Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   GM3709_RS12440 Genome accession   NZ_AP014821
Coordinates   2913774..2915825 (-) Length   683 a.a.
NCBI ID   WP_066119785.1    Uniprot ID   A0A0D6ASG0
Organism   Geminocystis sp. NIES-3709     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2908774..2920825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GM3709_RS12415 (GM3709_2589) - 2909926..2910675 (-) 750 WP_066119777.1 DUF3887 domain-containing protein -
  GM3709_RS12425 (GM3709_2590) queC 2911193..2911867 (-) 675 WP_255359698.1 7-cyano-7-deazaguanine synthase QueC -
  GM3709_RS12430 (GM3709_2591) - 2911994..2912395 (+) 402 WP_066119780.1 helix-turn-helix transcriptional regulator -
  GM3709_RS12435 (GM3709_2592) cbiB 2912425..2913378 (+) 954 WP_082713068.1 adenosylcobinamide-phosphate synthase CbiB -
  GM3709_RS12440 (GM3709_2593) pilB/pilB1 2913774..2915825 (-) 2052 WP_066119785.1 GspE/PulE family protein Machinery gene
  GM3709_RS21460 - 2916140..2916262 (-) 123 Protein_2503 RNA-directed DNA polymerase -
  GM3709_RS12445 (GM3709_2594) nadC 2916421..2917281 (+) 861 WP_066119786.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GM3709_RS12450 (GM3709_2595) - 2917308..2918201 (-) 894 WP_066119788.1 DUF6515 family protein -
  GM3709_RS12455 (GM3709_2596) - 2918299..2919060 (-) 762 WP_066119790.1 DUF2092 domain-containing protein -
  GM3709_RS12460 (GM3709_2597) - 2919161..2919595 (-) 435 WP_066119792.1 DUF3038 domain-containing protein -
  GM3709_RS12465 (GM3709_2598) - 2919615..2920448 (+) 834 WP_066119795.1 biotin--[acetyl-CoA-carboxylase] ligase -

Sequence

Protein


Download         Length: 683 a.a.        Molecular weight: 76545.51 Da        Isoelectric Point: 5.4231

>NTDB_id=66949 GM3709_RS12440 WP_066119785.1 2913774..2915825(-) (pilB/pilB1) [Geminocystis sp. NIES-3709]
MTQTPISSSTQKQEKLRTALVSPDYFNPFGNKLIQSGFINKEQLKNALVEVRKTKKPLVKVLEQLTQRALSPELVRQYKQ
HHLFELKILYGVDCVDPESEDIASEHMTELIESLVPIQVCRRHKLLPLQRIDGEPPMLVIAMVNPDDLQALDDVKQLLRS
KGLGLQRIVITEEDYDKLLQKYYEEEKERQKIQEAQKQKKELDRLGDVSDIVGDLGDHALDDADQEGGDDLASSSEANQA
PIIELVNKILIKAIQEGASDIHVEPQEENFRVRLRKDGVLRQYFTLPKHIIPAVTARFKIMGELDISERRLPQDGKIRRK
FQGRTVDFRMNTLPSRYGEKVCLRILDNSATQLGLDFLISDQETLTKVRDIASRPFGLILVTGPTGSGKSTSLYSVLAER
NDPGVNISTAEDPIEYALPGITQVQVIREKGMNFASILRAFMRQDPDIILVGETRDAETAKTAIEAALTGHLVLTTLHTN
DAAGAIARLDEMGVEPFMISGALLGVLAQRLMRRVCTQCREPYNPTREELARFGLSASNEESVTFYRAKALNGEALQTAT
NNGTVCIKCGGSGYKGRVGVYEFMVMSERLEKLINQGATTDMIKEAAVEEGMTTILAYSLNLVREGHTTLEEVERVTFTD
SGLESELKAKRKTSLVCSNCNAELEQQWLDCPYCMTPRFSEHH

Nucleotide


Download         Length: 2052 bp        

>NTDB_id=66949 GM3709_RS12440 WP_066119785.1 2913774..2915825(-) (pilB/pilB1) [Geminocystis sp. NIES-3709]
ATGACTCAGACCCCTATTTCTTCTTCTACTCAAAAACAAGAAAAATTAAGAACAGCTTTAGTTAGTCCTGATTATTTTAA
TCCTTTCGGCAATAAATTAATACAATCAGGATTCATTAATAAGGAACAATTAAAAAATGCTCTTGTGGAAGTCAGAAAAA
CGAAAAAACCTCTTGTCAAAGTTTTAGAGCAATTAACTCAAAGGGCATTATCCCCTGAATTAGTCAGACAGTATAAGCAA
CATCATCTTTTTGAACTAAAAATTCTTTATGGTGTTGATTGTGTGGATCCTGAGTCAGAGGACATAGCTTCCGAGCATAT
GACGGAGTTAATCGAGTCTCTTGTACCAATACAAGTATGTCGTCGTCATAAATTATTACCTTTACAACGTATTGACGGTG
AACCTCCCATGTTAGTCATTGCCATGGTTAATCCTGATGATTTACAGGCTTTAGACGATGTGAAACAGTTATTAAGGTCT
AAAGGATTAGGATTACAAAGAATCGTTATTACTGAAGAAGATTATGATAAATTATTACAGAAATATTACGAAGAAGAAAA
AGAACGGCAAAAAATACAGGAAGCTCAGAAACAGAAAAAAGAGTTAGATAGATTAGGCGATGTTAGCGATATTGTCGGTG
ATTTGGGAGATCATGCCCTAGATGATGCTGATCAAGAAGGGGGAGATGATTTAGCCTCATCTAGTGAAGCCAACCAAGCA
CCAATAATTGAGTTAGTCAATAAAATTTTAATTAAAGCGATACAAGAGGGTGCTTCAGATATTCATGTTGAACCTCAAGA
AGAAAATTTCCGGGTGCGGTTGCGTAAAGACGGTGTCTTGCGACAATATTTTACTCTTCCTAAGCATATTATCCCAGCCG
TAACTGCTCGTTTTAAAATTATGGGAGAATTAGATATTTCTGAACGTCGCTTACCCCAAGATGGCAAAATTCGTCGAAAA
TTTCAAGGACGCACAGTTGATTTTCGGATGAACACTTTACCCAGTCGTTATGGAGAAAAAGTTTGTCTGCGGATTCTTGA
CAATTCTGCTACTCAGTTGGGTTTGGATTTTTTGATCAGTGATCAAGAGACATTAACTAAGGTGAGAGATATTGCAAGTC
GTCCTTTTGGTTTGATTTTAGTTACAGGCCCTACTGGATCTGGTAAATCTACCAGTTTATATTCGGTTTTAGCAGAAAGA
AATGATCCGGGGGTTAATATTAGTACAGCAGAAGATCCGATCGAGTATGCTTTACCTGGTATTACTCAGGTTCAGGTAAT
TCGAGAAAAAGGTATGAACTTCGCTTCTATTTTACGAGCATTCATGCGTCAAGATCCTGATATTATTCTTGTTGGAGAAA
CCAGAGATGCTGAAACTGCCAAAACTGCTATCGAAGCCGCTTTAACTGGCCACTTAGTCTTAACCACTTTACATACAAAT
GATGCCGCAGGTGCGATCGCTCGTTTAGATGAAATGGGAGTTGAACCTTTTATGATTTCTGGTGCATTACTAGGAGTCTT
AGCACAACGTCTGATGCGCCGAGTTTGCACCCAATGTAGAGAACCCTATAATCCTACCAGAGAAGAATTAGCCCGTTTTG
GACTATCTGCATCTAATGAAGAATCGGTGACTTTTTATCGTGCTAAAGCATTAAATGGAGAGGCTTTACAGACAGCAACA
AATAATGGAACCGTTTGCATAAAATGTGGCGGTAGTGGTTATAAAGGACGGGTAGGAGTTTATGAGTTTATGGTTATGAG
TGAAAGACTAGAAAAACTAATTAACCAAGGGGCAACCACCGACATGATTAAAGAAGCGGCTGTAGAAGAAGGTATGACGA
CTATTTTGGCTTATAGTCTCAATTTAGTTCGTGAAGGGCATACCACTTTAGAAGAAGTTGAAAGGGTAACATTTACTGAT
TCCGGTTTAGAATCTGAATTAAAAGCTAAACGAAAAACATCTTTGGTTTGTAGTAATTGTAATGCAGAACTAGAGCAACA
GTGGTTAGATTGTCCTTATTGCATGACTCCTCGCTTTAGTGAACATCACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(248-514)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(33-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D6ASG0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

67.781

96.34

0.653

Multiple sequence alignment