Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MP576_RS02640 Genome accession   NZ_CP094239
Coordinates   569198..570799 (+) Length   533 a.a.
NCBI ID   WP_034355230.1    Uniprot ID   -
Organism   Comamonas sp. 7D-2evo1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 564198..575799
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MP576_RS02620 (MP576_02620) - 565971..566675 (+) 705 WP_034355240.1 SPOR domain-containing protein -
  MP576_RS02625 (MP576_02625) - 566836..567486 (+) 651 WP_034355235.1 thiol:disulfide interchange protein DsbA/DsbL -
  MP576_RS02630 (MP576_02630) lptA 567617..568273 (+) 657 WP_003068194.1 lipopolysaccharide transport periplasmic protein LptA -
  MP576_RS02635 (MP576_02635) lptB 568270..569052 (+) 783 WP_034355232.1 LPS export ABC transporter ATP-binding protein -
  MP576_RS02640 (MP576_02640) rpoN/rpoN1 569198..570799 (+) 1602 WP_034355230.1 RNA polymerase factor sigma-54 Machinery gene
  MP576_RS02645 (MP576_02645) - 570990..571910 (-) 921 WP_080746150.1 aspartyl/asparaginyl beta-hydroxylase domain-containing protein -
  MP576_RS02650 (MP576_02650) - 572044..573276 (-) 1233 WP_034355228.1 DUF711 family protein -
  MP576_RS02655 (MP576_02655) - 573334..573672 (-) 339 WP_003068205.1 tRNA-binding protein -
  MP576_RS02660 (MP576_02660) - 573852..575150 (+) 1299 WP_034355226.1 class I SAM-dependent RNA methyltransferase -
  MP576_RS02665 (MP576_02665) - 575147..575647 (+) 501 WP_034355224.1 putative toxin-antitoxin system toxin component, PIN family -

Sequence

Protein


Download         Length: 533 a.a.        Molecular weight: 59142.43 Da        Isoelectric Point: 4.6632

>NTDB_id=668253 MP576_RS02640 WP_034355230.1 569198..570799(+) (rpoN/rpoN1) [Comamonas sp. 7D-2evo1]
MKPGLSLRVSQHLSLTPQLQQSIQLLQLSTLELAQEVEQMLVDNPFLERAGDEAETAGDAPVDVANSASEYSTSDGIHAE
RDASQAQQATETVAEASNPETPETLNWEAEGLDGQDGEWGGESSSEWDTPSASSSTRNRDGDDETDAIDLAREHQSLTDF
LHRQALSLHLGAADMAALRFLIESLSDDGYLEDSLQELAQSLAGDDLEEREELEQHFVIALKLLQSLEPVGVGARDLAEC
LQLQLRDLIRTAEDEGTTEAHMNRLLLAQAICAQPMDLLARRDLRKLTSLCGAREEQLREAMALIARLEPRPGRRFVDVE
RHIVVPDVIVRPLRSGSGRVDFSVQLNPEVMPRLRVHDIYAGVLRRHKNSDGHAALQQQLQEARWFIKNIQQRFDTILRV
SEAIVERQKNFFVHGELAMRPLVLRDIADTLGLHESTISRVTTAKYMATPQGTFELKYFFGSGLGTETGGSASSTAVRAL
IKQFVDAEDAKKPLSDAKLADMLKEQGIECARRTVAKYREQLKIPTTTLRKAL

Nucleotide


Download         Length: 1602 bp        

>NTDB_id=668253 MP576_RS02640 WP_034355230.1 569198..570799(+) (rpoN/rpoN1) [Comamonas sp. 7D-2evo1]
ATGAAGCCAGGTCTCTCGCTACGCGTTTCGCAGCATTTATCGCTTACGCCGCAGCTGCAGCAATCCATTCAGCTGCTGCA
GCTTTCCACCCTGGAGCTGGCGCAGGAAGTCGAGCAGATGCTGGTCGACAACCCTTTTCTGGAACGTGCGGGCGATGAGG
CCGAAACTGCGGGAGATGCTCCTGTAGATGTAGCTAATAGCGCCAGTGAATACAGTACTTCAGATGGTATTCATGCTGAA
AGAGATGCATCGCAAGCGCAACAAGCTACTGAAACGGTAGCGGAGGCATCAAACCCCGAAACCCCTGAAACGCTGAACTG
GGAGGCCGAGGGCCTGGACGGCCAGGACGGGGAATGGGGTGGCGAATCCAGCAGCGAGTGGGATACGCCCTCGGCCAGCA
GCTCCACGCGCAACCGCGATGGCGATGACGAAACCGATGCCATCGATCTGGCGCGCGAGCACCAGAGCCTGACCGACTTT
CTCCATCGTCAGGCGCTGAGCCTGCATCTGGGCGCGGCGGACATGGCGGCGCTGCGCTTTCTCATCGAGTCGCTCAGCGA
CGACGGCTATCTCGAGGATTCCCTGCAGGAGCTGGCGCAGAGCCTGGCCGGCGACGACCTGGAAGAGCGCGAGGAACTGG
AGCAGCACTTTGTCATTGCTCTGAAGCTGCTTCAAAGCCTGGAGCCCGTGGGCGTGGGAGCGCGTGATCTGGCCGAGTGC
CTGCAGCTGCAGCTGCGCGACCTGATCCGCACCGCCGAGGACGAGGGCACGACCGAGGCGCATATGAACCGGCTGCTGCT
GGCGCAGGCCATCTGCGCGCAGCCCATGGACCTGCTGGCGCGGCGCGATCTGCGCAAGCTCACCAGCCTGTGCGGCGCGC
GAGAGGAACAGCTGCGCGAAGCCATGGCCCTGATCGCCAGGCTGGAGCCGCGTCCGGGTCGGCGCTTTGTGGACGTGGAG
CGCCATATCGTGGTGCCTGATGTCATCGTGCGCCCGCTGCGGTCCGGCTCGGGCCGGGTGGACTTCTCCGTGCAGCTCAA
CCCCGAGGTCATGCCGCGTCTGCGCGTGCACGACATCTATGCCGGCGTGCTGCGCCGCCACAAGAACAGCGACGGCCATG
CGGCGCTGCAGCAGCAGTTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGTTTCGACACCATCTTGCGGGTC
TCCGAAGCCATTGTGGAGCGGCAGAAGAACTTCTTTGTGCATGGCGAACTGGCCATGCGCCCGCTGGTGCTGCGCGATAT
CGCCGACACCCTGGGTCTGCACGAGTCCACGATCAGCCGCGTGACCACGGCCAAGTACATGGCCACGCCCCAGGGCACCT
TCGAGCTCAAGTATTTCTTCGGCTCGGGCCTGGGCACGGAAACCGGCGGCAGTGCTTCCAGCACGGCCGTGCGTGCGCTG
ATCAAGCAGTTTGTCGATGCCGAAGATGCCAAGAAGCCGCTGTCGGACGCCAAGCTGGCCGATATGCTCAAAGAGCAGGG
CATCGAGTGCGCGCGCCGCACCGTCGCCAAATACCGCGAGCAGCTCAAGATACCGACGACCACGCTGCGCAAGGCGCTGT
AG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(150-360)

Sigma-54, DNA binding domain (Sigma54_DBD)

(375-531)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.028

100

0.512

Multiple sequence alignment