Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MQW34_RS20040 Genome accession   NZ_CP094455
Coordinates   3912995..3913429 (-) Length   144 a.a.
NCBI ID   WP_000811492.1    Uniprot ID   A0A0G8EA73
Organism   Bacillus sp. ZJS3     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3907995..3918429
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MQW34_RS20025 (MQW34_20055) - 3908764..3909321 (+) 558 WP_078418999.1 PadR family transcriptional regulator -
  MQW34_RS20030 (MQW34_20065) metE 3910243..3912531 (+) 2289 WP_243498260.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  MQW34_RS20035 (MQW34_20070) comJ 3912569..3912958 (-) 390 WP_078418997.1 competence protein ComJ -
  MQW34_RS20040 (MQW34_20075) nucA/comI 3912995..3913429 (-) 435 WP_000811492.1 DNA-entry nuclease Machinery gene
  MQW34_RS20045 (MQW34_20080) - 3913666..3914415 (+) 750 WP_000388464.1 DUF3967 domain-containing protein -
  MQW34_RS20050 (MQW34_20085) kinB 3914468..3915742 (-) 1275 WP_243498262.1 sporulation sensor histidine kinase KinB -
  MQW34_RS20055 (MQW34_20090) - 3916053..3916253 (+) 201 WP_000929284.1 hypothetical protein -
  MQW34_RS20060 (MQW34_20095) - 3916378..3917541 (+) 1164 WP_078418995.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16012.35 Da        Isoelectric Point: 8.4331

>NTDB_id=669647 MQW34_RS20040 WP_000811492.1 3912995..3913429(-) (nucA/comI) [Bacillus sp. ZJS3]
MKQLKGIIISIIAILSILVAVYEVLVPEETSTKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=669647 MQW34_RS20040 WP_000811492.1 3912995..3913429(-) (nucA/comI) [Bacillus sp. ZJS3]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTTTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTACAAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGAGATATCCAGAAACAGGGA
AACATATTACAGATGCGATAAAGGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGCGCTGCGGATAGAAGAAAG
TTATCATTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATCGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGGTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8EA73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

70.796

78.472

0.556

Multiple sequence alignment