Detailed information    

insolico Bioinformatically predicted

Overview

Name   comB4   Type   Machinery gene
Locus tag   MPF99_RS04000 Genome accession   NZ_CP094130
Coordinates   821705..824068 (+) Length   787 a.a.
NCBI ID   WP_245058096.1    Uniprot ID   -
Organism   Helicobacter pylori strain Hpfe053     
Function   transformation-associated type IV transport system (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 816705..829068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MPF99_RS03970 (MPF99_03970) groES 816736..817092 (-) 357 WP_245058088.1 co-chaperone GroES -
  MPF99_RS03975 (MPF99_03975) dnaG 817367..819046 (+) 1680 WP_245058090.1 DNA primase -
  MPF99_RS03980 (MPF99_03980) - 819043..820095 (+) 1053 WP_245058092.1 MnmA/TRMU family protein -
  MPF99_RS03985 (MPF99_03985) - 820186..821013 (+) 828 WP_245058094.1 DUF5718 family protein -
  MPF99_RS03990 (MPF99_03990) comB2 821158..821439 (+) 282 WP_001272686.1 TrbC/VirB2 family protein Machinery gene
  MPF99_RS03995 (MPF99_03995) comB3 821439..821702 (+) 264 WP_000584955.1 hypothetical protein Machinery gene
  MPF99_RS04000 (MPF99_04000) comB4 821705..824068 (+) 2364 WP_245058096.1 VirB4 family type IV secretion/conjugal transfer ATPase Machinery gene
  MPF99_RS04010 (MPF99_04010) - 824369..825709 (+) 1341 WP_021306482.1 hypothetical protein -
  MPF99_RS04015 (MPF99_04015) cheV1 825810..826775 (+) 966 WP_024948933.1 chemotaxis protein CheV1 -
  MPF99_RS04020 (MPF99_04020) nspC 826772..827989 (+) 1218 WP_245058098.1 carboxynorspermidine decarboxylase -
  MPF99_RS04025 (MPF99_04025) lpxE 827986..828558 (-) 573 WP_245058100.1 lipid A 1-phosphatase LpxE -

Sequence

Protein


Download         Length: 787 a.a.        Molecular weight: 90314.04 Da        Isoelectric Point: 9.1045

>NTDB_id=667287 MPF99_RS04000 WP_245058096.1 821705..824068(+) (comB4) [Helicobacter pylori strain Hpfe053]
MLEKLLSAIKQKVSNYFLGVLPKSYSMSEENNILGLYDEHFLLTKNENLVGILRLEGVSYTHLSTEQLQDLFTERQMALD
SLEKVVARLVVKRRKIDHQQNIQTNSKYLQAILNQFENKEVYENQYFLVLESTHSLQGVLEHKKKSLMHANRENFKDILS
YKAHFLQETLKSLEIQLKNYAPKLLSSKEVLNFYAEYINGFDLPLKPLVGGYLSDSYIASSITFEKDYFIQESFNQKTYN
RLIGIKAYESERITSIAIGALLYQETPLDIIFSIEPMSVNKMLSFLKERAKFSMSNLVKNELLEYQELVKTKRLSMQKFA
LNILIKAPSLEDLDTQTSLVLGLLFKENLVGVIETFGLKGGYFSFFPERIHLNHRLRFLTSKALACLMVFERQNLGFKAN
SWGNSPLSVFKNLDYSPFLFNFHNQEVSHNNAKEIARVNGHTLIIGATGSGKSTLISFLMMSALKYQNMRLLAFDRMQGL
YSFTKFFKGHYHDGKSFSINPFCLEPNLQNLEFLQSFFLSMFDLSPSRDKEALEDMNAILSAIKSLYETLYPKAFSLLDF
KETLKRTSSNQLGLSLEPYLNNPLFNALNDAFNSRAFLNVINLDAITQNPKDLGLLAYYLFYKILEESRKNDSGFLVFLD
EFKSYVENDLLNAKINALITQARKANGVVVLALQDIYQLSGVKNAHSFLSNMGTLILYPQKNARELKHHFNVPLSETEIS
FLENTPLYARQVLVKNLGNGSSNMIDVSLEGLGRYLKIFNSDSSHVNKVKALQKDYPTEWREKLLKS

Nucleotide


Download         Length: 2364 bp        

>NTDB_id=667287 MPF99_RS04000 WP_245058096.1 821705..824068(+) (comB4) [Helicobacter pylori strain Hpfe053]
ATGTTAGAAAAGCTTTTGAGCGCTATCAAACAAAAAGTTTCAAACTATTTTTTAGGGGTTTTGCCTAAAAGCTATTCTAT
GAGCGAAGAAAACAACATTTTAGGCTTGTATGATGAGCATTTCTTGCTCACTAAAAACGAAAACTTGGTGGGCATTCTCC
GTTTAGAGGGGGTGAGTTACACCCATTTAAGCACGGAGCAATTGCAAGATCTCTTCACCGAGCGCCAAATGGCGTTGGAT
TCTTTAGAAAAAGTCGTGGCGCGCCTTGTGGTTAAAAGGCGTAAAATTGATCACCAACAAAATATCCAAACCAATTCCAA
ATACTTGCAAGCGATTTTGAATCAATTTGAAAATAAAGAAGTGTATGAAAATCAGTATTTTTTAGTTTTAGAAAGCACGC
ACTCTTTACAAGGCGTTTTAGAGCATAAGAAAAAATCTCTTATGCATGCCAATAGGGAAAACTTTAAGGACATTCTCTCT
TATAAGGCGCATTTTTTGCAAGAAACTTTAAAAAGCTTAGAAATCCAGCTCAAAAACTACGCCCCCAAACTCTTAAGCTC
CAAAGAGGTTTTGAATTTTTATGCGGAATACATTAACGGGTTTGATCTCCCTTTAAAGCCCCTAGTAGGGGGGTATTTGA
GCGATAGTTATATCGCTAGTTCTATCACTTTTGAAAAAGATTATTTCATTCAAGAAAGCTTTAATCAAAAAACCTATAAC
CGCTTGATTGGCATTAAAGCTTATGAGAGCGAGCGCATCACTTCTATAGCGATCGGAGCGCTTTTATACCAAGAAACGCC
TTTAGACATTATCTTTTCTATAGAGCCTATGAGCGTCAATAAAATGCTGAGTTTTTTAAAAGAGAGGGCCAAGTTTAGCA
TGTCCAATCTCGTTAAAAACGAGCTACTAGAATACCAAGAATTGGTCAAAACCAAACGCCTATCCATGCAAAAATTCGCC
CTAAACATTCTTATCAAAGCCCCTAGTTTAGAGGATTTAGACACTCAAACAAGCTTAGTTTTAGGGCTTTTATTTAAAGA
AAACTTAGTGGGCGTTATAGAAACTTTTGGCTTGAAAGGGGGGTATTTTTCCTTTTTCCCTGAACGCATCCATTTAAACC
ACCGCTTGCGTTTTTTAACTTCTAAAGCCCTAGCGTGTTTAATGGTGTTTGAAAGGCAAAATTTAGGTTTTAAGGCTAAT
TCATGGGGGAATAGCCCTTTGAGCGTGTTTAAAAATTTGGATTATTCCCCTTTTTTATTCAATTTCCACAACCAAGAAGT
GAGCCACAACAACGCTAAAGAAATCGCCAGAGTGAATGGGCATACCTTAATTATAGGGGCTACCGGGAGCGGTAAAAGCA
CGCTGATTAGTTTTTTAATGATGAGCGCTTTGAAATACCAAAACATGCGCCTTTTAGCATTTGATAGGATGCAAGGGTTG
TATTCTTTCACAAAATTTTTTAAAGGGCATTACCATGACGGCAAATCTTTTAGCATCAACCCCTTTTGTTTAGAGCCTAA
TTTGCAGAATTTAGAATTTTTGCAATCCTTCTTTTTGAGCATGTTTGATCTTTCCCCTTCAAGGGATAAAGAAGCTTTGG
AAGACATGAATGCGATTTTGAGCGCGATTAAGAGCCTTTATGAGACTTTATACCCTAAAGCTTTTAGTTTGCTAGATTTT
AAAGAAACGCTTAAAAGAACCTCATCTAACCAATTGGGTTTGAGTTTAGAGCCGTATTTGAATAACCCCCTTTTTAATGC
TTTGAATGACGCGTTCAACTCCAGAGCTTTTTTAAATGTGATAAACCTAGATGCCATCACCCAAAACCCTAAAGATTTAG
GGCTTTTAGCCTATTATTTGTTTTATAAAATCTTAGAAGAATCCAGGAAAAACGACAGCGGTTTTTTGGTTTTTTTAGAC
GAGTTTAAATCCTATGTGGAAAACGATTTGTTGAACGCTAAAATTAACGCTTTAATCACGCAAGCCAGAAAGGCTAATGG
CGTGGTGGTGTTGGCTTTGCAAGACATTTACCAATTAAGCGGGGTTAAAAACGCCCATAGTTTTTTAAGCAACATGGGGA
CTCTTATTTTGTATCCGCAAAAAAACGCTAGGGAGTTGAAACACCACTTCAATGTGCCTTTGAGCGAAACTGAAATTTCT
TTTTTAGAAAACACCCCTTTGTATGCCAGGCAGGTTTTAGTCAAAAATCTGGGTAACGGGAGTTCTAACATGATTGATGT
GAGTTTGGAGGGTTTGGGGCGTTATTTGAAAATCTTTAATTCAGACTCTAGCCATGTGAATAAAGTGAAAGCGTTACAAA
AAGACTACCCTACAGAGTGGCGTGAGAAACTTTTGAAGAGCTAG

Domains

Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(176-375)

FtsK/SpoIIIE family (FtsK_SpoIIIE)

(426-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comB4 Helicobacter pylori 26695

97.586

100

0.976

Multiple sequence alignment