Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   L3476_RS24145 Genome accession   NZ_CP094446
Coordinates   5355721..5357088 (+) Length   455 a.a.
NCBI ID   WP_272826393.1    Uniprot ID   -
Organism   Paenibacillus thiaminolyticus strain Mbale2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5350721..5362088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3476_RS24125 (L3476_24065) - 5350764..5351228 (+) 465 WP_272826389.1 CtsR family transcriptional regulator -
  L3476_RS24130 (L3476_24070) - 5351327..5351890 (+) 564 WP_272826390.1 UvrB/UvrC motif-containing protein -
  L3476_RS24135 (L3476_24075) - 5351950..5353035 (+) 1086 WP_272826391.1 protein arginine kinase -
  L3476_RS24140 (L3476_24080) clpC 5353038..5355476 (+) 2439 WP_143802319.1 ATP-dependent protease ATP-binding subunit ClpC -
  L3476_RS24145 (L3476_24085) radA 5355721..5357088 (+) 1368 WP_272826393.1 DNA repair protein RadA Machinery gene
  L3476_RS24150 (L3476_24090) disA 5357105..5358178 (+) 1074 WP_087444208.1 DNA integrity scanning diadenylate cyclase DisA -
  L3476_RS24155 (L3476_24095) pssA 5358233..5358961 (+) 729 WP_087444209.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  L3476_RS24160 (L3476_24100) - 5359078..5359473 (-) 396 WP_087444210.1 DUF1573 domain-containing protein -
  L3476_RS24165 (L3476_24105) - 5359676..5360767 (+) 1092 WP_143802317.1 PIN/TRAM domain-containing protein -
  L3476_RS24170 (L3476_24110) ispD 5360810..5361523 (+) 714 WP_143802316.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  L3476_RS24175 (L3476_24115) ispF 5361557..5362039 (+) 483 WP_006676467.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49254.75 Da        Isoelectric Point: 6.4025

>NTDB_id=669593 L3476_RS24145 WP_272826393.1 5355721..5357088(+) (radA) [Paenibacillus thiaminolyticus strain Mbale2]
MAKVKTKFFCTDCGYESPKWMGKCPGCGEWNTMVEERETVVKTAGVHTSLIGTKEKPQSIIQIESKQEARLSTTIDELNR
VLGGGLVPGSLILVGGDPGIGKSTLLLQASNALAVSGLKVLYVSGEESTRQTKLRADRLGVLSERLFVLSETNLQYIEEA
TEAMSPDFLVIDSIQTVYQPSVESAPGSVAQVRECTSVCMRIAKGRGIATVLVGHVTKEGAISGPRMLEHMVDCVLYFEG
ERHHSYRILRAVKNRFGSTNEIGIFEMTEGGLTEVKNPSEMFLQERPLGVSGSTVVASLEGTRPVLVEMQALVSATNFPS
PRRMSTGIDHQRLSLIIAVLEKRMGMFLQNQDAYVNVAGGLKLDEPAVDLAIAVALASSFRDMPTQAFDVVFGEVGLTGE
VRAVSRAEQRVKEAEKLGFKRIILPEKSLRGWTPSGHIEIVGVNTVAEALTAALG

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=669593 L3476_RS24145 WP_272826393.1 5355721..5357088(+) (radA) [Paenibacillus thiaminolyticus strain Mbale2]
ATGGCCAAAGTAAAAACCAAGTTTTTCTGTACGGATTGCGGATATGAATCGCCGAAGTGGATGGGCAAATGCCCGGGCTG
CGGCGAGTGGAACACGATGGTGGAGGAGCGGGAGACGGTCGTCAAGACGGCGGGCGTCCACACATCCCTCATTGGGACGA
AAGAAAAACCGCAATCGATCATACAAATAGAAAGTAAACAGGAAGCGCGCCTGTCCACGACGATTGACGAATTGAACCGT
GTGCTCGGCGGCGGCCTGGTGCCGGGCTCGCTCATCCTCGTGGGCGGTGACCCGGGCATCGGCAAATCGACGCTGCTGCT
CCAGGCGTCCAATGCGCTGGCAGTCAGCGGCTTGAAGGTGCTGTACGTGTCTGGCGAGGAATCGACGCGCCAGACGAAGC
TGCGCGCCGACCGGCTCGGGGTGCTGTCCGAGCGTCTGTTCGTCCTGAGCGAGACGAATTTGCAGTATATTGAAGAGGCG
ACCGAGGCCATGAGCCCCGATTTTCTCGTCATCGACTCGATCCAGACGGTATATCAGCCGTCGGTCGAATCGGCTCCCGG
CAGCGTGGCTCAAGTTCGCGAATGCACAAGCGTGTGCATGCGCATTGCGAAGGGAAGAGGCATCGCGACCGTCCTCGTCG
GCCATGTAACGAAGGAAGGAGCCATTTCCGGCCCCCGCATGCTGGAGCATATGGTGGACTGCGTGCTTTATTTCGAAGGC
GAGCGCCATCATTCGTACCGCATCCTGCGGGCCGTGAAGAACCGCTTCGGTTCCACGAACGAGATCGGCATCTTCGAGAT
GACGGAAGGCGGCTTGACGGAAGTGAAGAATCCGTCAGAGATGTTCCTTCAGGAACGCCCGCTTGGCGTCTCCGGTTCGA
CGGTCGTCGCCAGCTTGGAAGGGACGCGCCCGGTTCTTGTCGAGATGCAGGCGCTCGTGTCGGCGACGAACTTCCCTTCG
CCGCGGAGAATGTCGACGGGAATCGATCATCAGCGGTTGTCGCTCATCATTGCCGTGCTGGAGAAGCGCATGGGCATGTT
CCTTCAGAACCAGGATGCCTATGTGAATGTGGCCGGCGGGCTGAAGCTCGATGAGCCGGCGGTCGATCTCGCCATTGCCG
TCGCCTTGGCGTCGAGCTTCCGCGATATGCCGACCCAGGCGTTTGACGTCGTCTTCGGCGAAGTAGGGCTGACAGGAGAA
GTGCGCGCGGTGTCCCGTGCGGAGCAGCGCGTGAAGGAAGCCGAGAAGCTGGGCTTCAAGCGGATTATTTTGCCAGAGAA
AAGCCTGCGGGGATGGACCCCTTCTGGACATATTGAGATAGTGGGCGTAAATACGGTAGCGGAAGCATTGACCGCAGCGT
TAGGTTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(62-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-35)

Subunit ChlI of Mg-chelatase (ChlI)

(344-427)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.208

100

0.655
  radA Streptococcus mitis NCTC 12261

58

98.901

0.574
  radA Streptococcus mitis SK321

58

98.901

0.574
  radA Streptococcus pneumoniae Rx1

57.778

98.901

0.571
  radA Streptococcus pneumoniae D39

57.778

98.901

0.571
  radA Streptococcus pneumoniae R6

57.778

98.901

0.571
  radA Streptococcus pneumoniae TIGR4

57.778

98.901

0.571

Multiple sequence alignment