Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MRS60_RS14890 Genome accession   NZ_CP094459
Coordinates   3220535..3222043 (+) Length   502 a.a.
NCBI ID   WP_217590808.1    Uniprot ID   -
Organism   Burkholderia pyrrocinia strain JK-SH007     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3215535..3227043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRS60_RS14865 (MRS60_14865) kdsD 3216778..3217761 (+) 984 WP_105393307.1 arabinose 5-phosphate isomerase KdsD -
  MRS60_RS14870 (MRS60_14870) - 3217761..3218297 (+) 537 WP_034179056.1 HAD family hydrolase -
  MRS60_RS14875 (MRS60_14875) lptC 3218301..3218915 (+) 615 WP_034179057.1 LPS export ABC transporter periplasmic protein LptC -
  MRS60_RS14880 (MRS60_14880) lptA 3218936..3219601 (+) 666 WP_034179058.1 lipopolysaccharide transport periplasmic protein LptA -
  MRS60_RS14885 (MRS60_14885) lptB 3219598..3220374 (+) 777 WP_034179059.1 LPS export ABC transporter ATP-binding protein -
  MRS60_RS14890 (MRS60_14890) rpoN/rpoN1 3220535..3222043 (+) 1509 WP_217590808.1 RNA polymerase factor sigma-54 Machinery gene
  MRS60_RS14895 (MRS60_14895) raiA 3222203..3222562 (+) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  MRS60_RS14900 (MRS60_14900) - 3222759..3223271 (+) 513 WP_034195643.1 PTS sugar transporter subunit IIA -
  MRS60_RS14905 (MRS60_14905) hprK 3223401..3224369 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  MRS60_RS14910 (MRS60_14910) rapZ 3224445..3225353 (+) 909 WP_034179062.1 RNase adapter RapZ -
  MRS60_RS14915 (MRS60_14915) - 3225381..3226016 (+) 636 WP_034179063.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 54931.04 Da        Isoelectric Point: 4.8740

>NTDB_id=669679 MRS60_RS14890 WP_217590808.1 3220535..3222043(+) (rpoN/rpoN1) [Burkholderia pyrrocinia strain JK-SH007]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIVSPLRVAADGSLIAQTPSSQNTEPAP
ANSSSSTSGDRAERDEPAGADEYDGYASGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAS
PRDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRT
LALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVV
PRLRINNLYANILRNSRGDPSTGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=669679 MRS60_RS14890 WP_217590808.1 3220535..3222043(+) (rpoN/rpoN1) [Burkholderia pyrrocinia strain JK-SH007]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGTGAGCCCGCTGCGCGTCGCGGCGGACGGATCGCTGATCGCGCAGACACCTTCCAGCCAGAATACCGAGCCGGCCCCG
GCAAACAGCAGCAGCAGCACGTCAGGCGATCGCGCGGAGCGCGACGAGCCGGCCGGCGCGGACGAATACGACGGTTACGC
GTCCGGCGACGGCAACGACGGCCCGCAATGGAATCTCGACGAATTCGGCCGCTCGAGCGGCGCGTCCGACGACGACGACC
TCCCGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCAGC
CCGCGCGATCGTGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGT
GCTCGCCGACCTGCCGCCCGAGCTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACC
CGGCCGGGGTCGGCGCCCGCTCGGCCTCCGAATGCCTGAAGCTGCAGCTGCTGCGCCTCGATCCGTCCCCGACGCGCACG
CTCGCGCTCGAGATCGTGTCGCAGCATCTGGAGCTGCTCGCCGCACGCGACTTCACGCGATTGCGCAAGCACCTGAAGGC
GAGCGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCG
AGGCCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTG
CCGCGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGACCGGTTCGCTGAAACA
GCAGCTTCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCTATTG
TCGAGCGTCAAAAGAATTTCTTTGCACATGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTA
CTTCTTCGGATCTCACGTTTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGA
TAGGAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.317

100

0.687