Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MRY16_RS12500 Genome accession   NZ_CP094445
Coordinates   2699118..2699918 (-) Length   266 a.a.
NCBI ID   WP_004908991.1    Uniprot ID   -
Organism   Acinetobacter junii strain A2-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2694118..2704918
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRY16_RS12480 (MRY16_12480) - 2695177..2695875 (-) 699 WP_099949198.1 tetratricopeptide repeat protein -
  MRY16_RS12485 (MRY16_12485) hisS 2695907..2697199 (-) 1293 WP_099949199.1 histidine--tRNA ligase -
  MRY16_RS12490 (MRY16_12490) ispG 2697196..2698311 (-) 1116 WP_004908985.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  MRY16_RS12495 (MRY16_12495) - 2698336..2699127 (-) 792 WP_004950312.1 RodZ domain-containing protein -
  MRY16_RS12500 (MRY16_12500) pilF 2699118..2699918 (-) 801 WP_004908991.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  MRY16_RS12505 (MRY16_12505) rlmN 2699935..2701170 (-) 1236 WP_004950309.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  MRY16_RS12510 (MRY16_12510) ndk 2701292..2701723 (-) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  MRY16_RS12515 (MRY16_12515) iscX 2701831..2702031 (-) 201 WP_004652532.1 Fe-S cluster assembly protein IscX -
  MRY16_RS12520 (MRY16_12520) - 2702114..2702665 (-) 552 WP_243483305.1 phosphatase PAP2 family protein -
  MRY16_RS12525 (MRY16_12525) - 2702693..2704009 (-) 1317 WP_243483308.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30366.44 Da        Isoelectric Point: 9.5600

>NTDB_id=669545 MRY16_RS12500 WP_004908991.1 2699118..2699918(-) (pilF) [Acinetobacter junii strain A2-1]
MNTTRLKTILATTVVFTVLGLTACQSTTLRKDPEKAVQVRTQLAAEYIRTRDLDSAKRSLDQALKIDPRDATANMMMGVL
LQQEGSKPNMEKAEAYFKRAISSEPDNAQARNNYGTYLYQMQRYNEAIEQFTVAGATLGYEQRYQSLENLGRIYLKLGDV
ANAEKSFKQALQANRNSTISMLELSEIFYLQQNNRDASQLYEQYVRNVGQKNQGARALWIGIRIARANADQLGMQVLVNQ
MRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=669545 MRY16_RS12500 WP_004908991.1 2699118..2699918(-) (pilF) [Acinetobacter junii strain A2-1]
TTGAATACAACTCGACTAAAAACGATTTTAGCTACTACTGTTGTGTTTACTGTATTAGGTTTGACTGCTTGCCAATCAAC
AACGTTAAGAAAAGATCCAGAAAAGGCAGTACAAGTCCGAACTCAACTCGCTGCTGAATATATTCGAACACGTGATTTGG
ATTCAGCGAAACGCTCTCTTGATCAAGCTTTAAAAATTGATCCACGTGATGCTACTGCCAATATGATGATGGGTGTTTTG
TTGCAACAAGAAGGCAGCAAACCAAACATGGAGAAAGCAGAAGCTTATTTTAAGCGAGCAATTTCTTCAGAACCCGATAA
TGCTCAAGCTCGTAATAATTACGGTACATATTTGTATCAAATGCAACGTTATAATGAAGCGATTGAGCAATTTACCGTTG
CAGGTGCGACGTTAGGTTACGAACAGCGTTACCAATCGTTGGAAAACTTGGGTCGAATCTATTTAAAACTGGGTGATGTT
GCGAATGCTGAAAAATCATTCAAGCAAGCCTTACAAGCAAATCGAAATTCGACAATTTCAATGTTAGAGTTGTCCGAAAT
TTTCTATTTGCAACAAAATAATAGAGATGCATCGCAACTTTATGAACAATACGTTCGTAATGTCGGGCAGAAAAATCAAG
GTGCTCGTGCACTTTGGATCGGTATACGCATTGCTCGAGCGAATGCGGATCAATTAGGAATGCAAGTATTAGTGAATCAA
ATGCGTGCTTTATTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-69)

Tetratricopeptide repeat (TPR_8)

(145-176)

Tetratricopeptide repeat (TPR_16)

(90-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.026

100

0.793
  pilF Acinetobacter baylyi ADP1

68.045

100

0.68

Multiple sequence alignment