Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MST16_RS02840 Genome accession   NZ_CP094541
Coordinates   597341..598090 (+) Length   249 a.a.
NCBI ID   WP_373691629.1    Uniprot ID   -
Organism   Acinetobacter sp. YH16040_T     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 592341..603090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MST16_RS02810 (MST16_02805) - 592437..592910 (+) 474 WP_180011662.1 sensor histidine kinase -
  MST16_RS02815 (MST16_02810) - 593030..594307 (+) 1278 WP_373683470.1 glycosyltransferase family 4 protein -
  MST16_RS02820 (MST16_02815) - 594498..595049 (+) 552 WP_180011666.1 phosphatase PAP2 family protein -
  MST16_RS02825 (MST16_02820) iscX 595171..595371 (+) 201 WP_078189615.1 Fe-S cluster assembly protein IscX -
  MST16_RS02830 (MST16_02825) ndk 595481..595912 (+) 432 WP_078189614.1 nucleoside-diphosphate kinase -
  MST16_RS02835 (MST16_02830) rlmN 596036..597271 (+) 1236 WP_078189613.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  MST16_RS02840 (MST16_02835) pilF 597341..598090 (+) 750 WP_373691629.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  MST16_RS02845 (MST16_02840) - 598081..598875 (+) 795 WP_111884744.1 RodZ domain-containing protein -
  MST16_RS02850 (MST16_02845) ispG 598899..600020 (+) 1122 WP_180011668.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  MST16_RS02855 (MST16_02850) hisS 600024..601319 (+) 1296 WP_180011670.1 histidine--tRNA ligase -
  MST16_RS02860 (MST16_02855) - 601350..602048 (+) 699 WP_078189608.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 249 a.a.        Molecular weight: 28099.74 Da        Isoelectric Point: 8.6162

>NTDB_id=670082 MST16_RS02840 WP_373691629.1 597341..598090(+) (pilF) [Acinetobacter sp. YH16040_T]
MALTACQTPEIGKKDPEKAVKIRTQLAVEHLKAGDLDSAKRALDQALEVDSRDSQANMMMGVLLQREGSPANLEKADSYF
KRAISADSKNSQARNNYGTYLYQVERYNDAIEQFSIAGSTLGYDQRFKALENLGRIYLKVGDVANAEKSFKQALQANRDS
YVSMLELSEIFYLNQQFPAATQMYEQFVRAVGQKNQGARALWIGIRTARANGDNLGMQVLVNQLRALFPESPEYQRYLQL
QYSTEAVWK

Nucleotide


Download         Length: 750 bp        

>NTDB_id=670082 MST16_RS02840 WP_373691629.1 597341..598090(+) (pilF) [Acinetobacter sp. YH16040_T]
TTGGCATTAACAGCATGTCAAACGCCTGAGATTGGGAAAAAAGACCCAGAGAAAGCCGTAAAAATCAGAACGCAATTGGC
TGTTGAACACCTGAAAGCAGGGGATTTGGACTCTGCTAAACGTGCATTAGATCAGGCTTTAGAGGTGGATTCTCGCGATT
CCCAAGCCAATATGATGATGGGTGTCTTACTACAACGAGAAGGCAGTCCAGCCAACTTAGAAAAAGCAGATAGTTATTTT
AAGCGTGCAATTTCAGCAGACTCAAAAAATTCTCAAGCACGTAACAACTACGGTACGTATTTATATCAAGTTGAGCGCTA
TAATGATGCGATTGAACAGTTCAGCATCGCAGGTTCAACTTTAGGTTATGATCAACGTTTCAAAGCATTGGAAAATTTAG
GTCGAATTTACCTGAAAGTGGGTGATGTGGCGAATGCTGAAAAATCATTCAAACAAGCTTTGCAGGCTAATCGTGATTCC
TATGTTTCAATGTTAGAGTTGTCTGAAATCTTTTATTTGAATCAGCAGTTCCCTGCTGCCACACAAATGTATGAACAATT
TGTACGTGCAGTAGGACAAAAAAATCAGGGTGCGCGTGCACTCTGGATTGGCATTCGAACAGCGCGTGCCAATGGCGACA
ATTTGGGTATGCAAGTACTGGTTAACCAGTTACGTGCTTTGTTCCCAGAAAGTCCTGAATACCAACGCTATTTGCAATTA
CAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(128-159)

Tetratricopeptide repeat (TPR_16)

(25-64)

Tetratricopeptide repeat (TPR_16)

(72-115)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

80.488

98.795

0.795
  pilF Acinetobacter baylyi ADP1

73.279

99.197

0.727