Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MRS49_RS11305 Genome accession   NZ_CP094294
Coordinates   2179318..2179755 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain AP3     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2174318..2184755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRS49_RS11275 - 2175172..2176374 (+) 1203 WP_032872800.1 GTP-binding protein -
  MRS49_RS11280 - 2176439..2177575 (+) 1137 WP_032872802.1 zinc-dependent alcohol dehydrogenase -
  MRS49_RS11285 - 2177590..2178024 (+) 435 WP_021494203.1 RDD family protein -
  MRS49_RS11290 - 2178097..2178420 (+) 324 WP_032872805.1 YckD family protein -
  MRS49_RS11295 - 2178640..2178858 (+) 219 Protein_2184 family 1 glycosylhydrolase -
  MRS49_RS11300 nin/comJ 2178899..2179297 (-) 399 WP_032872809.1 competence protein ComJ Regulator
  MRS49_RS11305 nucA/comI 2179318..2179755 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MRS49_RS11310 hxlB 2180112..2180669 (-) 558 WP_032872811.1 6-phospho-3-hexuloisomerase -
  MRS49_RS11315 hxlA 2180666..2181301 (-) 636 WP_014416875.1 3-hexulose-6-phosphate synthase -
  MRS49_RS11320 - 2181533..2181895 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=668671 MRS49_RS11305 WP_003156588.1 2179318..2179755(-) (nucA/comI) [Bacillus velezensis strain AP3]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=668671 MRS49_RS11305 WP_003156588.1 2179318..2179755(-) (nucA/comI) [Bacillus velezensis strain AP3]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAAACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATTAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment