Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MPZ60_RS23700 Genome accession   NZ_CP094238
Coordinates   5145646..5147247 (-) Length   533 a.a.
NCBI ID   WP_034355230.1    Uniprot ID   -
Organism   Comamonas sp. 7D-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5140646..5152247
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MPZ60_RS23675 (MPZ60_23675) - 5140798..5141298 (-) 501 WP_034355224.1 putative toxin-antitoxin system toxin component, PIN family -
  MPZ60_RS23680 (MPZ60_23680) - 5141295..5142593 (-) 1299 WP_034355226.1 class I SAM-dependent RNA methyltransferase -
  MPZ60_RS23685 (MPZ60_23685) - 5142773..5143111 (+) 339 WP_003068205.1 tRNA-binding protein -
  MPZ60_RS23690 (MPZ60_23690) - 5143169..5144401 (+) 1233 WP_034355228.1 DUF711 family protein -
  MPZ60_RS23695 (MPZ60_23695) - 5144535..5145455 (+) 921 WP_080746150.1 aspartyl/asparaginyl beta-hydroxylase domain-containing protein -
  MPZ60_RS23700 (MPZ60_23700) rpoN/rpoN1 5145646..5147247 (-) 1602 WP_034355230.1 RNA polymerase factor sigma-54 Machinery gene
  MPZ60_RS23705 (MPZ60_23705) lptB 5147393..5148175 (-) 783 WP_034355232.1 LPS export ABC transporter ATP-binding protein -
  MPZ60_RS23710 (MPZ60_23710) lptA 5148172..5148828 (-) 657 WP_003068194.1 lipopolysaccharide transport periplasmic protein LptA -
  MPZ60_RS23715 (MPZ60_23715) - 5148959..5149609 (-) 651 WP_034355235.1 thiol:disulfide interchange protein DsbA/DsbL -
  MPZ60_RS23720 (MPZ60_23720) - 5149770..5150474 (-) 705 WP_034355240.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 533 a.a.        Molecular weight: 59142.43 Da        Isoelectric Point: 4.6632

>NTDB_id=668247 MPZ60_RS23700 WP_034355230.1 5145646..5147247(-) (rpoN/rpoN1) [Comamonas sp. 7D-2]
MKPGLSLRVSQHLSLTPQLQQSIQLLQLSTLELAQEVEQMLVDNPFLERAGDEAETAGDAPVDVANSASEYSTSDGIHAE
RDASQAQQATETVAEASNPETPETLNWEAEGLDGQDGEWGGESSSEWDTPSASSSTRNRDGDDETDAIDLAREHQSLTDF
LHRQALSLHLGAADMAALRFLIESLSDDGYLEDSLQELAQSLAGDDLEEREELEQHFVIALKLLQSLEPVGVGARDLAEC
LQLQLRDLIRTAEDEGTTEAHMNRLLLAQAICAQPMDLLARRDLRKLTSLCGAREEQLREAMALIARLEPRPGRRFVDVE
RHIVVPDVIVRPLRSGSGRVDFSVQLNPEVMPRLRVHDIYAGVLRRHKNSDGHAALQQQLQEARWFIKNIQQRFDTILRV
SEAIVERQKNFFVHGELAMRPLVLRDIADTLGLHESTISRVTTAKYMATPQGTFELKYFFGSGLGTETGGSASSTAVRAL
IKQFVDAEDAKKPLSDAKLADMLKEQGIECARRTVAKYREQLKIPTTTLRKAL

Nucleotide


Download         Length: 1602 bp        

>NTDB_id=668247 MPZ60_RS23700 WP_034355230.1 5145646..5147247(-) (rpoN/rpoN1) [Comamonas sp. 7D-2]
ATGAAGCCAGGTCTCTCGCTACGCGTTTCGCAGCATTTATCGCTTACGCCGCAGCTGCAGCAATCCATTCAGCTGCTGCA
GCTTTCCACCCTGGAGCTGGCGCAGGAAGTCGAGCAGATGCTGGTCGACAACCCTTTTCTGGAACGTGCGGGCGATGAGG
CCGAAACTGCGGGAGATGCTCCTGTAGATGTAGCTAATAGCGCCAGTGAATACAGTACTTCAGATGGTATTCATGCTGAA
AGAGATGCATCGCAAGCGCAACAAGCTACTGAAACGGTAGCGGAGGCATCAAACCCCGAAACCCCTGAAACGCTGAACTG
GGAGGCCGAGGGCCTGGACGGCCAGGACGGGGAATGGGGTGGCGAATCCAGCAGCGAGTGGGATACGCCCTCGGCCAGCA
GCTCCACGCGCAACCGCGATGGCGATGACGAAACCGATGCCATCGATCTGGCGCGCGAGCACCAGAGCCTGACCGACTTT
CTCCATCGTCAGGCGCTGAGCCTGCATCTGGGCGCGGCGGACATGGCGGCGCTGCGCTTTCTCATCGAGTCGCTCAGCGA
CGACGGCTATCTCGAGGATTCCCTGCAGGAGCTGGCGCAGAGCCTGGCCGGCGACGACCTGGAAGAGCGCGAGGAACTGG
AGCAGCACTTTGTCATTGCTCTGAAGCTGCTTCAAAGCCTGGAGCCCGTGGGCGTGGGAGCGCGTGATCTGGCCGAGTGC
CTGCAGCTGCAGCTGCGCGACCTGATCCGCACCGCCGAGGACGAGGGCACGACCGAGGCGCATATGAACCGGCTGCTGCT
GGCGCAGGCCATCTGCGCGCAGCCCATGGACCTGCTGGCGCGGCGCGATCTGCGCAAGCTCACCAGCCTGTGCGGCGCGC
GAGAGGAACAGCTGCGCGAAGCCATGGCCCTGATCGCCAGGCTGGAGCCGCGTCCGGGTCGGCGCTTTGTGGACGTGGAG
CGCCATATCGTGGTGCCTGATGTCATCGTGCGCCCGCTGCGGTCCGGCTCGGGCCGGGTGGACTTCTCCGTGCAGCTCAA
CCCCGAGGTCATGCCGCGTCTGCGCGTGCACGACATCTATGCCGGCGTGCTGCGCCGCCACAAGAACAGCGACGGCCATG
CGGCGCTGCAGCAGCAGTTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGTTTCGACACCATCTTGCGGGTC
TCCGAAGCCATTGTGGAGCGGCAGAAGAACTTCTTTGTGCATGGCGAACTGGCCATGCGCCCGCTGGTGCTGCGCGATAT
CGCCGACACCCTGGGTCTGCACGAGTCCACGATCAGCCGCGTGACCACGGCCAAGTACATGGCCACGCCCCAGGGCACCT
TCGAGCTCAAGTATTTCTTCGGCTCGGGCCTGGGCACGGAAACCGGCGGCAGTGCTTCCAGCACGGCCGTGCGTGCGCTG
ATCAAGCAGTTTGTCGATGCCGAAGATGCCAAGAAGCCGCTGTCGGACGCCAAGCTGGCCGATATGCTCAAAGAGCAGGG
CATCGAGTGCGCGCGCCGCACCGTCGCCAAATACCGCGAGCAGCTCAAGATACCGACGACCACGCTGCGCAAGGCGCTGT
AG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(150-360)

Sigma-54, DNA binding domain (Sigma54_DBD)

(375-531)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.028

100

0.512

Multiple sequence alignment