Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MRB47_RS01030 Genome accession   NZ_CP094346
Coordinates   223845..225227 (+) Length   460 a.a.
NCBI ID   WP_011803649.1    Uniprot ID   A0AAX1WWC5
Organism   Diaphorobacter sp. LI3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 218845..230227
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRB47_RS01005 (MRB47_01005) alr 219488..220585 (+) 1098 WP_252101657.1 alanine racemase -
  MRB47_RS01010 (MRB47_01010) - 220594..221427 (+) 834 WP_252101658.1 hypothetical protein -
  MRB47_RS01015 (MRB47_01015) - 221666..222685 (+) 1020 WP_103736850.1 cytochrome-c peroxidase -
  MRB47_RS01020 (MRB47_01020) - 222790..223404 (-) 615 WP_252101659.1 glutathione S-transferase family protein -
  MRB47_RS01025 (MRB47_01025) - 223553..223735 (-) 183 WP_012655300.1 hypothetical protein -
  MRB47_RS01030 (MRB47_01030) radA 223845..225227 (+) 1383 WP_011803649.1 DNA repair protein RadA Machinery gene
  MRB47_RS01035 (MRB47_01035) - 225297..225737 (+) 441 WP_047350206.1 hypothetical protein -
  MRB47_RS01040 (MRB47_01040) - 225784..226734 (+) 951 WP_252101660.1 branched-chain amino acid transaminase -
  MRB47_RS01045 (MRB47_01045) - 226737..226943 (+) 207 WP_252101661.1 zinc-finger domain-containing protein -
  MRB47_RS01050 (MRB47_01050) - 226959..227774 (+) 816 WP_252101662.1 glycosyltransferase family 2 protein -
  MRB47_RS01055 (MRB47_01055) - 227771..229054 (+) 1284 WP_142085357.1 O-antigen ligase family protein -
  MRB47_RS01060 (MRB47_01060) - 229067..230041 (+) 975 WP_252101663.1 glycosyltransferase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 47828.30 Da        Isoelectric Point: 6.8876

>NTDB_id=669139 MRB47_RS01030 WP_011803649.1 223845..225227(+) (radA) [Diaphorobacter sp. LI3]
MAKEKTIFSCTECGGTSPRWLGKCPSCGAWNTLIESVPDAGPGKNRLSGAGQYAGLAQAQAVMPLAAIEATEVARTASGI
EELDRVLGGGVVEGGVVLIGGDPGIGKSTLLLQAMDALQRAGLPTLYVTGEESGAQVALRSRRLGLDASQVNVLAEIQLE
KILATVEATQPAVCVIDSIQTVYSDQLTSAPGSVAQVRECAAHLTRMAKATGIAVILVGHVTKEGALAGPRVLEHMVDTV
LYFEGDTHSSFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVANPSAIFLSQHSEPVPGSCVLVTLEGTRPMLVEIQALVDG
GGPSPRRLSVGLERDRLAMLLAVLHRHAGVACADQDVFVNAVGGVRISEPAADLAVMLAITSSLRGKALPKGFIAFGEVG
LAGEVRPAPRGQERLKEAAKLGFTVAVVPKANAPKKPIAGLEIHAVERVDEAMTAVRGLD

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=669139 MRB47_RS01030 WP_011803649.1 223845..225227(+) (radA) [Diaphorobacter sp. LI3]
ATGGCCAAAGAAAAAACCATCTTCTCCTGCACCGAATGCGGTGGCACCAGTCCGCGCTGGCTGGGCAAATGCCCTTCCTG
CGGTGCGTGGAACACCCTGATCGAATCCGTGCCCGACGCTGGACCGGGCAAGAACCGCCTGAGCGGGGCCGGCCAGTACG
CGGGCCTGGCCCAGGCGCAGGCGGTGATGCCGCTGGCCGCCATCGAGGCGACCGAGGTGGCGCGCACGGCGAGCGGCATC
GAGGAACTGGACCGCGTGCTGGGCGGTGGCGTGGTCGAGGGCGGAGTGGTGCTCATCGGCGGCGACCCGGGCATCGGCAA
GTCCACGCTGCTCTTGCAGGCGATGGACGCGCTGCAGCGCGCGGGCCTGCCCACGCTGTACGTGACGGGCGAGGAGAGCG
GCGCCCAGGTGGCGTTGCGTTCGCGCCGGCTGGGGCTGGATGCGAGCCAGGTGAACGTGTTGGCCGAGATCCAACTCGAA
AAAATCCTCGCCACCGTGGAGGCCACGCAGCCCGCGGTGTGCGTGATCGACTCCATCCAGACCGTCTATTCCGACCAGCT
CACCAGCGCACCGGGCTCGGTGGCCCAGGTGCGCGAGTGCGCAGCCCACCTCACGCGCATGGCCAAGGCCACGGGCATCG
CCGTCATCTTGGTGGGCCACGTGACCAAGGAAGGTGCGCTGGCCGGCCCGCGCGTGCTGGAGCACATGGTGGACACGGTG
CTGTACTTCGAGGGCGACACGCACAGCAGCTTCCGTCTGGTGCGCGCCATCAAGAACCGCTTTGGCGCCGTGAACGAGAT
CGGCGTGTTCGCCATGACCGAGAAGGGCCTCAAAGGTGTGGCGAACCCTAGTGCCATTTTCTTGAGCCAGCACAGCGAGC
CGGTGCCCGGCAGCTGCGTGCTGGTCACGCTGGAGGGCACGCGGCCGATGCTGGTGGAGATCCAGGCGCTGGTGGACGGC
GGGGGGCCCAGCCCGCGGCGCCTGTCGGTGGGGCTGGAGCGCGACCGCCTGGCCATGCTGCTGGCCGTGCTGCACCGCCA
CGCGGGCGTGGCCTGCGCCGACCAGGACGTATTCGTCAATGCCGTAGGGGGCGTGCGCATCAGCGAACCCGCGGCCGACC
TGGCCGTGATGCTGGCCATCACCAGCAGCCTGCGCGGCAAGGCGTTGCCCAAGGGCTTCATCGCGTTTGGCGAGGTGGGC
CTGGCTGGCGAGGTGCGTCCCGCGCCGCGTGGCCAGGAGCGCCTGAAGGAAGCCGCCAAACTGGGCTTCACGGTGGCCGT
GGTGCCCAAAGCCAATGCGCCCAAGAAGCCGATCGCCGGGCTGGAGATCCACGCAGTGGAGCGCGTGGACGAGGCCATGA
CTGCCGTGCGCGGGCTGGACTGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(68-225)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(363-454)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.904

99.13

0.485
  radA Streptococcus mitis NCTC 12261

47.046

99.348

0.467
  radA Streptococcus pneumoniae D39

48.848

94.348

0.461
  radA Streptococcus pneumoniae TIGR4

48.848

94.348

0.461
  radA Streptococcus mitis SK321

48.848

94.348

0.461
  radA Streptococcus pneumoniae R6

48.848

94.348

0.461
  radA Streptococcus pneumoniae Rx1

48.848

94.348

0.461

Multiple sequence alignment