Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   MRS49_RS17605 Genome accession   NZ_CP094294
Coordinates   3447774..3448673 (+) Length   299 a.a.
NCBI ID   WP_032874887.1    Uniprot ID   -
Organism   Bacillus velezensis strain AP3     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3442774..3453673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRS49_RS17585 - 3443464..3445167 (+) 1704 WP_032874885.1 hypothetical protein -
  MRS49_RS17590 - 3445164..3445445 (+) 282 WP_007409769.1 FlhB-like flagellar biosynthesis protein -
  MRS49_RS17595 sucC 3445620..3446777 (+) 1158 WP_003154283.1 ADP-forming succinate--CoA ligase subunit beta -
  MRS49_RS17600 sucD 3446806..3447708 (+) 903 WP_003154281.1 succinate--CoA ligase subunit alpha -
  MRS49_RS17605 dprA 3447774..3448673 (+) 900 WP_032874887.1 DNA-processing protein DprA Machinery gene
  MRS49_RS17610 topA 3448855..3450930 (+) 2076 WP_029325907.1 type I DNA topoisomerase -
  MRS49_RS17615 trmFO 3450995..3452302 (+) 1308 WP_012117550.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  MRS49_RS17620 xerC 3452372..3453289 (+) 918 WP_007409774.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 299 a.a.        Molecular weight: 32853.99 Da        Isoelectric Point: 8.1570

>NTDB_id=668696 MRS49_RS17605 WP_032874887.1 3447774..3448673(+) (dprA) [Bacillus velezensis strain AP3]
MDQASRCLMVCSINQIISPSLLLKWWKADHSLSFLPDPHPLTVLSEGKTAPEAIFREIERKNSELDEVLSDYRREGITVI
PISSSRYPTWLKAIYDPPAVLYAKGNTLLLEKGRKIGIVGTRKPTEDGIKAVGHLSAELSKKGWVIVSGLASGIDGLSHK
ASIRAKGLTIGVIAGGFHHIYPRENLLLAEYMAEHHLLLSEHPPETKPKKWHFPMRNRIISGLSEGIVVVQGKEKSGSLI
TAYQALDQGREVFAVPGSIFNPYSGGPIKLIQEGAKAVLCAEDIDGELTARCVQYTEPF

Nucleotide


Download         Length: 900 bp        

>NTDB_id=668696 MRS49_RS17605 WP_032874887.1 3447774..3448673(+) (dprA) [Bacillus velezensis strain AP3]
TTGGATCAAGCATCGCGCTGTTTAATGGTCTGCAGTATTAATCAAATCATTTCCCCGTCTCTTCTATTAAAATGGTGGAA
AGCTGATCACTCTCTGTCTTTTTTACCGGATCCGCATCCATTAACTGTTTTATCAGAAGGGAAAACAGCCCCGGAAGCAA
TTTTTCGGGAAATAGAGCGCAAGAATTCGGAACTTGATGAAGTTCTGTCCGATTACCGCCGCGAAGGCATTACTGTCATT
CCGATTTCGTCAAGCCGCTATCCAACATGGCTTAAAGCGATTTATGATCCGCCGGCTGTCTTGTATGCAAAAGGGAACAC
GCTGCTTCTTGAAAAAGGCAGAAAAATCGGGATTGTAGGAACGCGGAAACCGACGGAAGACGGAATAAAAGCGGTTGGGC
ATCTTTCCGCCGAACTCTCAAAAAAAGGCTGGGTCATTGTAAGCGGGCTTGCATCCGGTATAGACGGATTGTCTCATAAG
GCGAGCATCAGGGCAAAAGGGCTTACGATCGGCGTGATAGCCGGCGGATTCCATCACATCTATCCCCGGGAAAATCTCCT
GTTAGCAGAATACATGGCTGAACACCATCTCCTACTCTCAGAACATCCTCCTGAAACAAAGCCGAAAAAATGGCACTTTC
CGATGAGAAACCGCATAATCAGCGGACTAAGTGAAGGAATTGTGGTCGTGCAGGGAAAAGAAAAAAGCGGTTCATTAATC
ACAGCTTACCAGGCTCTCGATCAAGGCAGAGAGGTATTTGCCGTTCCGGGTTCCATATTTAATCCATATTCCGGAGGACC
TATAAAACTCATTCAAGAAGGGGCGAAAGCTGTATTATGCGCAGAGGATATTGACGGAGAGCTGACCGCCCGATGCGTTC
AGTATACGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(73-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

70.569

100

0.706
  dprA Lactococcus lactis subsp. cremoris KW2

42.045

88.294

0.371

Multiple sequence alignment