Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MP576_RS00590 Genome accession   NZ_CP094239
Coordinates   132498..133883 (-) Length   461 a.a.
NCBI ID   WP_034351311.1    Uniprot ID   -
Organism   Comamonas sp. 7D-2evo1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 127498..138883
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MP576_RS00560 (MP576_00560) - 127528..128292 (+) 765 WP_034351307.1 glycosyltransferase family 25 protein -
  MP576_RS00565 (MP576_00565) - 128282..129415 (-) 1134 WP_052057547.1 glycosyltransferase -
  MP576_RS00570 (MP576_00570) - 129478..130443 (+) 966 WP_187771846.1 glycosyltransferase family 32 protein -
  MP576_RS00575 (MP576_00575) - 130489..130698 (-) 210 WP_003059801.1 zinc-finger domain-containing protein -
  MP576_RS00580 (MP576_00580) - 130842..131780 (-) 939 WP_034351308.1 branched-chain amino acid transaminase -
  MP576_RS00585 (MP576_00585) - 131862..132317 (-) 456 WP_034351309.1 hypothetical protein -
  MP576_RS00590 (MP576_00590) radA 132498..133883 (-) 1386 WP_034351311.1 DNA repair protein RadA Machinery gene
  MP576_RS00600 (MP576_00600) - 134307..135614 (-) 1308 WP_034351570.1 Glu/Leu/Phe/Val dehydrogenase -
  MP576_RS00605 (MP576_00605) - 135824..136747 (+) 924 WP_034351313.1 fumarylacetoacetate hydrolase family protein -
  MP576_RS00610 (MP576_00610) - 136902..138011 (-) 1110 WP_187771841.1 PPK2 family polyphosphate kinase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 48103.42 Da        Isoelectric Point: 7.6670

>NTDB_id=668250 MP576_RS00590 WP_034351311.1 132498..133883(-) (radA) [Comamonas sp. 7D-2evo1]
MAKEKTTFTCNACGGTSPRWLGKCPGCGAWNTLVETVADSASGGKNRLSQPQGYAGLANAQPVTPLSAIEAQDVARTPSG
IEELDRVLGGGVVEGGVVLIGGDPGIGKSTLLLQAMDALHRIGLPTLYVTGEESGAQVALRSRRLGLDNSQVNVLAEIQL
EKILATVEATQPAVVVIDSIQTVYSDQLSSAPGSVAQVRECAAHLTRAAKTTGITIILVGHVTKDGALAGPRVLEHMVDT
VLYFEGDTHSNFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVTNPSAIFLSQHSEPVPGSCVLVTLEGTRPMLVEIQALVD
QGGPAARRLSVGLDRDRLAMLLAVLNRHAGVACADQDVFVNAVGGVRINEPAADLAVMLAITSSLRGKSLPKGFIAFGEV
GLAGEVRPAPRGQERLKEAAKLGFTIAVVPKANTPKKPIAGLTIHAVERVDEAINIVRGIG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=668250 MP576_RS00590 WP_034351311.1 132498..133883(-) (radA) [Comamonas sp. 7D-2evo1]
ATGGCCAAAGAGAAAACCACTTTCACCTGCAACGCCTGTGGCGGCACCAGCCCGCGCTGGCTGGGCAAATGCCCTGGCTG
CGGCGCCTGGAACACCCTGGTCGAGACCGTGGCCGACTCCGCCTCGGGCGGCAAGAACCGCCTGAGCCAGCCCCAAGGCT
ATGCAGGTCTGGCCAATGCCCAGCCCGTCACTCCGCTGTCGGCCATCGAGGCCCAGGATGTGGCGCGCACCCCCAGCGGT
ATCGAGGAGCTGGACCGCGTGCTGGGCGGCGGCGTGGTCGAAGGCGGCGTGGTGCTGATTGGCGGAGATCCCGGAATAGG
TAAGTCCACACTTCTTTTGCAAGCCATGGATGCATTGCATCGTATCGGTCTGCCCACGCTCTATGTGACGGGCGAGGAAA
GCGGCGCCCAGGTGGCGCTGCGCTCGCGCCGGCTGGGGCTGGACAACAGCCAGGTCAATGTGCTGGCCGAGATCCAGCTC
GAGAAAATCCTGGCCACCGTGGAGGCCACCCAGCCCGCCGTGGTGGTCATCGACTCGATTCAGACCGTGTATTCGGACCA
GCTTTCCTCGGCACCGGGCTCCGTGGCCCAGGTGCGCGAGTGCGCGGCCCATCTGACGCGCGCGGCCAAGACCACGGGCA
TCACCATCATTTTGGTGGGCCATGTGACCAAGGACGGCGCGCTGGCCGGCCCGCGCGTGCTCGAGCACATGGTGGACACG
GTGCTCTATTTCGAAGGCGACACGCACAGCAACTTCCGCCTGGTGCGCGCCATCAAGAACCGCTTTGGCGCCGTCAACGA
GATCGGCGTCTTCGCGATGACAGAAAAAGGCCTCAAGGGCGTGACCAACCCCAGCGCCATCTTCCTGAGCCAGCACAGCG
AGCCCGTGCCCGGCAGCTGCGTGCTGGTGACACTGGAGGGCACGCGCCCCATGCTGGTGGAAATCCAGGCCCTGGTGGAC
CAGGGCGGCCCGGCCGCGCGCCGCCTCTCCGTCGGCCTGGACCGCGACCGGCTGGCCATGCTGCTGGCCGTGCTCAACCG
CCATGCGGGCGTGGCCTGCGCCGACCAGGATGTCTTCGTCAATGCAGTGGGCGGCGTGCGCATCAATGAGCCTGCAGCCG
ACCTGGCGGTGATGTTAGCAATCACTTCCAGCCTGCGTGGCAAGTCACTGCCCAAGGGCTTTATCGCGTTTGGCGAGGTC
GGTCTGGCCGGCGAGGTGCGCCCCGCCCCTCGCGGTCAGGAGCGACTCAAGGAGGCGGCAAAGTTGGGATTCACTATCGC
CGTAGTTCCCAAGGCCAATACCCCCAAGAAGCCCATCGCCGGCCTGACCATCCATGCCGTGGAGCGCGTTGATGAAGCCA
TCAACATCGTGCGCGGCATTGGCTGA

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(364-455)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(68-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.02

99.566

0.488
  radA Streptococcus mitis NCTC 12261

47.817

99.349

0.475
  radA Streptococcus mitis SK321

47.817

99.349

0.475
  radA Streptococcus pneumoniae TIGR4

48.438

97.18

0.471
  radA Streptococcus pneumoniae D39

48.438

97.18

0.471
  radA Streptococcus pneumoniae Rx1

48.438

97.18

0.471
  radA Streptococcus pneumoniae R6

48.438

97.18

0.471

Multiple sequence alignment