Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GM3709_RS08955 Genome accession   NZ_AP014821
Coordinates   2091960..2093072 (-) Length   370 a.a.
NCBI ID   WP_066118459.1    Uniprot ID   A0A0D6AQM7
Organism   Geminocystis sp. NIES-3709     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2086960..2098072
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GM3709_RS08940 (GM3709_1868) - 2088330..2090201 (-) 1872 WP_066118453.1 secretin and TonB N-terminal domain-containing protein -
  GM3709_RS08945 (GM3709_1869) - 2090297..2091121 (-) 825 WP_066118455.1 type II and III secretion system protein -
  GM3709_RS08950 (GM3709_1870) - 2091118..2091957 (-) 840 WP_066118457.1 PilN domain-containing protein -
  GM3709_RS08955 (GM3709_1871) pilM 2091960..2093072 (-) 1113 WP_066118459.1 type IV pilus assembly protein PilM Machinery gene
  GM3709_RS08960 (GM3709_1872) - 2093313..2093675 (+) 363 WP_231937537.1 hypothetical protein -
  GM3709_RS08965 (GM3709_1873) - 2093696..2094868 (-) 1173 WP_066118461.1 glycoside hydrolase family 10 protein -
  GM3709_RS08970 (GM3709_1874) - 2094982..2095677 (+) 696 WP_066118463.1 hypothetical protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41063.17 Da        Isoelectric Point: 4.3096

>NTDB_id=66944 GM3709_RS08955 WP_066118459.1 2091960..2093072(-) (pilM) [Geminocystis sp. NIES-3709]
MLSFFDNLLGGGSNGVGIEINPDKITLAQLAKKGQQYKLLKYHSIDVPDNIFEEGQIVDSPGLAELIDQLLKEAKVKPKQ
IATAVPMKESIIRIIPIPAELNDQELRDQVLNHEASLYLPYPREEVDLDYQKLGYFVDDDGIEKVQVLLVATRREVTDLY
LETFQQAGLKVNVLEINSFALIRTIKEQLKQFSPSEAVVLVDIEFDTTEIAIIVEGVPQFSRTIPIGTYQMQTALAQAMN
LPTSKNTELLQEITIPNNPEQTTTNTTSSQTWINPGMDSLLRVLGELVDELRRSINFYLNQSGDVEVVQLLLAGPGSGLA
QIDEFFTKKLSLPAMQIDPISALSLTFKEDLSVMQRAGLGTVIGLGMRMS

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=66944 GM3709_RS08955 WP_066118459.1 2091960..2093072(-) (pilM) [Geminocystis sp. NIES-3709]
ATGTTAAGTTTTTTTGACAATTTGCTTGGAGGAGGCAGTAATGGAGTAGGAATAGAAATTAATCCTGACAAAATTACCCT
AGCTCAACTAGCAAAAAAAGGACAACAATATAAACTTTTAAAATATCATTCGATCGATGTTCCGGATAATATTTTTGAAG
AGGGACAAATTGTTGATTCTCCCGGATTGGCAGAATTAATCGATCAATTATTGAAGGAAGCGAAAGTTAAACCCAAACAA
ATCGCTACCGCCGTACCAATGAAAGAGTCGATTATTCGTATTATTCCCATTCCTGCTGAATTAAATGATCAGGAATTACG
GGATCAAGTACTAAACCATGAGGCTAGTCTTTATCTACCCTATCCCAGAGAAGAAGTCGATCTCGATTATCAAAAATTGG
GCTATTTTGTAGATGATGATGGCATTGAAAAAGTACAAGTTTTATTAGTGGCAACTCGTCGAGAAGTTACTGATTTATAT
TTGGAGACTTTTCAACAAGCGGGTTTAAAAGTTAATGTGCTAGAAATCAATAGTTTTGCTTTAATTAGAACTATCAAAGA
GCAATTAAAACAATTTTCTCCCAGTGAAGCAGTGGTTTTAGTAGATATTGAATTTGATACTACAGAAATCGCTATTATTG
TGGAAGGTGTGCCTCAATTTTCTCGAACAATTCCCATCGGTACTTATCAAATGCAAACGGCTTTAGCCCAAGCCATGAAT
TTACCCACTTCTAAAAATACAGAATTACTTCAAGAGATTACGATCCCTAATAATCCAGAGCAAACGACAACTAATACCAC
CAGTAGTCAAACATGGATTAATCCGGGGATGGATTCTCTGTTAAGGGTTTTAGGGGAATTAGTAGATGAATTGCGTCGAT
CGATCAATTTCTATCTTAACCAAAGTGGTGATGTAGAAGTAGTACAATTGCTTTTAGCAGGACCTGGTTCCGGATTAGCT
CAAATTGACGAGTTTTTTACTAAAAAATTGAGTCTTCCAGCAATGCAGATTGATCCAATTTCGGCTTTATCTTTAACATT
TAAAGAGGATTTATCTGTCATGCAAAGAGCCGGATTAGGTACAGTAATCGGTTTAGGTATGAGGATGAGTTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-368)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D6AQM7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

66.125

99.73

0.659

Multiple sequence alignment