Detailed information    

experimental Experimentally validated

Overview

Name   MMJJ_11090   Type   Unclear
Locus tag   MMJJ_RS05500 Genome accession   NZ_CP026606
Coordinates   1027332..1029125 (-) Length   597 a.a.
NCBI ID   WP_104838005.1    Uniprot ID   A0A7J9PIE3
Organism   Methanococcus maripaludis strain DSM 2067     
Function   require for natural transformation   
Unclear

Function

In-frame deletion mutants of MMJJ_11090 were completely defective for transformation, and in trans expression under the control of the high expression Phmv promoter, rescued the transformation defects.


Genomic Context

Location: 1022332..1034125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMJJ_RS05490 (MMJJ_11070) hisF 1025682..1026500 (-) 819 WP_104838004.1 imidazole glycerol phosphate synthase subunit HisF -
  MMJJ_RS05495 (MMJJ_11080) MMJJ_11080 1026532..1027320 (-) 789 WP_011171665.1 MinD/ParA family ATP-binding protein Unclear
  MMJJ_RS05500 (MMJJ_11090) MMJJ_11090 1027332..1029125 (-) 1794 WP_104838005.1 hypothetical protein Unclear
  MMJJ_RS05505 (MMJJ_11100) - 1029320..1031974 (+) 2655 WP_104838006.1 hypothetical protein -
  MMJJ_RS05510 (MMJJ_11110) - 1032146..1032463 (+) 318 WP_048064150.1 helix-turn-helix domain-containing protein -
  MMJJ_RS05515 (MMJJ_11120) - 1032468..1033550 (+) 1083 WP_104838007.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 597 a.a.        Molecular weight: 68082.34 Da        Isoelectric Point: 4.0004

>NTDB_id=67 MMJJ_RS05500 WP_104838005.1 1027332..1029125(-) (MMJJ_11090) [Methanococcus maripaludis strain DSM 2067]
MKLKFFGLIIVSFMLLGVVNAIPYKWTSTVPITYVDISNIQDTEKLDYYLNSDNIVVIYTGNDFAMTPKTEAFLEYLGLV
KSKNIVSSIPDFGNSTFVNSNSEVIPASYVYRDENGAYLYINSNDAAEKDTTIVYEASISSKIIDDGYYEIKPGKLFIPY
NIDLENQDIVDFIGKYVALQGETFAYINKVPSNYDSVVINGIVVQNIIPDDNGESSISVADRKITVEVRDEDTINRKLAG
VKAASEILGIGTTYITTGSENIDIIKDSSITDDEKESLLNSYWFKKHYSDYYSHIKYNNYENYPNTQDLLAMSYYPLIYM
DKAPETFQDDPTGGYYPDTISYTGSESLGYWNEGAGSENSYYHYDGEDYWNDDETVTRDYWSVSGDFASMSEEIDFNDRY
EYFEHWYVKNYAYAMTGGANGLLLFSSDDDLLDAVFNKYNKNITWKLNLEGIDYVVIPDYIGYDKIDGIAVIKIPGLNNN
EINGMHFIDEIYIEPQNEEYGIYVADVYEYSTNTLYDLNDNNTWICSFEEYAKWAENYKTSRIYIKNDTVYVNADENAKI
TIYKENITRVTGTSTELYDATGKLIIAKAPDTISLSN

Nucleotide


Download         Length: 1794 bp        

>NTDB_id=67 MMJJ_RS05500 WP_104838005.1 1027332..1029125(-) (MMJJ_11090) [Methanococcus maripaludis strain DSM 2067]
ATGAAGCTGAAATTTTTTGGCTTGATTATTGTGTCATTTATGTTATTGGGGGTCGTAAACGCAATACCTTACAAGTGGAC
TTCGACAGTACCAATTACTTATGTTGATATTTCAAACATACAAGATACAGAAAAGCTTGATTACTATTTGAATTCGGATA
ATATTGTGGTAATATATACCGGAAACGATTTTGCAATGACTCCAAAAACAGAGGCATTTTTAGAATACCTGGGGCTTGTT
AAGTCAAAAAATATCGTTAGTAGTATTCCAGATTTTGGAAATTCTACGTTTGTAAATTCAAATTCGGAAGTTATTCCTGC
AAGTTATGTTTACAGAGACGAAAATGGTGCATACCTTTACATTAATTCAAACGATGCGGCTGAAAAAGATACAACAATAG
TATATGAAGCATCAATCAGTTCAAAAATCATTGATGATGGATACTACGAAATTAAACCTGGAAAATTATTTATTCCATAC
AATATCGACCTTGAAAATCAGGACATTGTTGATTTTATTGGAAAATATGTGGCACTTCAGGGTGAAACTTTTGCATATAT
CAATAAAGTTCCTTCAAATTACGATTCTGTTGTGATTAATGGAATTGTTGTACAAAATATTATTCCTGATGATAACGGGG
AAAGTTCAATTTCTGTTGCAGATCGAAAAATAACCGTTGAAGTGCGGGATGAAGATACAATTAATAGAAAGCTAGCAGGT
GTTAAGGCTGCTTCTGAAATTCTTGGAATTGGAACCACCTATATTACAACAGGTTCTGAAAATATTGATATAATAAAAGA
CAGTTCCATAACGGACGATGAAAAAGAAAGCCTTTTAAACAGCTATTGGTTTAAAAAACACTATTCTGATTATTATTCCC
ACATAAAATATAATAATTACGAAAATTACCCAAATACTCAAGATTTACTTGCAATGAGTTATTATCCATTAATATATATG
GATAAAGCTCCCGAAACATTCCAAGATGACCCAACCGGAGGATACTATCCAGATACAATTTCATATACGGGTTCTGAAAG
CCTCGGATACTGGAATGAAGGTGCAGGTTCTGAAAACAGTTATTACCACTACGATGGTGAAGACTACTGGAACGACGATG
AAACAGTTACCCGCGATTACTGGTCAGTCAGTGGCGATTTTGCAAGTATGAGCGAAGAAATAGACTTTAACGATAGATAC
GAATACTTTGAACATTGGTACGTTAAAAATTACGCTTATGCAATGACTGGTGGTGCAAACGGCCTTTTATTATTCTCGAG
TGATGACGATTTATTGGATGCGGTATTCAATAAATACAATAAGAATATTACTTGGAAATTGAACCTTGAAGGAATCGACT
ACGTGGTTATTCCTGATTATATCGGATACGATAAGATCGACGGAATTGCGGTTATTAAAATTCCTGGATTAAATAACAAC
GAAATAAATGGAATGCATTTTATTGATGAAATCTACATCGAACCACAAAATGAAGAATACGGAATTTACGTTGCAGACGT
TTATGAATACAGTACTAATACACTATATGATTTGAATGACAATAATACTTGGATTTGTTCATTTGAAGAATACGCAAAAT
GGGCTGAAAACTATAAAACCAGCAGAATATATATTAAAAATGATACTGTATACGTAAATGCAGACGAAAACGCAAAAATA
ACGATTTATAAAGAAAATATAACTCGTGTAACAGGTACCTCAACCGAGTTATACGATGCAACTGGAAAGTTGATTATAGC
AAAAGCACCAGATACAATTTCACTTAGTAACTAG

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7J9PIE3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Dallas R Fonseca et al. (2023) Random transposon mutagenesis identifies genes essential for transformation in Methanococcus maripaludis. Molecular Genetics And Genomics : MGG 298(3):537-548. [PMID: 36823423]