Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MST17_RS13280 Genome accession   NZ_CP094545
Coordinates   2717961..2718710 (-) Length   249 a.a.
NCBI ID   WP_373691629.1    Uniprot ID   -
Organism   Acinetobacter sp. YH16056_T     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2712961..2723710
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MST17_RS13260 (MST17_13240) - 2714003..2714701 (-) 699 WP_078189608.1 tetratricopeptide repeat protein -
  MST17_RS13265 (MST17_13245) hisS 2714732..2716027 (-) 1296 WP_180005380.1 histidine--tRNA ligase -
  MST17_RS13270 (MST17_13250) ispG 2716031..2717152 (-) 1122 WP_180007218.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  MST17_RS13275 (MST17_13255) - 2717176..2717970 (-) 795 WP_180025979.1 RodZ domain-containing protein -
  MST17_RS13280 (MST17_13260) pilF 2717961..2718710 (-) 750 WP_373691629.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  MST17_RS13285 (MST17_13265) rlmN 2718780..2720015 (-) 1236 WP_078189613.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  MST17_RS13290 (MST17_13270) ndk 2720139..2720570 (-) 432 WP_078189614.1 nucleoside-diphosphate kinase -
  MST17_RS13295 (MST17_13275) iscX 2720680..2720880 (-) 201 WP_078189615.1 Fe-S cluster assembly protein IscX -
  MST17_RS13300 (MST17_13280) - 2721002..2721553 (-) 552 WP_180025980.1 phosphatase PAP2 family protein -
  MST17_RS13305 (MST17_13285) - 2721752..2723023 (-) 1272 WP_373685438.1 glycosyltransferase family 4 protein -
  MST17_RS13310 (MST17_13290) - 2723143..2723427 (-) 285 WP_168378455.1 sensor histidine kinase -

Sequence

Protein


Download         Length: 249 a.a.        Molecular weight: 28099.74 Da        Isoelectric Point: 8.6162

>NTDB_id=670145 MST17_RS13280 WP_373691629.1 2717961..2718710(-) (pilF) [Acinetobacter sp. YH16056_T]
MALTACQTPEIGKKDPEKAVKIRTQLAVEHLKAGDLDSAKRALDQALEVDSRDSQANMMMGVLLQREGSPANLEKADSYF
KRAISADSKNSQARNNYGTYLYQVERYNDAIEQFSIAGSTLGYDQRFKALENLGRIYLKVGDVANAEKSFKQALQANRDS
YVSMLELSEIFYLNQQFPAATQMYEQFVRAVGQKNQGARALWIGIRTARANGDNLGMQVLVNQLRALFPESPEYQRYLQL
QYSTEAVWK

Nucleotide


Download         Length: 750 bp        

>NTDB_id=670145 MST17_RS13280 WP_373691629.1 2717961..2718710(-) (pilF) [Acinetobacter sp. YH16056_T]
TTGGCATTAACAGCATGTCAAACGCCTGAGATTGGGAAAAAAGACCCAGAGAAAGCCGTAAAAATCAGAACGCAATTGGC
TGTTGAACACCTGAAAGCAGGGGATTTGGACTCTGCTAAACGTGCATTAGATCAGGCTTTAGAGGTGGATTCTCGCGATT
CCCAAGCCAATATGATGATGGGTGTCTTACTACAACGAGAAGGCAGTCCAGCCAACTTAGAAAAAGCAGATAGTTATTTT
AAGCGTGCAATTTCAGCAGACTCAAAAAATTCTCAAGCACGTAACAACTACGGTACGTATTTATACCAAGTTGAGCGTTA
TAATGATGCGATTGAACAGTTCAGCATCGCAGGTTCAACTTTAGGTTATGATCAACGTTTCAAAGCATTGGAAAATTTAG
GTCGAATTTACCTGAAAGTGGGTGATGTGGCGAATGCTGAAAAATCATTCAAACAAGCTTTGCAGGCTAATCGTGATTCC
TATGTTTCAATGTTAGAGTTGTCTGAAATCTTTTATTTGAATCAGCAGTTCCCTGCTGCCACACAAATGTATGAACAATT
TGTACGTGCAGTAGGACAAAAAAATCAGGGTGCGCGTGCACTCTGGATTGGCATTCGAACAGCGCGTGCCAATGGCGACA
ATTTGGGTATGCAAGTACTGGTTAACCAGTTACGTGCTTTGTTCCCAGAAAGTCCTGAATACCAACGCTATTTGCAATTA
CAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(128-159)

Tetratricopeptide repeat (TPR_16)

(25-64)

Tetratricopeptide repeat (TPR_16)

(72-115)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

80.488

98.795

0.795
  pilF Acinetobacter baylyi ADP1

73.279

99.197

0.727