Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MOW12_RS02860 Genome accession   NZ_CP094254
Coordinates   605453..606250 (+) Length   265 a.a.
NCBI ID   WP_104470591.1    Uniprot ID   -
Organism   Acinetobacter indicus strain BDT2076     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 600453..611250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MOW12_RS02830 (MOW12_02830) - 600769..601188 (+) 420 WP_005182377.1 hypothetical protein -
  MOW12_RS02835 (MOW12_02835) - 601356..602639 (+) 1284 WP_127799070.1 glycosyltransferase family 1 protein -
  MOW12_RS02840 (MOW12_02840) - 602672..603223 (+) 552 WP_104470590.1 phosphatase PAP2 family protein -
  MOW12_RS02845 (MOW12_02845) iscX 603318..603518 (+) 201 WP_127799072.1 Fe-S cluster assembly protein IscX -
  MOW12_RS02850 (MOW12_02850) ndk 603628..604059 (+) 432 WP_005182388.1 nucleoside-diphosphate kinase -
  MOW12_RS02855 (MOW12_02855) rlmN 604182..605417 (+) 1236 WP_005182389.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  MOW12_RS02860 (MOW12_02860) pilF 605453..606250 (+) 798 WP_104470591.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  MOW12_RS02865 (MOW12_02865) - 606241..607029 (+) 789 WP_127799074.1 helix-turn-helix domain-containing protein -
  MOW12_RS02870 (MOW12_02870) ispG 607052..608173 (+) 1122 WP_005182396.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  MOW12_RS02875 (MOW12_02875) hisS 608177..609472 (+) 1296 WP_127799076.1 histidine--tRNA ligase -
  MOW12_RS02880 (MOW12_02880) - 609503..610207 (+) 705 WP_005182401.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 265 a.a.        Molecular weight: 29946.85 Da        Isoelectric Point: 8.3587

>NTDB_id=668382 MOW12_RS02860 WP_104470591.1 605453..606250(+) (pilF) [Acinetobacter indicus strain BDT2076]
MYKFTTISTLCVAAWMSTGCQTTTPTLSPKDPEKAIQVRTQLAAEYLKSGDLDSAKRALDQALETDSRDSMANMMMGVLL
QQEGSKLNLEKADTYFKRAISADPNNAQARNNYGTYLYQVERYNDAIEQLSIAGATLGYDQRFRALENVGRIYLKLGDMA
NAEKSFKQALQANRDSYVSMLELAEIFYFNQQTPAASRMYEQFVRAVGQKNQGARALWIGIRVARANGDQLGQQVLVNQL
RALFPESQEYQRYLQLQHSTEAVWK

Nucleotide


Download         Length: 798 bp        

>NTDB_id=668382 MOW12_RS02860 WP_104470591.1 605453..606250(+) (pilF) [Acinetobacter indicus strain BDT2076]
ATGTATAAATTCACAACCATCAGCACATTGTGTGTGGCTGCATGGATGAGCACCGGCTGTCAAACCACAACTCCTACTTT
AAGTCCGAAAGATCCGGAAAAGGCAATTCAGGTGCGTACCCAGCTGGCGGCCGAGTATTTAAAATCGGGTGATTTAGACT
CGGCCAAACGTGCCCTGGATCAGGCACTGGAAACAGATTCGCGTGATTCTATGGCCAATATGATGATGGGCGTGCTGCTA
CAGCAAGAAGGTAGCAAGCTGAATCTGGAGAAGGCCGATACGTATTTCAAGCGTGCCATCTCTGCCGATCCGAACAATGC
GCAGGCGCGTAACAACTATGGTACGTATTTGTATCAGGTTGAACGCTATAATGATGCCATTGAACAGCTGAGTATTGCCG
GGGCAACACTCGGTTATGATCAACGTTTTCGTGCATTGGAAAACGTGGGGCGGATTTACCTGAAACTGGGCGATATGGCG
AATGCTGAAAAATCATTCAAGCAGGCCCTGCAGGCAAACCGTGACTCCTATGTTTCAATGTTAGAGTTGGCGGAAATTTT
TTATTTCAACCAGCAGACTCCTGCCGCATCCAGAATGTATGAACAGTTTGTGCGTGCTGTGGGACAAAAAAATCAAGGTG
CGCGTGCCTTATGGATTGGGATCCGTGTTGCGCGTGCAAATGGCGATCAGTTAGGACAGCAGGTGCTGGTCAATCAGCTC
CGGGCATTGTTCCCTGAAAGCCAGGAATATCAACGTTATTTGCAATTACAGCACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(144-175)

Tetratricopeptide repeat (TPR_17)

(96-128)

Tetratricopeptide repeat (TPR_16)

(42-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.992

97.736

0.762
  pilF Acinetobacter baylyi ADP1

72.119

100

0.732

Multiple sequence alignment