Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MRB47_RS02090 Genome accession   NZ_CP094346
Coordinates   436950..438527 (+) Length   525 a.a.
NCBI ID   WP_252101774.1    Uniprot ID   -
Organism   Diaphorobacter sp. LI3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 431950..443527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRB47_RS02065 (MRB47_02065) argS 432237..433940 (+) 1704 WP_041835853.1 arginine--tRNA ligase -
  MRB47_RS02070 (MRB47_02070) - 433953..434579 (+) 627 WP_047349343.1 SPOR domain-containing protein -
  MRB47_RS02075 (MRB47_02075) - 434638..435288 (+) 651 WP_011803808.1 thiol:disulfide interchange protein DsbA/DsbL -
  MRB47_RS02080 (MRB47_02080) lptA 435462..436121 (+) 660 WP_041838776.1 lipopolysaccharide transport periplasmic protein LptA -
  MRB47_RS02085 (MRB47_02085) lptB 436145..436897 (+) 753 WP_088888621.1 LPS export ABC transporter ATP-binding protein -
  MRB47_RS02090 (MRB47_02090) rpoN/rpoN1 436950..438527 (+) 1578 WP_252101774.1 RNA polymerase factor sigma-54 Machinery gene
  MRB47_RS02095 (MRB47_02095) - 438531..438974 (+) 444 WP_011803812.1 acyl-CoA thioesterase -
  MRB47_RS02100 (MRB47_02100) - 438971..440284 (+) 1314 WP_252101775.1 class I SAM-dependent RNA methyltransferase -
  MRB47_RS02105 (MRB47_02105) - 440274..440759 (+) 486 WP_220638394.1 putative toxin-antitoxin system toxin component, PIN family -
  MRB47_RS02110 (MRB47_02110) - 440747..442048 (+) 1302 WP_252101776.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  MRB47_RS02115 (MRB47_02115) - 442530..443360 (+) 831 WP_011803816.1 YecA family protein -

Sequence

Protein


Download         Length: 525 a.a.        Molecular weight: 57419.55 Da        Isoelectric Point: 4.6009

>NTDB_id=669141 MRB47_RS02090 WP_252101774.1 436950..438527(+) (rpoN/rpoN1) [Diaphorobacter sp. LI3]
MKPGLSLRVSQHLALTPQLQQSIRLLQLSTLELAQEVEQMLDENPFLERTLEEAPREDFGLAQADAPVSEQDREAEDAIY
SGAVSAGQESATSQNDAETSTAETTAEPADWEGDGSVDLAPDDSEWGGDAPARNGAGAGSDDEVDATELARSQESLTAFL
HRQALALRLSELDMAALRFLIESLNDDGYLDESLQDLAASLAGTEDPEQLDELVHRFTVALRLLQSLEPAGVGARGLAEC
LTLQLRAMVQDEEGDPDVIQAALAICGQPLDLLAKRDVRRLAQACGTSEERTRAAMALIARLEPRPGRRFADVDRNIIVP
DVIVKKTGRGGQQNFSVQLNPDVMPRLRVHDIYAGALRGGRGEGHQALQARLQEARWFIKNIQQRFDTILRVSRAIVERQ
KNFFVHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTETGGNASSTAVRALIKQFIAAE
NPHKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL

Nucleotide


Download         Length: 1578 bp        

>NTDB_id=669141 MRB47_RS02090 WP_252101774.1 436950..438527(+) (rpoN/rpoN1) [Diaphorobacter sp. LI3]
ATGAAGCCCGGCCTGTCGCTGCGCGTCTCGCAGCACCTGGCGCTCACACCGCAGTTGCAGCAGTCGATCCGCCTGCTGCA
GCTGTCCACGCTGGAGCTGGCCCAGGAGGTCGAGCAGATGCTCGACGAGAACCCCTTCCTGGAGCGCACGCTGGAAGAGG
CCCCGCGCGAGGATTTCGGCCTGGCCCAGGCCGATGCACCGGTGAGCGAGCAAGACCGCGAAGCCGAAGATGCTATTTAT
TCAGGAGCTGTCAGCGCAGGACAGGAAAGCGCTACAAGCCAAAATGATGCAGAAACCTCCACCGCCGAAACGACGGCCGA
ACCCGCGGACTGGGAAGGCGACGGCTCGGTAGACCTGGCGCCCGACGACAGCGAGTGGGGCGGCGACGCCCCGGCGCGCA
ACGGTGCCGGCGCGGGGAGCGACGACGAGGTGGACGCCACCGAGCTGGCCCGCAGCCAGGAGTCGCTCACGGCCTTCCTG
CACCGCCAGGCGCTGGCGCTGCGCCTGTCCGAGCTGGACATGGCCGCGCTGCGCTTCCTCATCGAATCGCTGAACGACGA
CGGCTACCTGGACGAGTCGCTGCAGGACCTGGCCGCCAGCCTGGCGGGCACCGAAGACCCCGAGCAACTCGATGAACTGG
TGCACCGTTTCACCGTGGCGCTGCGCCTGCTGCAAAGCCTGGAGCCCGCGGGCGTGGGCGCGCGCGGACTGGCCGAATGC
CTCACGCTGCAGTTGCGTGCGATGGTCCAGGACGAAGAGGGCGACCCCGACGTGATCCAGGCCGCGCTGGCCATCTGCGG
ACAGCCGCTGGACCTGCTGGCCAAGCGCGACGTGCGCCGCCTGGCGCAGGCCTGCGGCACCAGCGAAGAGCGCACGCGTG
CCGCGATGGCTTTGATTGCGCGGCTGGAGCCACGCCCGGGCAGGCGCTTTGCCGACGTGGACCGCAACATCATCGTGCCC
GACGTGATCGTCAAGAAAACCGGCCGCGGCGGACAGCAGAACTTCAGCGTGCAGCTCAACCCGGACGTGATGCCGCGCCT
GCGCGTGCACGACATCTACGCCGGCGCGTTGCGCGGCGGCCGTGGCGAAGGCCACCAGGCGCTGCAGGCACGGCTGCAGG
AGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGTTTTGACACCATCCTGCGCGTGTCGCGCGCCATCGTGGAGCGGCAG
AAGAACTTCTTCGTGCATGGCGAACTGGCCATGCGCCCGCTGGTGCTGCGCGACATTGCCGATGAACTGGGCCTGCACGA
GTCCACCATCAGCCGCGTGACCACCGCCAAGTACATGGCCACGCCGCAAGGCACCTACGAACTCAAGTATTTCTTCGGCT
CGGGGCTGGGCACCGAAACCGGCGGCAACGCCTCCAGCACGGCCGTGCGCGCGCTCATCAAGCAGTTCATCGCGGCCGAG
AACCCGCACAAGCCGCTGTCGGACAGCCAGATCGCCGAGATGCTCAAGGAGCAGGGCATTGAATGTGCGCGCCGCACCGT
GGCCAAATACCGTGAGGCGCTCAAGATCGCCCCGGCCAACCTCCGCAAGGCACTTTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(367-523)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(147-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.662

100

0.528

Multiple sequence alignment