Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GM3708_RS00325 Genome accession   NZ_AP014815
Coordinates   68177..69289 (+) Length   370 a.a.
NCBI ID   WP_066342875.1    Uniprot ID   A0A0D6AA41
Organism   Geminocystis sp. NIES-3708     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 63177..74289
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GM3708_RS00305 (GM3708_62) - 64513..65451 (-) 939 WP_066342865.1 glycosyltransferase family 2 protein -
  GM3708_RS00310 (GM3708_63) lspA 65500..65964 (-) 465 WP_066342867.1 signal peptidase II -
  GM3708_RS00315 (GM3708_64) - 66163..66786 (-) 624 WP_071827546.1 biotin transporter BioY -
  GM3708_RS00320 (GM3708_65) bioB 66812..67858 (-) 1047 WP_396229674.1 biotin synthase BioB -
  GM3708_RS00325 (GM3708_66) pilM 68177..69289 (+) 1113 WP_066342875.1 type IV pilus assembly protein PilM Machinery gene
  GM3708_RS00330 (GM3708_67) - 69292..70122 (+) 831 WP_066342877.1 PilN domain-containing protein -
  GM3708_RS00335 (GM3708_68) - 70119..70898 (+) 780 WP_066342879.1 type II and III secretion system protein -
  GM3708_RS00340 (GM3708_69) - 71062..73263 (+) 2202 WP_066342881.1 secretin and TonB N-terminal domain-containing protein -
  GM3708_RS19710 (GM3708_70) - 73401..74111 (+) 711 WP_066342887.1 rubrerythrin family protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41118.36 Da        Isoelectric Point: 4.3281

>NTDB_id=66919 GM3708_RS00325 WP_066342875.1 68177..69289(+) (pilM) [Geminocystis sp. NIES-3708]
MLSFLENLIGGKSSGVGIEINPDKITLAQLTKKGQQYKLLKYHSIDVPEDIFEEGQIVDSPGLAELIDQLLKEAKIKPKQ
VATAVPMKESIIRIIPIPAELNDQELRDMVLNHEASLYLPYPREEVDLDFQKLGYFVDDDGIEKVQVLLVATRREVTDLY
LETFQQAGLKVNVLEINSFALMRTIKEQLKQFSPSEAVVLVDIEFDTTEIAIIVEGVPQFSRTIPIGTYQMQTALAQSMN
LPNSKNTELLQEITIPNNPEQTTTNTTSSQTWINPGMDSLLRVLGELVDELRRSINFYLNQSGDVEVVQLLLAGPGSGLT
QIDEFFTKKLSLPAMQVDPVAALSLTLKDELSVMQRAGLGTVIGLGMRMS

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=66919 GM3708_RS00325 WP_066342875.1 68177..69289(+) (pilM) [Geminocystis sp. NIES-3708]
ATGCTAAGTTTTTTAGAAAATTTGATAGGAGGAAAAAGTAGTGGCGTTGGGATTGAAATAAATCCTGATAAAATTACTTT
AGCTCAATTGACGAAAAAAGGGCAACAATATAAACTTTTAAAATATCATTCTATTGATGTTCCTGAAGATATATTCGAGG
AAGGACAAATTGTTGATTCTCCCGGATTAGCGGAATTAATTGATCAATTATTAAAAGAAGCAAAAATTAAACCTAAACAA
GTAGCTACCGCAGTGCCAATGAAAGAATCCATTATTCGGATTATTCCTATTCCTGCGGAGTTAAATGATCAAGAATTACG
AGATATGGTGTTAAATCACGAAGCTAGTCTTTATCTTCCCTATCCTAGAGAAGAAGTAGATCTCGATTTTCAAAAATTAG
GTTATTTTGTAGATGATGACGGTATTGAAAAAGTACAGGTTTTATTAGTCGCCACCCGTAGAGAAGTAACTGATTTATAC
TTAGAAACTTTTCAACAGGCAGGATTAAAAGTTAATGTACTGGAAATTAACAGTTTCGCTTTAATGAGAACAATCAAAGA
ACAATTAAAGCAGTTTTCTCCCAGTGAAGCGGTAGTATTAGTTGATATTGAATTTGATACTACGGAAATTGCTATTATCG
TTGAAGGTGTCCCTCAATTTTCTCGTACTATTCCCATTGGTACTTATCAGATGCAAACGGCTTTAGCCCAATCTATGAAT
TTACCAAATTCTAAGAATACGGAATTATTACAAGAAATTACTATTCCTAACAATCCTGAACAAACTACCACAAACACTAC
CAGTAGTCAGACATGGATTAATCCGGGTATGGATTCACTTTTAAGAGTTTTAGGGGAATTAGTCGATGAATTACGCAGAT
CGATCAATTTCTATTTAAACCAAAGTGGTGATGTTGAAGTAGTACAGTTGCTTTTAGCTGGACCTGGTTCAGGTTTAACA
CAAATTGATGAATTTTTTACCAAAAAATTGAGTCTTCCAGCCATGCAAGTTGATCCTGTAGCCGCTTTATCTCTAACATT
AAAAGATGAATTATCCGTCATGCAACGGGCAGGATTAGGTACAGTAATTGGTTTAGGCATGAGGATGAGTTAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-368)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D6AA41

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

66.667

99.73

0.665

Multiple sequence alignment