGATATCCATTTTGAGCCCTATGAAA
AAAGTTATCGCATCAGGTATCGGCAAGATGGGATATTACACGAAGTCTCATCCCCACCTATAGCGCTAGCCACTCGGATA
GCTGCACGTATTAAAGTGATGGCCAGATTAGATATTTCAGAACGCCGTATTCCTCAAGATGGGCGCATGAAAATGAAAAT
CTCTAAGCAGAAAGCCATTGATTTTCGAGTCAGCACTTGCCCTACCCTATTTGGCGAAAAAATTGTCCTCCGTATTTTAG
ATCCTACGAGTGCACAGCTAGGGATCGAAGCCTTGGGCTATGAGGAAAATCAAAAGAATATCTTCCTAGAAACCATTCAT
CGGCCTTATGGTATGGTACTGGTCACTGGACCCACGGGTAGCGGTAAAACCGTCTCTCTTTATACCGCTCTTAATATTCT
GAATACCGGAGATCGGAATATATCTACAGCTGAAGATCCCGCTGAAATCAATTTACCCGGTATTAATCAGGTTAATATCT
ACCCAAAAGTAGGCTTAACCTTTGCTGGCACCCTAAGAGCTTTTTTGCGCCAAGATCCAGACGTTATTATGGTTGGTGAG
ATTCGAGATCTTGAAACCGCTGAAATTGCCATTAAAGCAGCGCAAACCGGCCATATGGTGCTCTCCACCCTTCATACTAA
CGACGCGCCTCAAACCCTCACTCGGCTTTCAAACATGGGGGTAGCTTCTTACAATATCGCCTCAGCTGTCTCCCTTATTA
TTGCCCAGCGCTTGATACGGCGCCTTTGTGTTCATTGTAAAACTCCCCATGAAGTACCTGATGAAACCCTCTTAGAAGAA
GGTTTCACCGAAGCTCAGATTAACGCTAGCCCAATGGTCTATAAAGCTTCCTCTGACTGCCAGCATTGCTCAGGAGGCTA
TAAAGGTCGCATAGGTATTTATCAAGTGATGCCTGTATCAGAAAAGATGGGGCAGATGATTATGGCGGGTGGTAATGCGA
TAGAGTTGGCCCAGCAAGCAAAAAAAGAGGGCATTACTGATCTACGGCAATCAGGGATTAAAAAAGTCATCGAGGGCATT
ACCAGCTTAGATGAAATCAATCGAGTAACTAAGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Q2SPN0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

56.028

98.601

0.552
  pilB Acinetobacter baumannii D1279779

54.885

98.427

0.54
  pilB Acinetobacter baylyi ADP1

54.902

98.077

0.538
  pilF Neisseria gonorrhoeae MS11

51.056

99.301

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

49.11

98.252

0.483
  pilB Vibrio cholerae strain A1552

50.366

95.455

0.481
  pilB Vibrio campbellii strain DS40M4

48.932

98.252

0.481
  pilB/pilB1 Synechocystis sp. PCC 6803

36.111

100

0.386
  pilF Thermus thermophilus HB27

38.036

97.902

0.372

Multiple sequence alignment