Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   MRS49_RS02180 Genome accession   NZ_CP094294
Coordinates   429106..429720 (-) Length   204 a.a.
NCBI ID   WP_032873955.1    Uniprot ID   -
Organism   Bacillus velezensis strain AP3     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 424106..434720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRS49_RS02150 rpsT 424409..424675 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  MRS49_RS02155 holA 424692..425733 (-) 1042 Protein_428 DNA polymerase III subunit delta -
  MRS49_RS02160 - 425773..425924 (-) 152 Protein_429 hypothetical protein -
  MRS49_RS02165 - 425965..426099 (+) 135 WP_003152870.1 YqzM family protein -
  MRS49_RS02170 comEC 426118..428469 (-) 2352 WP_032873958.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  MRS49_RS02175 - 428470..429039 (-) 570 WP_003152868.1 ComE operon protein 2 -
  MRS49_RS02180 comEA 429106..429720 (-) 615 WP_032873955.1 helix-hairpin-helix domain-containing protein Machinery gene
  MRS49_RS02185 comER 429779..430600 (+) 822 WP_012118027.1 late competence protein ComER -
  MRS49_RS02190 - 430669..431406 (-) 738 WP_032873951.1 class I SAM-dependent methyltransferase -
  MRS49_RS02195 rsfS 431403..431759 (-) 357 WP_007408260.1 ribosome silencing factor -
  MRS49_RS02200 yqeK 431763..432338 (-) 576 WP_032873949.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  MRS49_RS02205 - 432328..432897 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  MRS49_RS02210 yhbY 432908..433198 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  MRS49_RS02215 aroE 433192..434034 (-) 843 WP_032873946.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22265.47 Da        Isoelectric Point: 6.4940

>NTDB_id=668639 MRS49_RS02180 WP_032873955.1 429106..429720(-) (comEA) [Bacillus velezensis strain AP3]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDLKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=668639 MRS49_RS02180 WP_032873955.1 429106..429720(-) (comEA) [Bacillus velezensis strain AP3]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATTTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363

Multiple sequence alignment