Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MP579_RS22290 Genome accession   NZ_CP094240
Coordinates   4820029..4821771 (+) Length   580 a.a.
NCBI ID   WP_003062195.1    Uniprot ID   -
Organism   Comamonas sp. 7D-2evo2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4815029..4826771
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MP579_RS22260 (MP579_22260) proB 4815396..4816535 (-) 1140 WP_034357531.1 glutamate 5-kinase -
  MP579_RS22265 (MP579_22265) cgtA 4816646..4817761 (-) 1116 WP_034357529.1 Obg family GTPase CgtA -
  MP579_RS22270 (MP579_22270) rpmA 4817918..4818175 (-) 258 WP_003058534.1 50S ribosomal protein L27 -
  MP579_RS22275 (MP579_22275) rplU 4818192..4818503 (-) 312 WP_003058536.1 50S ribosomal protein L21 -
  MP579_RS22280 (MP579_22280) - 4818846..4819775 (+) 930 WP_003062198.1 polyprenyl synthetase family protein -
  MP579_RS22290 (MP579_22290) pilB 4820029..4821771 (+) 1743 WP_003062195.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MP579_RS22295 (MP579_22295) pilC 4821796..4823013 (+) 1218 WP_012837103.1 type II secretion system F family protein Machinery gene
  MP579_RS22300 (MP579_22300) - 4823013..4823882 (+) 870 WP_034357526.1 A24 family peptidase -
  MP579_RS22305 (MP579_22305) coaE 4823886..4824512 (+) 627 WP_034357524.1 dephospho-CoA kinase -
  MP579_RS22310 (MP579_22310) zapD 4824600..4825355 (+) 756 WP_034357522.1 cell division protein ZapD -
  MP579_RS22315 (MP579_22315) - 4825455..4825664 (+) 210 WP_034357520.1 DNA gyrase inhibitor YacG -
  MP579_RS22320 (MP579_22320) - 4825783..4826253 (-) 471 WP_003062186.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 63116.36 Da        Isoelectric Point: 5.1537

>NTDB_id=668282 MP579_RS22290 WP_003062195.1 4820029..4821771(+) (pilB) [Comamonas sp. 7D-2evo2]
MAAADTLQKNTPAPIAIPGLGRALISAGKVSQQAAENAAKKAATGKTPFITELSQSGEISALEIAETISMMFSTPLLDLN
AIDVQQLPRELLDPKISNAYRVLALSKRGNRLTIATADPTQQEAAEQIKFTTQLLVDWVVVEADKLQKLVEQTGKSVSES
LDTYSSSGDFDFDDVKIEDAPEEKDNAVGAEVEDAPVVKFLHKMLLDAFNMRASDLHFEPYEHNYRVRFRIDGELREIAS
PPIAIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIDALGYEPEE
KERLLKAINRPYGMILVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINMPGVNQVNVNEKAGLTFAAALKSFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCAQCKEPAD
IPHDALIDAGYDEAELDGSWVSYKPVGCPACNNGYKGRVGIYQVMPISEEIQRIILRDGSALEIAAQAKAEGVRSLRGSG
LHKVKLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=668282 MP579_RS22290 WP_003062195.1 4820029..4821771(+) (pilB) [Comamonas sp. 7D-2evo2]
ATGGCTGCTGCCGACACTCTGCAAAAGAACACTCCTGCGCCCATTGCCATCCCAGGTTTGGGCAGGGCCCTGATTTCCGC
AGGAAAGGTTTCGCAGCAAGCCGCCGAGAACGCAGCCAAGAAAGCCGCCACAGGCAAGACCCCGTTCATCACCGAGCTCA
GCCAGTCTGGTGAAATCAGCGCGCTGGAAATTGCCGAAACCATTTCCATGATGTTCAGCACTCCGCTGCTGGACCTCAAC
GCCATCGACGTCCAGCAACTTCCGCGCGAGCTGCTGGACCCGAAAATCAGCAACGCCTATCGCGTGCTGGCACTCAGCAA
GCGCGGCAATCGGCTCACCATCGCCACGGCAGACCCTACTCAGCAGGAAGCTGCCGAACAGATCAAATTCACGACTCAGT
TGCTGGTGGACTGGGTGGTTGTCGAGGCCGACAAGCTGCAAAAGCTCGTCGAGCAGACCGGCAAGAGCGTCAGCGAATCT
CTGGACACCTATTCCAGTTCTGGGGACTTTGACTTTGACGACGTCAAGATCGAGGATGCCCCCGAGGAAAAAGACAATGC
CGTCGGCGCCGAGGTCGAAGATGCGCCGGTCGTCAAGTTTCTGCACAAGATGCTGCTGGACGCCTTCAATATGCGCGCGT
CCGACTTGCACTTCGAGCCCTACGAACACAATTACCGCGTGCGCTTTCGTATCGACGGCGAGCTGCGCGAGATTGCCTCT
CCACCGATTGCGATCAAGGACAAGCTGGCTTCGCGCATCAAGGTGATCTCGCGCCTGGACATCTCGGAGAAACGCGTTCC
GCAAGACGGCCGCATGAAGCTCAAGGTCGGCCCTGATCGCGTCATCGACTTTCGTGTCAGCACCTTGCCCACGCTGTTTG
GCGAAAAGATCGTGATCCGTATTCTGGACCCGAGCTCGGCCAAGATGGGCATCGATGCCCTCGGCTACGAACCCGAGGAA
AAAGAGCGCCTGCTCAAGGCCATCAACCGCCCCTACGGCATGATTCTGGTTACCGGCCCCACGGGCTCGGGCAAGACCGT
GTCGCTCTACACCTGCCTGAACCTGCTCAACCAGCCGGGCGTGAACATTGCCACCGCCGAAGACCCTTCCGAAATCAACA
TGCCCGGCGTCAACCAGGTGAATGTGAACGAGAAGGCCGGCCTGACCTTTGCCGCTGCGCTCAAGTCCTTTCTGCGCCAG
GACCCGGACATCATCATGGTCGGTGAAATCCGTGACCTGGAAACCGCCGACATCTCGATCAAGGCCGCCCAGACCGGGCA
CCTGGTGCTGTCCACGCTGCACACCAATGACGCTCCGACCACGCTGACGCGCATGCGCAATATGGGCATCGCCCCTTTCA
ATATTGCCTCCAGCGTGATCCTGATCACCGCCCAGCGTCTGGCGCGACGCCTGTGCGCGCAGTGCAAGGAACCCGCCGAC
ATACCGCACGACGCCCTGATCGATGCCGGCTACGACGAAGCCGAACTCGACGGCAGCTGGGTCAGCTACAAGCCTGTCGG
CTGCCCTGCCTGCAACAACGGCTACAAGGGACGTGTCGGTATTTATCAGGTGATGCCCATCAGCGAAGAAATTCAGCGCA
TCATCCTGCGTGACGGCAGCGCCCTGGAGATTGCCGCACAGGCCAAGGCCGAGGGAGTACGCTCTCTGCGCGGCTCGGGC
CTGCATAAAGTCAAGCTGGGTCTGACCTCACTCGAAGAAGTGCTGGCGGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.53

98.966

0.54
  pilB Acinetobacter baumannii D1279779

55.203

97.759

0.54
  pilF Neisseria gonorrhoeae MS11

52.473

97.586

0.512
  pilB Legionella pneumophila strain ERS1305867

51.06

97.586

0.498
  pilB Vibrio cholerae strain A1552

46.898

94.483

0.443
  pilB Vibrio campbellii strain DS40M4

44.029

96.724

0.426
  pilB Vibrio parahaemolyticus RIMD 2210633

46.038

91.379

0.421
  pilF Thermus thermophilus HB27

37.755

100

0.383
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.421

98.276

0.378

Multiple sequence alignment